Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00345

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00340 and MMP_RS00345 are separated by 64 nucleotidesMMP_RS00345 and MMP_RS00350 are separated by 108 nucleotidesMMP_RS00350 and MMP_RS00355 are separated by 18 nucleotides MMP_RS00340: MMP_RS00340 - 5 10-methylenetetrahydromethanopterin reductase, at 72,602 to 73,564 _RS00340 MMP_RS00345: MMP_RS00345 - DUF2666 domain-containing protein, at 73,629 to 74,051 _RS00345 MMP_RS00350: MMP_RS00350 - 50S ribosomal protein L18a, at 74,160 to 74,390 _RS00350 MMP_RS00355: MMP_RS00355 - translation initiation factor IF-6, at 74,409 to 75,092 _RS00355 Position (kb) 73 74 75Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 73.405 kb on + strandat 73.533 kb on - strandat 73.617 kb on + strandat 73.690 kb on + strand, within MMP_RS00345at 73.821 kb on + strand, within MMP_RS00345at 73.832 kb on - strand, within MMP_RS00345at 73.857 kb on - strand, within MMP_RS00345at 73.917 kb on - strand, within MMP_RS00345at 74.078 kb on - strandat 74.080 kb on - strandat 74.109 kb on + strandat 74.110 kb on - strandat 74.111 kb on + strandat 74.113 kb on + strandat 74.688 kb on - strandat 74.763 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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73,405 + -3.3
73,533 - -2.0
73,617 + -0.3
73,690 + MMP_RS00345 0.14 +0.9
73,821 + MMP_RS00345 0.45 +0.5
73,832 - MMP_RS00345 0.48 +0.7
73,857 - MMP_RS00345 0.54 +0.6
73,917 - MMP_RS00345 0.68 +0.5
74,078 - +0.2
74,080 - +3.8
74,109 + +1.0
74,110 - +0.8
74,111 + +0.1
74,113 + +0.4
74,688 - -3.9
74,763 - -1.9

Or see this region's nucleotide sequence