Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04440 and MMP_RS04445 are separated by 42 nucleotides MMP_RS04445 and MMP_RS04450 are separated by 9 nucleotides MMP_RS04450 and MMP_RS04455 are separated by 49 nucleotides
MMP_RS04440: MMP_RS04440 - nitrogenase iron protein, at 844,090 to 844,917
_RS04440
MMP_RS04445: MMP_RS04445 - P-II family nitrogen regulator, at 844,960 to 845,277
_RS04445
MMP_RS04450: MMP_RS04450 - P-II family nitrogen regulator, at 845,287 to 845,652
_RS04450
MMP_RS04455: MMP_RS04455 - nitrogenase subunit alpha, at 845,702 to 847,135
_RS04455
Position (kb)
844
845
846 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 843.997 kb on - strand at 843.998 kb on + strand at 843.998 kb on + strand at 843.998 kb on + strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.072 kb on - strand at 844.073 kb on + strand at 844.073 kb on + strand at 844.073 kb on + strand at 844.073 kb on + strand at 844.074 kb on - strand at 844.074 kb on - strand at 844.074 kb on - strand at 844.075 kb on + strand at 844.075 kb on + strand at 844.076 kb on - strand at 844.076 kb on - strand at 844.076 kb on - strand at 844.077 kb on + strand at 844.077 kb on + strand at 844.112 kb on + strand at 844.225 kb on + strand, within MMP_RS04440 at 844.256 kb on - strand, within MMP_RS04440 at 844.256 kb on - strand, within MMP_RS04440 at 844.257 kb on + strand, within MMP_RS04440 at 844.269 kb on - strand, within MMP_RS04440 at 844.269 kb on - strand, within MMP_RS04440 at 844.300 kb on + strand, within MMP_RS04440 at 844.337 kb on - strand, within MMP_RS04440 at 844.337 kb on - strand, within MMP_RS04440 at 844.481 kb on - strand, within MMP_RS04440 at 844.532 kb on + strand, within MMP_RS04440 at 844.655 kb on - strand, within MMP_RS04440 at 844.673 kb on - strand, within MMP_RS04440 at 844.808 kb on + strand, within MMP_RS04440 at 844.880 kb on - strand at 844.889 kb on - strand at 844.910 kb on - strand at 844.911 kb on + strand at 844.919 kb on - strand at 844.930 kb on + strand at 844.930 kb on + strand at 844.933 kb on - strand at 844.934 kb on + strand at 845.009 kb on - strand, within MMP_RS04445 at 845.086 kb on + strand, within MMP_RS04445 at 845.086 kb on + strand, within MMP_RS04445 at 845.105 kb on + strand, within MMP_RS04445 at 845.139 kb on + strand, within MMP_RS04445 at 845.165 kb on - strand, within MMP_RS04445 at 845.191 kb on - strand, within MMP_RS04445 at 845.191 kb on - strand, within MMP_RS04445 at 845.214 kb on - strand, within MMP_RS04445 at 845.245 kb on - strand, within MMP_RS04445 at 845.273 kb on - strand at 845.276 kb on + strand at 845.284 kb on - strand at 845.284 kb on - strand at 845.287 kb on + strand at 845.453 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.476 kb on - strand, within MMP_RS04450 at 845.477 kb on + strand, within MMP_RS04450 at 845.501 kb on - strand, within MMP_RS04450 at 845.502 kb on + strand, within MMP_RS04450 at 845.502 kb on + strand, within MMP_RS04450 at 845.502 kb on + strand, within MMP_RS04450 at 845.531 kb on - strand, within MMP_RS04450 at 845.531 kb on - strand, within MMP_RS04450 at 845.545 kb on + strand, within MMP_RS04450 at 845.552 kb on - strand, within MMP_RS04450 at 845.553 kb on + strand, within MMP_RS04450 at 845.557 kb on + strand, within MMP_RS04450 at 845.592 kb on + strand, within MMP_RS04450 at 845.759 kb on - strand at 845.760 kb on + strand at 845.807 kb on + strand at 845.807 kb on + strand at 845.807 kb on + strand at 845.830 kb on - strand at 