Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01455 and MMP_RS01460 are separated by 23 nucleotides MMP_RS01460 and MMP_RS01465 are separated by 1 nucleotides MMP_RS01465 and MMP_RS01470 are separated by 25 nucleotides MMP_RS01470 and MMP_RS01475 are separated by 18 nucleotides
MMP_RS01455: MMP_RS01455 - nickel pincer cofactor biosynthesis protein LarC, at 268,844 to 270,058
_RS01455
MMP_RS01460: MMP_RS01460 - hypothetical protein, at 270,082 to 270,741
_RS01460
MMP_RS01465: MMP_RS01465 - hypothetical protein, at 270,743 to 271,441
_RS01465
MMP_RS01470: MMP_RS01470 - ATP-binding cassette domain-containing protein, at 271,467 to 272,258
_RS01470
MMP_RS01475: MMP_RS01475 - phosphosulfolactate synthase, at 272,277 to 273,032
_RS01475
Position (kb) 270
271
272 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 269.824 kb on - strand, within MMP_RS01455 at 270.061 kb on + strand at 270.061 kb on + strand at 270.064 kb on - strand at 270.069 kb on - strand at 270.069 kb on - strand at 270.070 kb on + strand at 270.070 kb on + strand at 270.071 kb on - strand at 270.071 kb on - strand at 270.072 kb on + strand at 270.075 kb on + strand at 270.078 kb on - strand at 270.079 kb on + strand at 270.084 kb on + strand at 270.088 kb on - strand at 270.224 kb on - strand, within MMP_RS01460 at 270.224 kb on - strand, within MMP_RS01460 at 270.324 kb on + strand, within MMP_RS01460 at 270.756 kb on - strand at 271.001 kb on - strand, within MMP_RS01465 at 271.023 kb on + strand, within MMP_RS01465 at 271.037 kb on + strand, within MMP_RS01465 at 271.396 kb on - strand at 271.686 kb on - strand, within MMP_RS01470 at 271.767 kb on - strand, within MMP_RS01470 at 271.839 kb on - strand, within MMP_RS01470 at 271.890 kb on - strand, within MMP_RS01470 at 271.974 kb on + strand, within MMP_RS01470 at 272.187 kb on - strand at 272.382 kb on - strand, within MMP_RS01475 at 272.388 kb on + strand, within MMP_RS01475 at 272.427 kb on - strand, within MMP_RS01475 at 272.428 kb on + strand, within MMP_RS01475 at 272.428 kb on + strand, within MMP_RS01475
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 269,824 - MMP_RS01455 0.81 -7.0 270,061 + +0.5 270,061 + +0.2 270,064 - -0.2 270,069 - -0.2 270,069 - +0.8 270,070 + +4.1 270,070 + +0.2 270,071 - +0.7 270,071 - -0.2 270,072 + -0.2 270,075 + +0.6 270,078 - +0.1 270,079 + +0.8 270,084 + -0.3 270,088 - +0.1 270,224 - MMP_RS01460 0.22 -0.3 270,224 - MMP_RS01460 0.22 +0.8 270,324 + MMP_RS01460 0.37 +0.1 270,756 - +0.5 271,001 - MMP_RS01465 0.37 +0.3 271,023 + MMP_RS01465 0.40 +0.7 271,037 + MMP_RS01465 0.42 +0.1 271,396 - -0.4 271,686 - MMP_RS01470 0.28 -0.4 271,767 - MMP_RS01470 0.38 -0.1 271,839 - MMP_RS01470 0.47 +0.2 271,890 - MMP_RS01470 0.53 +0.2 271,974 + MMP_RS01470 0.64 +0.6 272,187 - +0.4 272,382 - MMP_RS01475 0.14 -0.1 272,388 + MMP_RS01475 0.15 +0.4 272,427 - MMP_RS01475 0.20 -0.6 272,428 + MMP_RS01475 0.20 -0.6 272,428 + MMP_RS01475 0.20 -0.2
Or see this region's nucleotide sequence