Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04870 and MMP_RS04875 are separated by 22 nucleotides MMP_RS04875 and MMP_RS04880 are separated by 64 nucleotides MMP_RS04880 and MMP_RS04885 are separated by 57 nucleotides MMP_RS04885 and MMP_RS04890 are separated by 13 nucleotides
MMP_RS04870: MMP_RS04870 - TIGR02253 family HAD-type hydrolase, at 926,379 to 927,056
_RS04870
MMP_RS04875: MMP_RS04875 - energy-converting hydrogenase B subunit P, at 927,079 to 927,366
_RS04875
MMP_RS04880: MMP_RS04880 - phosphoadenosine phosphosulfate reductase family protein, at 927,431 to 928,942
_RS04880
MMP_RS04885: MMP_RS04885 - sulfite exporter TauE/SafE family protein, at 929,000 to 929,812
_RS04885
MMP_RS04890: MMP_RS04890 - adenylyltransferase/cytidyltransferase family protein, at 929,826 to 930,278
_RS04890
Position (kb) 927
928
929 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 926.431 kb on - strand at 926.432 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.517 kb on + strand, within MMP_RS04870 at 926.568 kb on + strand, within MMP_RS04870 at 926.589 kb on + strand, within MMP_RS04870 at 926.620 kb on - strand, within MMP_RS04870 at 926.621 kb on + strand, within MMP_RS04870 at 926.635 kb on + strand, within MMP_RS04870 at 926.636 kb on - strand, within MMP_RS04870 at 926.637 kb on + strand, within MMP_RS04870 at 926.808 kb on + strand, within MMP_RS04870 at 926.912 kb on - strand, within MMP_RS04870 at 927.073 kb on - strand at 927.073 kb on - strand at 927.073 kb on - strand at 927.074 kb on + strand at 927.076 kb on + strand at 927.083 kb on + strand at 927.118 kb on + strand, within MMP_RS04875 at 927.158 kb on + strand, within MMP_RS04875 at 927.294 kb on + strand, within MMP_RS04875 at 927.313 kb on - strand, within MMP_RS04875 at 927.314 kb on + strand, within MMP_RS04875 at 927.317 kb on - strand, within MMP_RS04875 at 927.318 kb on + strand, within MMP_RS04875 at 927.318 kb on + strand, within MMP_RS04875 at 927.352 kb on - strand at 927.353 kb on + strand at 927.367 kb on - strand at 927.367 kb on - strand at 927.368 kb on + strand at 927.368 kb on + strand at 927.368 kb on + strand at 927.369 kb on - strand at 927.369 kb on - strand at 927.369 kb on - strand at 927.370 kb on + strand at 927.370 kb on + strand at 927.370 kb on + strand at 927.386 kb on - strand at 927.392 kb on - strand at 927.393 kb on + strand at 927.397 kb on - strand at 927.398 kb on + strand at 927.398 kb on + strand at 927.398 kb on + strand at 927.411 kb on - strand at 927.411 kb on - strand at 927.411 kb on - strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.413 kb on - strand at 927.413 kb on - strand at 927.413 kb on - strand at 927.414 kb on + strand at 927.414 kb on + strand at 927.414 kb on + strand at 927.414 kb on + strand at 927.425 kb on - strand at 927.606 kb on + strand, within MMP_RS04880 at 927.675 kb on + strand, within MMP_RS04880 at 