845.830 kb on - strand at 845.831 kb on + strand at 845.886 kb on - strand, within MMP_RS04455 at 845.895 kb on - strand, within MMP_RS04455 at 845.895 kb on - strand, within MMP_RS04455 at 845.896 kb on + strand, within MMP_RS04455 at 845.896 kb on + strand, within MMP_RS04455 at 845.905 kb on + strand, within MMP_RS04455 at 845.907 kb on - strand, within MMP_RS04455 at 845.926 kb on - strand, within MMP_RS04455 at 845.927 kb on + strand, within MMP_RS04455 at 845.992 kb on + strand, within MMP_RS04455 at 846.040 kb on + strand, within MMP_RS04455 at 846.040 kb on + strand, within MMP_RS04455 at 846.131 kb on - strand, within MMP_RS04455 at 846.131 kb on - strand, within MMP_RS04455 at 846.132 kb on + strand, within MMP_RS04455 at 846.148 kb on - strand, within MMP_RS04455 at 846.188 kb on + strand, within MMP_RS04455
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 843,997 - -0.3 843,998 + +0.4 843,998 + +0.1 843,998 + +0.0 844,072 - -1.8 844,072 - -0.5 844,072 - +0.1 844,072 - +0.3 844,072 - -0.1 844,073 + +0.4 844,073 + +0.0 844,073 + -0.4 844,073 + +0.7 844,074 - +0.1 844,074 - +0.2 844,074 - +3.9 844,075 + +0.1 844,075 + -0.3 844,076 - +0.3 844,076 - -0.1 844,076 - -0.9 844,077 + +0.2 844,077 + -0.3 844,112 + -0.1 844,225 + MMP_RS04440 0.16 +0.6 844,256 - MMP_RS04440 0.20 +0.3 844,256 - MMP_RS04440 0.20 +0.2 844,257 + MMP_RS04440 0.20 -1.0 844,269 - MMP_RS04440 0.22 -0.5 844,269 - MMP_RS04440 0.22 -0.6 844,300 + MMP_RS04440 0.25 -0.3 844,337 - MMP_RS04440 0.30 -0.5 844,337 - MMP_RS04440 0.30 -0.1 844,481 - MMP_RS04440 0.47 +0.5 844,532 + MMP_RS04440 0.53 +0.0 844,655 - MMP_RS04440 0.68 -0.3 844,673 - MMP_RS04440 0.70 +0.3 844,808 + MMP_RS04440 0.87 +0.1 844,880 - -0.3 844,889 - -0.3 844,910 - +0.6 844,911 + +0.1 844,919 - +0.1 844,930 + +0.4 844,930 + -0.7 844,933 - +0.3 844,934 + -0.7 845,009 - MMP_RS04445 0.15 +0.3 845,086 + MMP_RS04445 0.40 -0.1 845,086 + MMP_RS04445 0.40 +3.7 845,105 + MMP_RS04445 0.46 +0.4 845,139 + MMP_RS04445 0.56 +0.3 845,165 - MMP_RS04445 0.64 +0.6 845,191 - MMP_RS04445 0.73 -0.1 845,191 - MMP_RS04445 0.73 +0.2 845,214 - MMP_RS04445 0.80 +0.1 845,245 - MMP_RS04445 0.90 +0.3 845,273 - +0.0 845,276 + -0.2 845,284 - -0.1 845,284 - -0.5 845,287 + -0.0 845,453 - MMP_RS04450 0.45 -0.8 845,476 - MMP_RS04450 0.52 -0.7 845,476 - MMP_RS04450 0.52 +0.1 845,476 - MMP_RS04450 0.52 -0.0 845,476 - MMP_RS04450 0.52 -0.1 845,476 - MMP_RS04450 0.52 -0.4 845,477 + MMP_RS04450 0.52 -0.1 845,501 - MMP_RS04450 0.58 -0.2 845,502 + MMP_RS04450 0.59 +0.3 845,502 + MMP_RS04450 0.59 +0.4 845,502 + MMP_RS04450 0.59 +0.1 845,531 - MMP_RS04450 0.67 -0.0 845,531 - MMP_RS04450 0.67 +0.2 845,545 + MMP_RS04450 0.70 +0.3 845,552 - MMP_RS04450 0.72 -0.6 845,553 + MMP_RS04450 0.73 +0.4 845,557 + MMP_RS04450 0.74 +0.1 845,592 + MMP_RS04450 0.83 +0.1 845,759 - +0.6 845,760 + +0.1 845,807 + -0.2 845,807 + +0.0 845,807 + +0.8 845,830 - +0.8 845,830 - +0.3 845,831 + -0.5 845,886 - MMP_RS04455 0.13 -0.8 845,895 - MMP_RS04455 0.13 -0.2 845,895 - MMP_RS04455 0.13 -0.0 845,896 + MMP_RS04455 0.14 -0.7 845,896 + MMP_RS04455 0.14 -0.5 845,905 + MMP_RS04455 0.14 -1.9 845,907 - MMP_RS04455 0.14 -0.2 845,926 - MMP_RS04455 0.16 -0.2 845,927 + MMP_RS04455 0.16 +0.5 845,992 + MMP_RS04455 0.20 -0.1 846,040 + MMP_RS04455 0.24 -0.9 846,040 + MMP_RS04455 0.24 +0.3 846,131 - MMP_RS04455 0.30 +0.2 846,131 - MMP_RS04455 0.30 -0.5 846,132 + MMP_RS04455 0.30 +0.4 846,148 - MMP_RS04455 0.31 -0.1 846,188 + MMP_RS04455 0.34 +0.6
Or see this region's nucleotide sequence