Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00285 and MMP_RS00290 are separated by 57 nucleotides MMP_RS00290 and MMP_RS00295 are separated by 38 nucleotides MMP_RS00295 and MMP_RS00300 are separated by 48 nucleotides MMP_RS00300 and MMP_RS00305 are separated by 37 nucleotides MMP_RS00305 and MMP_RS00310 are separated by 1 nucleotides
MMP_RS00285: MMP_RS00285 - TIGR04013 family B12-binding domain/radical SAM domain-containing protein, at 64,389 to 65,498
_RS00285
MMP_RS00290: MMP_RS00290 - hypothetical protein, at 65,556 to 65,828
_RS00290
MMP_RS00295: MMP_RS00295 - gamma carbonic anhydrase family protein, at 65,867 to 66,322
_RS00295
MMP_RS00300: MMP_RS00300 - 6 7-dimethyl-8-ribityllumazine synthase, at 66,371 to 66,784
_RS00300
MMP_RS00305: MMP_RS00305 - phosphoribosyl-ATP diphosphatase, at 66,822 to 67,112
_RS00305
MMP_RS00310: MMP_RS00310 - CBS domain-containing protein, at 67,114 to 68,025
_RS00310
Position (kb) 65
66
67 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 65.009 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.293 kb on + strand, within MMP_RS00285 at 65.371 kb on + strand, within MMP_RS00285 at 65.373 kb on - strand, within MMP_RS00285 at 65.437 kb on + strand at 65.464 kb on - strand at 65.464 kb on - strand at 65.465 kb on + strand at 65.498 kb on - strand at 65.499 kb on + strand at 65.508 kb on - strand at 65.521 kb on - strand at 65.522 kb on + strand at 65.522 kb on + strand at 65.522 kb on + strand at 65.523 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.575 kb on - strand at 65.577 kb on - strand at 65.577 kb on - strand at 65.578 kb on + strand at 65.578 kb on + strand at 65.578 kb on + strand at 65.587 kb on - strand, within MMP_RS00290 at 65.587 kb on - strand, within MMP_RS00290 at 65.619 kb on - strand, within MMP_RS00290 at 65.625 kb on - strand, within MMP_RS00290 at 65.664 kb on - strand, within MMP_RS00290 at 65.755 kb on - strand, within MMP_RS00290 at 65.773 kb on - strand, within MMP_RS00290 at 65.854 kb on - strand at 65.855 kb on + strand at 65.864 kb on + strand at 65.865 kb on - strand at 65.866 kb on + strand at 65.946 kb on - strand, within MMP_RS00295 at 66.252 kb on - strand, within MMP_RS00295 at 66.320 kb on - strand at 66.320 kb on - strand at 66.713 kb on + strand, within MMP_RS00300 at 66.841 kb on - strand at 66.841 kb on - strand at 66.843 kb on - strand at 66.888 kb on - strand, within MMP_RS00305 at 66.889 kb on + strand, within MMP_RS00305 at 66.897 kb on + strand, within MMP_RS00305 at 66.972 kb on + strand, within MMP_RS00305 at 66.975 kb on + strand, within MMP_RS00305 at 67.115 kb on - strand at 67.115 kb on - strand at 67.152 kb on - strand at 67.162 kb on - strand at 67.163 kb on + strand at 67.174 kb on + strand at 67.269 kb on + strand, within MMP_RS00310 at 67.295 kb on - strand, within MMP_RS00310 at 67.297 kb on - strand, within MMP_RS00310
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 65,009 - MMP_RS00285 0.56 +0.0 65,009 - MMP_RS00285 0.56 +0.6 65,293 + MMP_RS00285 0.81 +0.1 65,371 + MMP_RS00285 0.88 +0.5 65,373 - MMP_RS00285 0.89 -0.2 65,437 + +0.5 65,464 - -0.5 65,464 - +0.3 65,465 + +0.5 65,498 - -0.2 65,499 + +0.3 65,508 - -1.8 65,521 - -2.3 65,522 + +0.1 65,522 + -1.3 65,522 + -1.2 65,523 - -2.1 65,575 - -3.4 65,575 - -3.8 65,575 - -2.9 65,575 - -3.1 65,575 - -3.2 65,577 - -1.6 65,577 - -2.7 65,578 + -1.2 65,578 + -1.4 65,578 + -2.1 65,587 - MMP_RS00290 0.11 -2.6 65,587 - MMP_RS00290 0.11 -2.3 65,619 - MMP_RS00290 0.23 -1.5 65,625 - MMP_RS00290 0.25 -2.4 65,664 - MMP_RS00290 0.40 -3.2 65,755 - MMP_RS00290 0.73 -2.9 65,773 - MMP_RS00290 0.79 -2.2 65,854 - -5.1 65,855 + -4.2 65,864 + -4.3 65,865 - -3.9 65,866 + -3.8 65,946 - MMP_RS00295 0.17 -4.8 66,252 - MMP_RS00295 0.84 -3.0 66,320 - -5.0 66,320 - -4.6 66,713 + MMP_RS00300 0.83 -3.3 66,841 - -2.3 66,841 - -4.0 66,843 - -3.3 66,888 - MMP_RS00305 0.23 -1.7 66,889 + MMP_RS00305 0.23 -4.0 66,897 + MMP_RS00305 0.26 -2.9 66,972 + MMP_RS00305 0.52 -2.1 66,975 + MMP_RS00305 0.53 -3.5 67,115 - +0.0 67,115 - -0.3 67,152 - +0.2 67,162 - -0.5 67,163 + +3.9 67,174 + -0.4 67,269 + MMP_RS00310 0.17 -0.1 67,295 - MMP_RS00310 0.20 +0.5 67,297 - MMP_RS00310 0.20 +0.5
Or see this region's nucleotide sequence