Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01935 and MMP_RS01940 are separated by 3 nucleotides MMP_RS01940 and MMP_RS01945 overlap by 26 nucleotides MMP_RS01945 and MMP_RS01950 overlap by 1 nucleotides
MMP_RS01935: MMP_RS01935 - DUF58 domain-containing protein, at 358,253 to 359,554
_RS01935
MMP_RS01940: MMP_RS01940 - MoxR family ATPase, at 359,558 to 360,499
_RS01940
MMP_RS01945: MMP_RS01945 - DUF4350 domain-containing protein, at 360,474 to 361,472
_RS01945
MMP_RS01950: MMP_RS01950 - hypothetical protein, at 361,472 to 363,307
_RS01950
Position (kb) 359
360
361 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 358.618 kb on - strand, within MMP_RS01935 at 358.621 kb on - strand, within MMP_RS01935 at 358.665 kb on + strand, within MMP_RS01935 at 358.670 kb on + strand, within MMP_RS01935 at 358.678 kb on - strand, within MMP_RS01935 at 358.679 kb on + strand, within MMP_RS01935 at 358.725 kb on + strand, within MMP_RS01935 at 358.725 kb on + strand, within MMP_RS01935 at 358.782 kb on - strand, within MMP_RS01935 at 358.783 kb on + strand, within MMP_RS01935 at 358.785 kb on - strand, within MMP_RS01935 at 358.785 kb on - strand, within MMP_RS01935 at 358.785 kb on - strand, within MMP_RS01935 at 358.786 kb on + strand, within MMP_RS01935 at 358.786 kb on + strand, within MMP_RS01935 at 358.787 kb on - strand, within MMP_RS01935 at 358.788 kb on + strand, within MMP_RS01935 at 358.811 kb on - strand, within MMP_RS01935 at 358.816 kb on + strand, within MMP_RS01935 at 358.816 kb on + strand, within MMP_RS01935 at 358.862 kb on + strand, within MMP_RS01935 at 358.864 kb on - strand, within MMP_RS01935 at 358.865 kb on + strand, within MMP_RS01935 at 358.957 kb on - strand, within MMP_RS01935 at 358.992 kb on + strand, within MMP_RS01935 at 358.992 kb on + strand, within MMP_RS01935 at 359.090 kb on + strand, within MMP_RS01935 at 359.117 kb on + strand, within MMP_RS01935 at 359.174 kb on - strand, within MMP_RS01935 at 359.174 kb on - strand, within MMP_RS01935 at 359.195 kb on - strand, within MMP_RS01935 at 359.445 kb on + strand at 359.445 kb on + strand at 359.446 kb on - strand at 359.446 kb on - strand at 359.446 kb on - strand at 359.447 kb on + strand at 359.483 kb on - strand at 359.483 kb on - strand at 359.486 kb on + strand at 359.529 kb on - strand at 359.529 kb on - strand at 359.529 kb on - strand at 359.591 kb on + strand at 359.662 kb on - strand, within MMP_RS01940 at 359.712 kb on - strand, within MMP_RS01940 at 359.789 kb on - strand, within MMP_RS01940 at 360.037 kb on + strand, within MMP_RS01940 at 360.099 kb on + strand, within MMP_RS01940 at 360.176 kb on + strand, within MMP_RS01940 at 360.180 kb on + strand, within MMP_RS01940 at 360.458 kb on - strand at 360.480 kb on + strand at 360.513 kb on - strand at 360.514 kb on + strand at 360.518 kb on - strand at 360.612 kb on + strand, within MMP_RS01945 at 360.649 kb on + strand, within MMP_RS01945 at 360.743 kb on - strand, within MMP_RS01945 at 360.884 