927.678 kb on + strand, within MMP_RS04880 at 927.695 kb on + strand, within MMP_RS04880 at 927.895 kb on - strand, within MMP_RS04880 at 927.899 kb on - strand, within MMP_RS04880 at 927.996 kb on - strand, within MMP_RS04880 at 927.996 kb on - strand, within MMP_RS04880 at 928.000 kb on - strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.112 kb on - strand, within MMP_RS04880 at 928.448 kb on + strand, within MMP_RS04880 at 928.452 kb on + strand, within MMP_RS04880 at 928.452 kb on + strand, within MMP_RS04880 at 928.452 kb on + strand, within MMP_RS04880 at 928.470 kb on + strand, within MMP_RS04880 at 928.519 kb on - strand, within MMP_RS04880 at 928.678 kb on - strand, within MMP_RS04880 at 928.730 kb on + strand, within MMP_RS04880 at 928.945 kb on - strand at 928.998 kb on + strand at 929.002 kb on + strand at 929.006 kb on - strand at 929.006 kb on - strand at 929.007 kb on + strand at 929.007 kb on + strand at 929.014 kb on - strand at 929.014 kb on - strand at 929.014 kb on - strand at 929.015 kb on + strand at 929.070 kb on + strand at 929.152 kb on + strand, within MMP_RS04885 at 929.169 kb on - strand, within MMP_RS04885 at 929.174 kb on + strand, within MMP_RS04885 at 929.176 kb on + strand, within MMP_RS04885 at 929.209 kb on + strand, within MMP_RS04885 at 929.261 kb on - strand, within MMP_RS04885 at 929.262 kb on + strand, within MMP_RS04885 at 929.315 kb on + strand, within MMP_RS04885 at 929.373 kb on + strand, within MMP_RS04885 at 929.373 kb on + strand, within MMP_RS04885 at 929.414 kb on + strand, within MMP_RS04885 at 929.447 kb on - strand, within MMP_RS04885 at 929.495 kb on - strand, within MMP_RS04885 at 929.500 kb on + strand, within MMP_RS04885 at 929.537 kb on + strand, within MMP_RS04885 at 929.565 kb on - strand, within MMP_RS04885 at 929.565 kb on - strand, within MMP_RS04885 at 929.576 kb on - strand, within MMP_RS04885 at 929.576 kb on - strand, within MMP_RS04885 at 929.602 kb on + strand, within MMP_RS04885 at 929.602 kb on + strand, within MMP_RS04885 at 929.667 kb on - strand, within MMP_RS04885 at 929.668 kb on + strand, within MMP_RS04885 at 929.701 kb on + strand, within MMP_RS04885 at 929.702 kb on - strand, within MMP_RS04885 at 929.790 kb on + strand at 929.794 kb on + strand at 929.813 kb on + strand at 929.817 kb on + strand at 929.817 kb on + strand at 929.817 kb on + strand at 929.844 kb on + strand at 929.859 kb on + strand at 929.909 kb on + strand, within MMP_RS04890 at 929.938 kb on + strand, within MMP_RS04890
