Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00275 and MMP_RS00280 are separated by 50 nucleotides MMP_RS00280 and MMP_RS00285 are separated by 4 nucleotides
MMP_RS00275: MMP_RS00275 - polyprenyl synthetase family protein, at 62,928 to 63,881
_RS00275
MMP_RS00280: MMP_RS00280 - DUF2118 domain-containing protein, at 63,932 to 64,384
_RS00280
MMP_RS00285: MMP_RS00285 - TIGR04013 family B12-binding domain/radical SAM domain-containing protein, at 64,389 to 65,498
_RS00285
Position (kb) 63
64
65 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 62.996 kb on - strand at 63.055 kb on + strand at 63.293 kb on - strand at 63.759 kb on - strand at 63.804 kb on - strand at 63.889 kb on - strand at 63.897 kb on - strand at 63.898 kb on + strand at 63.919 kb on - strand at 64.061 kb on - strand, within MMP_RS00280 at 64.071 kb on - strand, within MMP_RS00280 at 64.091 kb on - strand, within MMP_RS00280 at 64.128 kb on - strand, within MMP_RS00280 at 64.147 kb on - strand, within MMP_RS00280 at 64.154 kb on + strand, within MMP_RS00280 at 64.154 kb on + strand, within MMP_RS00280 at 64.162 kb on - strand, within MMP_RS00280 at 64.181 kb on + strand, within MMP_RS00280 at 64.203 kb on + strand, within MMP_RS00280 at 64.287 kb on - strand, within MMP_RS00280 at 64.292 kb on - strand, within MMP_RS00280 at 64.293 kb on + strand, within MMP_RS00280 at 64.293 kb on + strand, within MMP_RS00280 at 64.297 kb on - strand, within MMP_RS00280 at 64.309 kb on + strand, within MMP_RS00280 at 64.323 kb on - strand, within MMP_RS00280 at 64.328 kb on + strand, within MMP_RS00280 at 64.358 kb on - strand at 64.358 kb on - strand at 64.359 kb on + strand at 64.362 kb on - strand at 64.464 kb on + strand at 64.534 kb on - strand, within MMP_RS00285 at 64.535 kb on + strand, within MMP_RS00285 at 64.570 kb on - strand, within MMP_RS00285 at 64.632 kb on + strand, within MMP_RS00285 at 64.722 kb on - strand, within MMP_RS00285 at 64.766 kb on - strand, within MMP_RS00285 at 64.766 kb on - strand, within MMP_RS00285 at 64.777 kb on - strand, within MMP_RS00285 at 64.788 kb on - strand, within MMP_RS00285 at 64.824 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.009 kb on - strand, within MMP_RS00285 at 65.293 kb on + strand, within MMP_RS00285 at 65.371 kb on + strand, within MMP_RS00285 at 65.373 kb on - strand, within MMP_RS00285
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 62,996 - -2.3 63,055 + -2.7 63,293 - -3.5 63,759 - -3.3 63,804 - -4.0 63,889 - +0.7 63,897 - -0.3 63,898 + -0.0 63,919 - +0.3 64,061 - MMP_RS00280 0.28 +0.2 64,071 - MMP_RS00280 0.31 +0.1 64,091 - MMP_RS00280 0.35 +0.8 64,128 - MMP_RS00280 0.43 +0.5 64,147 - MMP_RS00280 0.47 -0.5 64,154 + MMP_RS00280 0.49 +0.3 64,154 + MMP_RS00280 0.49 +3.7 64,162 - MMP_RS00280 0.51 +0.5 64,181 + MMP_RS00280 0.55 +0.2 64,203 + MMP_RS00280 0.60 +0.4 64,287 - MMP_RS00280 0.78 -0.0 64,292 - MMP_RS00280 0.79 +0.3 64,293 + MMP_RS00280 0.80 -0.1 64,293 + MMP_RS00280 0.80 +0.4 64,297 - MMP_RS00280 0.81 +0.6 64,309 + MMP_RS00280 0.83 +0.2 64,323 - MMP_RS00280 0.86 +0.1 64,328 + MMP_RS00280 0.87 -0.1 64,358 - +0.1 64,358 - +0.5 64,359 + +0.2 64,362 - -0.2 64,464 + +0.4 64,534 - MMP_RS00285 0.13 +0.3 64,535 + MMP_RS00285 0.13 -0.2 64,570 - MMP_RS00285 0.16 +0.2 64,632 + MMP_RS00285 0.22 +0.1 64,722 - MMP_RS00285 0.30 -0.2 64,766 - MMP_RS00285 0.34 -0.1 64,766 - MMP_RS00285 0.34 +0.5 64,777 - MMP_RS00285 0.35 +0.1 64,788 - MMP_RS00285 0.36 -0.9 64,824 - MMP_RS00285 0.39 -0.1 65,009 - MMP_RS00285 0.56 +0.0 65,009 - MMP_RS00285 0.56 +0.6 65,293 + MMP_RS00285 0.81 +0.1 65,371 + MMP_RS00285 0.88 +0.5 65,373 - MMP_RS00285 0.89 -0.2
Or see this region's nucleotide sequence