Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01240

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01235 and MMP_RS01240 are separated by 15 nucleotidesMMP_RS01240 and MMP_RS01245 are separated by 131 nucleotides MMP_RS01235: MMP_RS01235 - iron-containing alcohol dehydrogenase, at 232,456 to 233,460 _RS01235 MMP_RS01240: MMP_RS01240 - 7-cyano-7-deazaguanine synthase QueC, at 233,476 to 234,177 _RS01240 MMP_RS01245: MMP_RS01245 - anaerobic ribonucleoside-triphosphate reductase, at 234,309 to 236,621 _RS01245 Position (kb) 233 234 235Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 232.738 kb on + strandat 233.312 kb on - strandat 233.340 kb on + strandat 234.087 kb on - strand, within MMP_RS01240at 234.094 kb on + strand, within MMP_RS01240at 234.156 kb on - strandat 234.214 kb on + strandat 234.285 kb on + strandat 234.333 kb on + strandat 234.509 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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232,738 + -2.5
233,312 - -0.9
233,340 + -3.7
234,087 - MMP_RS01240 0.87 +1.6
234,094 + MMP_RS01240 0.88 -3.1
234,156 - +0.6
234,214 + -3.3
234,285 + +0.0
234,333 + -0.1
234,509 + -3.9

Or see this region's nucleotide sequence