kb on - strand, within MMP_RS01945 at 360.885 kb on + strand, within MMP_RS01945 at 360.901 kb on - strand, within MMP_RS01945 at 360.906 kb on - strand, within MMP_RS01945 at 360.907 kb on + strand, within MMP_RS01945 at 360.992 kb on + strand, within MMP_RS01945 at 360.995 kb on - strand, within MMP_RS01945 at 360.996 kb on + strand, within MMP_RS01945 at 361.013 kb on - strand, within MMP_RS01945 at 361.015 kb on - strand, within MMP_RS01945 at 361.045 kb on - strand, within MMP_RS01945 at 361.274 kb on + strand, within MMP_RS01945 at 361.282 kb on - strand, within MMP_RS01945 at 361.291 kb on - strand, within MMP_RS01945 at 361.292 kb on + strand, within MMP_RS01945 at 361.294 kb on + strand, within MMP_RS01945 at 361.294 kb on + strand, within MMP_RS01945
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 358,618 - MMP_RS01935 0.28 -2.3 358,621 - MMP_RS01935 0.28 -2.1 358,665 + MMP_RS01935 0.32 -0.4 358,670 + MMP_RS01935 0.32 -2.6 358,678 - MMP_RS01935 0.33 -2.9 358,679 + MMP_RS01935 0.33 -2.3 358,725 + MMP_RS01935 0.36 -1.6 358,725 + MMP_RS01935 0.36 -1.0 358,782 - MMP_RS01935 0.41 -2.8 358,783 + MMP_RS01935 0.41 -3.7 358,785 - MMP_RS01935 0.41 -2.7 358,785 - MMP_RS01935 0.41 -1.4 358,785 - MMP_RS01935 0.41 -1.8 358,786 + MMP_RS01935 0.41 -3.4 358,786 + MMP_RS01935 0.41 -2.9 358,787 - MMP_RS01935 0.41 -1.7 358,788 + MMP_RS01935 0.41 -2.4 358,811 - MMP_RS01935 0.43 -1.6 358,816 + MMP_RS01935 0.43 -2.1 358,816 + MMP_RS01935 0.43 -1.6 358,862 + MMP_RS01935 0.47 -0.9 358,864 - MMP_RS01935 0.47 -1.9 358,865 + MMP_RS01935 0.47 -2.0 358,957 - MMP_RS01935 0.54 -2.6 358,992 + MMP_RS01935 0.57 -1.9 358,992 + MMP_RS01935 0.57 -1.3 359,090 + MMP_RS01935 0.64 -3.3 359,117 + MMP_RS01935 0.66 -1.6 359,174 - MMP_RS01935 0.71 -2.0 359,174 - MMP_RS01935 0.71 -3.5 359,195 - MMP_RS01935 0.72 -2.7 359,445 + -1.5 359,445 + -2.1 359,446 - -3.0 359,446 - -0.9 359,446 - -2.5 359,447 + -1.3 359,483 - -1.8 359,483 - -2.2 359,486 + -1.9 359,529 - -3.0 359,529 - -2.6 359,529 - -2.3 359,591 + -2.0 359,662 - MMP_RS01940 0.11 -1.9 359,712 - MMP_RS01940 0.16 -1.6 359,789 - MMP_RS01940 0.25 -1.3 360,037 + MMP_RS01940 0.51 -1.3 360,099 + MMP_RS01940 0.57 -2.5 360,176 + MMP_RS01940 0.66 -3.0 360,180 + MMP_RS01940 0.66 -4.0 360,458 - -2.3 360,480 + -2.9 360,513 - -2.7 360,514 + -2.0 360,518 - -1.8 360,612 + MMP_RS01945 0.14 -3.2 360,649 + MMP_RS01945 0.18 -2.0 360,743 - MMP_RS01945 0.27 -3.5 360,884 - MMP_RS01945 0.41 -1.4 360,885 + MMP_RS01945 0.41 -1.6 360,901 - MMP_RS01945 0.43 -3.2 360,906 - MMP_RS01945 0.43 -2.1 360,907 + MMP_RS01945 0.43 -2.9 360,992 + MMP_RS01945 0.52 -2.8 360,995 - MMP_RS01945 0.52 -0.6 360,996 + MMP_RS01945 0.52 +0.5 361,013 - MMP_RS01945 0.54 -3.8 361,015 - MMP_RS01945 0.54 -1.8 361,045 - MMP_RS01945 0.57 -2.1 361,274 + MMP_RS01945 0.80 -2.2 361,282 - MMP_RS01945 0.81 -2.2 361,291 - MMP_RS01945 0.82 -2.5 361,292 + MMP_RS01945 0.82 -2.1 361,294 + MMP_RS01945 0.82 -0.1 361,294 + MMP_RS01945 0.82 -3.5
Or see this region's nucleotide sequence