Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00535 and MMP_RS00540 are separated by 2 nucleotides MMP_RS00540 and MMP_RS00545 are separated by 124 nucleotides MMP_RS00545 and MMP_RS00550 are separated by 30 nucleotides
MMP_RS00535: MMP_RS00535 - TIGR00375 family protein, at 104,708 to 105,892
_RS00535
MMP_RS00540: MMP_RS00540 - PLP-dependent aminotransferase family protein, at 105,895 to 107,082
_RS00540
MMP_RS00545: MMP_RS00545 - XRE family transcriptional regulator, at 107,207 to 107,755
_RS00545
MMP_RS00550: MMP_RS00550 - 4Fe-4S binding protein, at 107,786 to 107,983
_RS00550
Position (kb)
105
106
107
108 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 104.947 kb on - strand, within MMP_RS00535 at 104.947 kb on - strand, within MMP_RS00535 at 105.791 kb on - strand at 106.134 kb on - strand, within MMP_RS00540 at 106.192 kb on + strand, within MMP_RS00540 at 106.193 kb on - strand, within MMP_RS00540 at 106.196 kb on + strand, within MMP_RS00540 at 106.300 kb on - strand, within MMP_RS00540 at 106.300 kb on - strand, within MMP_RS00540 at 106.302 kb on - strand, within MMP_RS00540 at 106.706 kb on - strand, within MMP_RS00540 at 106.706 kb on - strand, within MMP_RS00540 at 106.724 kb on + strand, within MMP_RS00540 at 106.997 kb on + strand at 107.145 kb on - strand at 107.148 kb on + strand at 107.155 kb on + strand at 107.167 kb on + strand at 107.178 kb on + strand at 107.181 kb on - strand at 107.181 kb on - strand at 107.181 kb on - strand at 107.182 kb on + strand at 107.186 kb on - strand at 107.218 kb on - strand at 107.219 kb on + strand at 107.292 kb on - strand, within MMP_RS00545 at 107.292 kb on - strand, within MMP_RS00545 at 107.529 kb on + strand, within MMP_RS00545 at 107.559 kb on + strand, within MMP_RS00545 at 107.597 kb on + strand, within MMP_RS00545 at 107.974 kb on - strand at 107.975 kb on + strand at 107.979 kb on + strand at 107.998 kb on - strand at 108.033 kb on + strand at 108.052 kb on - strand at 108.062 kb on - strand at 108.063 kb on + strand at 108.076 kb on - strand at 108.076 kb on - strand at 108.080 kb on - strand at 108.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 104,947 - MMP_RS00535 0.20 +0.1 104,947 - MMP_RS00535 0.20 -0.3 105,791 - +1.0 106,134 - MMP_RS00540 0.20 +0.3 106,192 + MMP_RS00540 0.25 +1.0 106,193 - MMP_RS00540 0.25 +0.3 106,196 + MMP_RS00540 0.25 +0.1 106,300 - MMP_RS00540 0.34 +3.9 106,300 - MMP_RS00540 0.34 +0.1 106,302 - MMP_RS00540 0.34 -0.5 106,706 - MMP_RS00540 0.68 +0.0 106,706 - MMP_RS00540 0.68 +0.3 106,724 + MMP_RS00540 0.70 +0.7 106,997 + -0.4 107,145 - -0.3 107,148 + +0.4 107,155 + +0.5 107,167 + +0.6 107,178 + +0.6 107,181 - -0.3 107,181 - -0.0 107,181 - +0.2 107,182 + +0.7 107,186 - +0.1 107,218 - -0.2 107,219 + -0.1 107,292 - MMP_RS00545 0.15 +0.4 107,292 - MMP_RS00545 0.15 +0.2 107,529 + MMP_RS00545 0.59 -0.9 107,559 + MMP_RS00545 0.64 -0.4 107,597 + MMP_RS00545 0.71 +0.3 107,974 - -0.9 107,975 + -2.0 107,979 + -1.2 107,998 - +0.1 108,033 + -1.3 108,052 - -0.5 108,062 - -0.9 108,063 + -0.4 108,076 - +0.2 108,076 - +0.1 108,080 - -1.0 108,081 + -0.9
Or see this region's nucleotide sequence