Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02975 and MMP_RS02980 are separated by 63 nucleotides MMP_RS02980 and MMP_RS02985 are separated by 19 nucleotides MMP_RS02985 and MMP_RS02990 are separated by 41 nucleotides
MMP_RS02975: MMP_RS02975 - DUF2124 domain-containing protein, at 563,258 to 563,740
_RS02975
MMP_RS02980: MMP_RS02980 - ATP-grasp domain-containing protein, at 563,804 to 564,712
_RS02980
MMP_RS02985: MMP_RS02985 - hypothetical protein, at 564,732 to 565,655
_RS02985
MMP_RS02990: MMP_RS02990 - AMP-binding protein, at 565,697 to 567,346
_RS02990
Position (kb) 564
565
566 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 563.772 kb on + strand at 563.772 kb on + strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.887 kb on - strand at 564.120 kb on - strand at 564.213 kb on - strand at 564.422 kb on - strand at 564.495 kb on - strand at 564.495 kb on - strand at 564.512 kb on - strand at 564.537 kb on - strand at 564.553 kb on - strand at 564.569 kb on - strand at 564.569 kb on - strand at 564.588 kb on - strand at 564.680 kb on + strand at 564.696 kb on + strand at 564.748 kb on + strand at 564.972 kb on - strand, within MMP_RS02985 at 565.041 kb on + strand, within MMP_RS02985 at 565.269 kb on - strand, within MMP_RS02985 at 565.490 kb on - strand, within MMP_RS02985 at 565.568 kb on - strand at 565.603 kb on + strand at 565.609 kb on - strand at 565.646 kb on - strand at 565.649 kb on + strand at 565.649 kb on + strand at 565.649 kb on + strand at 565.649 kb on + strand at 565.668 kb on + strand at 565.692 kb on + strand at 565.693 kb on - strand at 565.694 kb on + strand at 565.695 kb on - strand at 565.819 kb on - strand at 565.819 kb on - strand at 565.867 kb on - strand, within MMP_RS02990 at 565.875 kb on - strand, within MMP_RS02990 at 565.972 kb on + strand, within MMP_RS02990 at 565.998 kb on - strand, within MMP_RS02990 at 566.345 kb on + strand, within MMP_RS02990 at 566.362 kb on + strand, within MMP_RS02990 at 566.362 kb on + strand, within MMP_RS02990 at 566.365 kb on - strand, within MMP_RS02990 at 566.376 kb on + strand, within MMP_RS02990 at 566.489 kb on + strand, within MMP_RS02990 at 566.500 kb on + strand, within MMP_RS02990 at 566.526 kb on + strand, within MMP_RS02990
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 563,772 + -0.3 563,772 + -0.5 563,776 - +1.2 563,776 - +0.3 563,887 - -3.7 564,120 - -4.2 564,213 - -3.9 564,422 - -2.5 564,495 - -4.5 564,495 - -3.3 564,512 - -3.5 564,537 - -5.5 564,553 - -2.3 564,569 - -3.5 564,569 - -4.9 564,588 - -3.0 564,680 + -3.7 564,696 + -2.4 564,748 + -0.0 564,972 - MMP_RS02985 0.26 +2.3 565,041 + MMP_RS02985 0.33 +0.4 565,269 - MMP_RS02985 0.58 +0.4 565,490 - MMP_RS02985 0.82 +0.3 565,568 - -1.0 565,603 + +0.3 565,609 - +0.9 565,646 - -0.1 565,649 + -0.2 565,649 + +0.4 565,649 + -0.2 565,649 + +0.7 565,668 + +0.4 565,692 + +0.1 565,693 - -0.0 565,694 + +0.4 565,695 - +1.2 565,819 - -0.2 565,819 - +0.2 565,867 - MMP_RS02990 0.10 +4.0 565,875 - MMP_RS02990 0.11 +0.7 565,972 + MMP_RS02990 0.17 -0.7 565,998 - MMP_RS02990 0.18 +2.2 566,345 + MMP_RS02990 0.39 +0.4 566,362 + MMP_RS02990 0.40 +0.7 566,362 + MMP_RS02990 0.40 +0.3 566,365 - MMP_RS02990 0.40 +0.2 566,376 + MMP_RS02990 0.41 +0.2 566,489 + MMP_RS02990 0.48 +0.3 566,500 + MMP_RS02990 0.49 +0.4 566,526 + MMP_RS02990 0.50 +0.3
Or see this region's nucleotide sequence