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 926,431 - -3.6 926,432 + -3.9 926,440 + -4.0 926,440 + -3.9 926,440 + -3.2 926,517 + MMP_RS04870 0.20 -3.9 926,568 + MMP_RS04870 0.28 -4.3 926,589 + MMP_RS04870 0.31 -2.4 926,620 - MMP_RS04870 0.36 -3.3 926,621 + MMP_RS04870 0.36 -3.2 926,635 + MMP_RS04870 0.38 -4.8 926,636 - MMP_RS04870 0.38 -3.1 926,637 + MMP_RS04870 0.38 -2.5 926,808 + MMP_RS04870 0.63 -1.8 926,912 - MMP_RS04870 0.79 -3.3 927,073 - -2.8 927,073 - -3.2 927,073 - -1.1 927,074 + -2.9 927,076 + -4.5 927,083 + -2.1 927,118 + MMP_RS04875 0.14 -2.4 927,158 + MMP_RS04875 0.27 -2.1 927,294 + MMP_RS04875 0.75 -1.8 927,313 - MMP_RS04875 0.81 -1.0 927,314 + MMP_RS04875 0.82 -2.9 927,317 - MMP_RS04875 0.83 -1.2 927,318 + MMP_RS04875 0.83 -1.0 927,318 + MMP_RS04875 0.83 -1.9 927,352 - -1.5 927,353 + -2.1 927,367 - -2.4 927,367 - -0.6 927,368 + -2.5 927,368 + -3.5 927,368 + -2.4 927,369 - -0.8 927,369 - -2.0 927,369 - -1.3 927,370 + -2.1 927,370 + -2.1 927,370 + -1.8 927,386 - -0.6 927,392 - -0.1 927,393 + -2.4 927,397 - -2.3 927,398 + -1.9 927,398 + -1.7 927,398 + -2.0 927,411 - +0.3 927,411 - +0.7 927,411 - +0.5 927,412 + -0.3 927,412 + +0.7 927,412 + +3.6 927,412 + -0.9 927,412 + +0.5 927,412 + +0.1 927,413 - +0.3 927,413 - +0.6 927,413 - +0.1 927,414 + +0.3 927,414 + -0.1 927,414 + -0.5 927,414 + +0.2 927,425 - +0.6 927,606 + MMP_RS04880 0.12 -0.2 927,675 + MMP_RS04880 0.16 +0.3 927,678 + MMP_RS04880 0.16 +0.6 927,695 + MMP_RS04880 0.17 +0.4 927,895 - MMP_RS04880 0.31 -0.2 927,899 - MMP_RS04880 0.31 +0.2 927,996 - MMP_RS04880 0.37 -0.1 927,996 - MMP_RS04880 0.37 +0.2 928,000 - MMP_RS04880 0.38 +0.2 928,001 + MMP_RS04880 0.38 +0.8 928,001 + MMP_RS04880 0.38 +0.8 928,001 + MMP_RS04880 0.38 +0.5 928,001 + MMP_RS04880 0.38 +0.1 928,001 + MMP_RS04880 0.38 -0.0 928,112 - MMP_RS04880 0.45 -0.0 928,448 + MMP_RS04880 0.67 -0.1 928,452 + MMP_RS04880 0.68 +0.4 928,452 + MMP_RS04880 0.68 -0.3 928,452 + MMP_RS04880 0.68 +0.1 928,470 + MMP_RS04880 0.69 +0.1 928,519 - MMP_RS04880 0.72 -0.2 928,678 - MMP_RS04880 0.82 +4.4 928,730 + MMP_RS04880 0.86 +0.3 928,945 - -0.3 928,998 + -0.2 929,002 + +0.1 929,006 - +0.3 929,006 - -0.0 929,007 + +0.3 929,007 + -1.5 929,014 - -0.2 929,014 - +0.3 929,014 - -0.8 929,015 + +0.2 929,070 + +0.3 929,152 + MMP_RS04885 0.19 +0.1 929,169 - MMP_RS04885 0.21 +0.1 929,174 + MMP_RS04885 0.21 +0.1 929,176 + MMP_RS04885 0.22 +0.3 929,209 + MMP_RS04885 0.26 +0.5 929,261 - MMP_RS04885 0.32 -0.4 929,262 + MMP_RS04885 0.32 -0.7 929,315 + MMP_RS04885 0.39 +0.1 929,373 + MMP_RS04885 0.46 +0.5 929,373 + MMP_RS04885 0.46 +0.8 929,414 + MMP_RS04885 0.51 +0.3 929,447 - MMP_RS04885 0.55 -0.7 929,495 - MMP_RS04885 0.61 +0.6 929,500 + MMP_RS04885 0.62 +0.3 929,537 + MMP_RS04885 0.66 -0.3 929,565 - MMP_RS04885 0.69 +0.7 929,565 - MMP_RS04885 0.69 +0.5 929,576 - MMP_RS04885 0.71 +0.0 929,576 - MMP_RS04885 0.71 -0.3 929,602 + MMP_RS04885 0.74 -0.4 929,602 + MMP_RS04885 0.74 +0.3 929,667 - MMP_RS04885 0.82 +0.4 929,668 + MMP_RS04885 0.82 +0.6 929,701 + MMP_RS04885 0.86 -0.3 929,702 - MMP_RS04885 0.86 +0.9 929,790 + +0.3 929,794 + -0.2 929,813 + +0.6 929,817 + -0.4 929,817 + +1.1 929,817 + +0.7 929,844 + -5.5 929,859 + -3.3 929,909 + MMP_RS04890 0.18 -3.2 929,938 + MMP_RS04890 0.25 -3.7
Or see this region's nucleotide sequence