Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00075 and MMP_RS00080 are separated by 26 nucleotides MMP_RS00080 and MMP_RS00085 are separated by 42 nucleotides
MMP_RS00075: MMP_RS00075 - hypothetical protein, at 19,370 to 20,479
_RS00075
MMP_RS00080: MMP_RS00080 - RecB-family nuclease, at 20,506 to 20,931
_RS00080
MMP_RS00085: MMP_RS00085 - DNA cytosine methyltransferase, at 20,974 to 22,041
_RS00085
Position (kb)
20
21 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 19.527 kb on + strand at 19.562 kb on - strand at 19.563 kb on + strand at 19.563 kb on + strand at 19.859 kb on + strand at 20.020 kb on + strand at 20.084 kb on - strand at 20.135 kb on - strand at 20.705 kb on + strand, within MMP_RS00080 at 20.707 kb on - strand, within MMP_RS00080 at 20.731 kb on - strand, within MMP_RS00080 at 20.839 kb on + strand, within MMP_RS00080 at 20.842 kb on - strand, within MMP_RS00080 at 20.961 kb on + strand at 20.975 kb on - strand at 20.976 kb on + strand at 20.979 kb on - strand at 20.979 kb on - strand at 20.980 kb on + strand at 21.054 kb on + strand at 21.128 kb on + strand, within MMP_RS00085 at 21.209 kb on - strand, within MMP_RS00085 at 21.271 kb on + strand, within MMP_RS00085 at 21.518 kb on - strand, within MMP_RS00085 at 21.523 kb on - strand, within MMP_RS00085 at 21.567 kb on - strand, within MMP_RS00085 at 21.588 kb on + strand, within MMP_RS00085 at 21.692 kb on + strand, within MMP_RS00085 at 21.713 kb on - strand, within MMP_RS00085 at 21.721 kb on + strand, within MMP_RS00085 at 21.743 kb on - strand, within MMP_RS00085 at 21.763 kb on - strand, within MMP_RS00085 at 21.779 kb on + strand, within MMP_RS00085 at 21.826 kb on - strand, within MMP_RS00085 at 21.826 kb on - strand, within MMP_RS00085 at 21.827 kb on + strand, within MMP_RS00085 at 21.827 kb on + strand, within MMP_RS00085
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 19,527 + -2.1 19,562 - -2.6 19,563 + -3.1 19,563 + -3.0 19,859 + -3.2 20,020 + -1.9 20,084 - +0.1 20,135 - -3.3 20,705 + MMP_RS00080 0.47 +0.4 20,707 - MMP_RS00080 0.47 +0.4 20,731 - MMP_RS00080 0.53 +0.6 20,839 + MMP_RS00080 0.78 +0.7 20,842 - MMP_RS00080 0.79 +0.8 20,961 + +0.6 20,975 - +0.1 20,976 + +0.1 20,979 - -0.0 20,979 - +0.6 20,980 + +0.3 21,054 + +0.6 21,128 + MMP_RS00085 0.14 +0.3 21,209 - MMP_RS00085 0.22 +0.1 21,271 + MMP_RS00085 0.28 -1.6 21,518 - MMP_RS00085 0.51 +0.2 21,523 - MMP_RS00085 0.51 +0.3 21,567 - MMP_RS00085 0.56 +0.3 21,588 + MMP_RS00085 0.57 +1.0 21,692 + MMP_RS00085 0.67 +0.2 21,713 - MMP_RS00085 0.69 +0.4 21,721 + MMP_RS00085 0.70 -0.1 21,743 - MMP_RS00085 0.72 +1.1 21,763 - MMP_RS00085 0.74 +0.7 21,779 + MMP_RS00085 0.75 +0.2 21,826 - MMP_RS00085 0.80 -0.1 21,826 - MMP_RS00085 0.80 +0.0 21,827 + MMP_RS00085 0.80 +0.4 21,827 + MMP_RS00085 0.80 +0.5
Or see this region's nucleotide sequence