Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02015 and MMP_RS02020 are separated by 5 nucleotides MMP_RS02020 and MMP_RS02025 are separated by 4 nucleotides MMP_RS02025 and MMP_RS02030 are separated by 179 nucleotides MMP_RS02030 and MMP_RS02035 are separated by 78 nucleotides
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
MMP_RS02020: MMP_RS02020 - gamma-glutamylcyclotransferase, at 376,162 to 376,485
_RS02020
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
MMP_RS02030: MMP_RS02030 - homoaconitase small subunit, at 379,024 to 379,512
_RS02030
MMP_RS02035: MMP_RS02035 - ribosome biogenesis/translation initiation ATPase RLI, at 379,591 to 381,363
_RS02035
Position (kb) 376
377
378
379 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 375.518 kb on - strand, within MMP_RS02015 at 375.626 kb on + strand, within MMP_RS02015 at 375.665 kb on - strand, within MMP_RS02015 at 375.667 kb on - strand, within MMP_RS02015 at 375.835 kb on + strand, within MMP_RS02015 at 376.049 kb on + strand at 376.127 kb on + strand at 376.131 kb on - strand at 376.132 kb on + strand at 376.179 kb on + strand at 376.221 kb on - strand, within MMP_RS02020 at 376.300 kb on - strand, within MMP_RS02020 at 376.318 kb on + strand, within MMP_RS02020 at 376.385 kb on - strand, within MMP_RS02020 at 376.479 kb on + strand at 376.568 kb on - strand at 376.679 kb on - strand at 376.682 kb on + strand at 376.682 kb on + strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.730 kb on - strand, within MMP_RS02025 at 376.768 kb on - strand, within MMP_RS02025 at 376.789 kb on - strand, within MMP_RS02025 at 376.974 kb on + strand, within MMP_RS02025 at 377.239 kb on + strand, within MMP_RS02025 at 377.542 kb on - strand, within MMP_RS02025 at 377.543 kb on + strand, within MMP_RS02025 at 377.550 kb on + strand, within MMP_RS02025 at 377.564 kb on - strand, within MMP_RS02025 at 377.582 kb on + strand, within MMP_RS02025 at 377.651 kb on - strand, within MMP_RS02025 at 377.810 kb on - strand, within MMP_RS02025 at 377.869 kb on + strand, within MMP_RS02025 at 377.872 kb on + strand, within MMP_RS02025 at 377.909 kb on - strand, within MMP_RS02025 at 378.014 kb on - strand, within MMP_RS02025 at 378.064 kb on + strand, within MMP_RS02025 at 378.261 kb on - strand, within MMP_RS02025 at 378.418 kb on - strand, within MMP_RS02025 at 378.473 kb on + strand, within MMP_RS02025 at 378.506 kb on + strand, within MMP_RS02025 at 378.540 kb on + strand, within MMP_RS02025 at 378.770 kb on - strand at 378.771 kb on + strand at 378.771 kb on + strand at 378.782 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.791 kb on - strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.792 kb on + strand at 378.795 kb on - strand at 378.796 kb on + strand at 378.796 kb on + strand at 378.925 kb on - strand at 378.964 kb on - strand at 379.190 kb on - strand at 379.380 kb on + strand at 379.550 kb on + strand at 379.557 kb on + strand at 379.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 375,518 - MMP_RS02015 0.50 +0.5 375,626 + MMP_RS02015 0.59 +0.4 375,665 - MMP_RS02015 0.62 -0.1 375,667 - MMP_RS02015 0.62 +0.1 375,835 + MMP_RS02015 0.75 +0.3 376,049 + +0.4 376,127 + +0.1 376,131 - -0.4 376,132 + -0.6 376,179 + -0.0 376,221 - MMP_RS02020 0.18 +0.5 376,300 - MMP_RS02020 0.43 -0.0 376,318 + MMP_RS02020 0.48 -0.1 376,385 - MMP_RS02020 0.69 +0.1 376,479 + +0.1 376,568 - +0.5 376,679 - +0.4 376,682 + +0.5 376,682 + -1.1 376,691 - +0.5 376,691 - +0.2 376,691 - -0.0 376,730 - MMP_RS02025 0.10 -0.5 376,768 - MMP_RS02025 0.12 +0.4 376,789 - MMP_RS02025 0.13 +0.0 376,974 + MMP_RS02025 0.21 +0.7 377,239 + MMP_RS02025 0.32 +0.5 377,542 - MMP_RS02025 0.45 +0.8 377,543 + MMP_RS02025 0.45 +0.4 377,550 + MMP_RS02025 0.45 +0.6 377,564 - MMP_RS02025 0.46 +0.5 377,582 + MMP_RS02025 0.46 +4.0 377,651 - MMP_RS02025 0.49 +0.5 377,810 - MMP_RS02025 0.56 +0.5 377,869 + MMP_RS02025 0.59 -0.1 377,872 + MMP_RS02025 0.59 +0.1 377,909 - MMP_RS02025 0.60 +0.8 378,014 - MMP_RS02025 0.65 +0.6 378,064 + MMP_RS02025 0.67 -0.2 378,261 - MMP_RS02025 0.75 +0.6 378,418 - MMP_RS02025 0.82 +0.5 378,473 + MMP_RS02025 0.84 +0.5 378,506 + MMP_RS02025 0.86 -0.2 378,540 + MMP_RS02025 0.87 -0.5 378,770 - +0.0 378,771 + +1.1 378,771 + -0.0 378,782 - +0.7 378,791 - +0.1 378,791 - +0.7 378,791 - +0.7 378,792 + +0.5 378,792 + -0.4 378,792 + -0.1 378,795 - +0.9 378,796 + +0.3 378,796 + -0.1 378,925 - -2.7 378,964 - -4.0 379,190 - -3.1 379,380 + -3.1 379,550 + -0.1 379,557 + +0.1 379,615 - -0.5
Or see this region's nucleotide sequence