Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01985 and MMP_RS01990 are separated by 203 nucleotides MMP_RS01990 and MMP_RS01995 are separated by 39 nucleotides MMP_RS01995 and MMP_RS02000 are separated by 84 nucleotides
MMP_RS01985: MMP_RS01985 - F420-dependent methylenetetrahydromethanopterin dehydrogenase, at 369,232 to 370,065
_RS01985
MMP_RS01990: MMP_RS01990 - hypothetical protein, at 370,269 to 370,517
_RS01990
MMP_RS01995: MMP_RS01995 - ribonuclease VapC5, at 370,557 to 371,087
_RS01995
MMP_RS02000: MMP_RS02000 - radical SAM protein, at 371,172 to 372,545
_RS02000
Position (kb)
370
371 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 369.361 kb on - strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.683 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 370.067 kb on + strand at 370.067 kb on + strand at 370.067 kb on + strand at 370.100 kb on - strand at 370.120 kb on - strand at 370.121 kb on + strand at 370.178 kb on - strand at 370.202 kb on + strand at 370.202 kb on + strand at 370.215 kb on - strand at 370.223 kb on - strand at 370.318 kb on - strand, within MMP_RS01990 at 370.318 kb on - strand, within MMP_RS01990 at 370.344 kb on + strand, within MMP_RS01990 at 370.382 kb on + strand, within MMP_RS01990 at 370.516 kb on + strand at 370.554 kb on - strand at 370.555 kb on + strand at 370.555 kb on + strand at 370.591 kb on - strand at 370.592 kb on + strand at 370.811 kb on - strand at 371.087 kb on - strand at 371.104 kb on - strand at 371.104 kb on - strand at 371.105 kb on + strand at 371.108 kb on - strand at 371.109 kb on + strand at 371.113 kb on + strand at 371.114 kb on - strand at 371.115 kb on + strand at 371.115 kb on + strand at 371.118 kb on - strand at 371.119 kb on + strand at 371.119 kb on + strand at 371.119 kb on + strand at 371.120 kb on - strand at 371.123 kb on - strand at 371.123 kb on - strand at 371.123 kb on - strand at 371.123 kb on - strand at 371.124 kb on + strand at 371.124 kb on + strand at 371.124 kb on + strand at 371.124 kb on + strand at 371.125 kb on - strand at 371.126 kb on + strand at 371.132 kb on + strand at 371.145 kb on + strand at 371.148 kb on - strand at 371.149 kb on + strand at 371.490 kb on - strand, within MMP_RS02000 at 371.494 kb on + strand, within MMP_RS02000
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 369,361 - MMP_RS01985 0.15 -3.5 369,647 + MMP_RS01985 0.50 -3.4 369,647 + MMP_RS01985 0.50 -3.0 369,683 + MMP_RS01985 0.54 -2.6 369,890 + MMP_RS01985 0.79 -2.4 369,890 + MMP_RS01985 0.79 -3.5 370,067 + -3.1 370,067 + -2.4 370,067 + -3.1 370,100 - -3.0 370,120 - -3.6 370,121 + -4.1 370,178 - -0.4 370,202 + +0.1 370,202 + +0.3 370,215 - -0.2 370,223 - -0.2 370,318 - MMP_RS01990 0.20 +0.5 370,318 - MMP_RS01990 0.20 -0.4 370,344 + MMP_RS01990 0.30 +0.6 370,382 + MMP_RS01990 0.45 +0.6 370,516 + +0.4 370,554 - +0.3 370,555 + +0.0 370,555 + +0.0 370,591 - +0.1 370,592 + +0.0 370,811 - -3.4 371,087 - -3.7 371,104 - +0.3 371,104 - -0.2 371,105 + +0.2 371,108 - +0.1 371,109 + +0.8 371,113 + +0.8 371,114 - +0.2 371,115 + -0.1 371,115 + +0.2 371,118 - +0.1 371,119 + -0.3 371,119 + +0.2 371,119 + -0.8 371,120 - +0.5 371,123 - -1.1 371,123 - +0.3 371,123 - -0.4 371,123 - +0.4 371,124 + +0.1 371,124 + +0.0 371,124 + -0.1 371,124 + +0.5 371,125 - +0.3 371,126 + +0.1 371,132 + +0.4 371,145 + +0.3 371,148 - -0.3 371,149 + -0.2 371,490 - MMP_RS02000 0.23 +0.4 371,494 + MMP_RS02000 0.23 -1.1
Or see this region's nucleotide sequence