Strain Fitness in Methanococcus maripaludis S2 around MMP_RS02495

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS02480 and MMP_RS02485 are separated by 2 nucleotidesMMP_RS02485 and MMP_RS02490 overlap by 14 nucleotidesMMP_RS02490 and MMP_RS02495 are separated by 11 nucleotidesMMP_RS02495 and MMP_RS02500 are separated by 1 nucleotides MMP_RS02480: MMP_RS02480 - hypothetical protein, at 464,479 to 464,997 _RS02480 MMP_RS02485: MMP_RS02485 - DUF2080 family transposase-associated protein, at 465,000 to 465,224 _RS02485 MMP_RS02490: MMP_RS02490 - hypothetical protein, at 465,211 to 465,405 _RS02490 MMP_RS02495: MMP_RS02495 - hypothetical protein, at 465,417 to 465,800 _RS02495 MMP_RS02500: MMP_RS02500 - minichromosome maintenance protein MCM, at 465,802 to 467,826 _RS02500 Position (kb) 465 466Strain fitness (log2 ratio) -1 0 1at 464.455 kb on - strandat 464.676 kb on + strand, within MMP_RS02480at 464.825 kb on - strand, within MMP_RS02480at 465.306 kb on - strand, within MMP_RS02490at 465.306 kb on - strand, within MMP_RS02490at 465.307 kb on + strand, within MMP_RS02490at 465.432 kb on - strandat 465.679 kb on + strand, within MMP_RS02495at 465.682 kb on - strand, within MMP_RS02495at 465.851 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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464,455 - +0.4
464,676 + MMP_RS02480 0.38 -0.0
464,825 - MMP_RS02480 0.67 -0.1
465,306 - MMP_RS02490 0.49 -0.2
465,306 - MMP_RS02490 0.49 -0.6
465,307 + MMP_RS02490 0.49 +0.3
465,432 - +0.4
465,679 + MMP_RS02495 0.68 +0.5
465,682 - MMP_RS02495 0.69 +0.3
465,851 - +0.5

Or see this region's nucleotide sequence