Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25325 and CA265_RS25330 overlap by 10 nucleotides CA265_RS25330 and CA265_RS25335 are separated by 212 nucleotides CA265_RS25335 and CA265_RS25340 are separated by 142 nucleotides
CA265_RS25325: CA265_RS25325 - ATPase, at 6,157,350 to 6,158,525
_RS25325
CA265_RS25330: CA265_RS25330 - DoxX family protein, at 6,158,516 to 6,158,974
_RS25330
CA265_RS25335: CA265_RS25335 - hypothetical protein, at 6,159,187 to 6,159,297
_RS25335
CA265_RS25340: CA265_RS25340 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 6,159,440 to 6,160,852
_RS25340
Position (kb)
6158
6159 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6157.526 kb on + strand, within CA265_RS25325 at 6157.527 kb on - strand, within CA265_RS25325 at 6157.527 kb on - strand, within CA265_RS25325 at 6157.527 kb on - strand, within CA265_RS25325 at 6157.529 kb on - strand, within CA265_RS25325 at 6157.529 kb on - strand, within CA265_RS25325 at 6157.529 kb on - strand, within CA265_RS25325 at 6157.558 kb on + strand, within CA265_RS25325 at 6157.559 kb on - strand, within CA265_RS25325 at 6157.634 kb on + strand, within CA265_RS25325 at 6157.635 kb on - strand, within CA265_RS25325 at 6157.646 kb on + strand, within CA265_RS25325 at 6157.647 kb on - strand, within CA265_RS25325 at 6157.714 kb on + strand, within CA265_RS25325 at 6157.732 kb on - strand, within CA265_RS25325 at 6157.781 kb on - strand, within CA265_RS25325 at 6157.784 kb on + strand, within CA265_RS25325 at 6157.829 kb on - strand, within CA265_RS25325 at 6157.884 kb on + strand, within CA265_RS25325 at 6157.884 kb on + strand, within CA265_RS25325 at 6157.898 kb on + strand, within CA265_RS25325 at 6157.974 kb on - strand, within CA265_RS25325 at 6157.974 kb on - strand, within CA265_RS25325 at 6158.006 kb on + strand, within CA265_RS25325 at 6158.006 kb on + strand, within CA265_RS25325 at 6158.015 kb on + strand, within CA265_RS25325 at 6158.038 kb on - strand, within CA265_RS25325 at 6158.059 kb on + strand, within CA265_RS25325 at 6158.085 kb on + strand, within CA265_RS25325 at 6158.093 kb on + strand, within CA265_RS25325 at 6158.128 kb on + strand, within CA265_RS25325 at 6158.135 kb on - strand, within CA265_RS25325 at 6158.170 kb on + strand, within CA265_RS25325 at 6158.203 kb on + strand, within CA265_RS25325 at 6158.206 kb on + strand, within CA265_RS25325 at 6158.275 kb on - strand, within CA265_RS25325 at 6158.280 kb on + strand, within CA265_RS25325 at 6158.281 kb on - strand, within CA265_RS25325 at 6158.348 kb on - strand, within CA265_RS25325 at 6158.421 kb on - strand at 6158.424 kb on - strand at 6158.444 kb on + strand at 6158.456 kb on + strand at 6158.510 kb on + strand at 6158.511 kb on - strand at 6158.512 kb on + strand at 6158.579 kb on + strand, within CA265_RS25330 at 6158.775 kb on - strand, within CA265_RS25330 at 6158.816 kb on + strand, within CA265_RS25330 at 6158.901 kb on - strand, within CA265_RS25330 at 6159.007 kb on + strand at 6159.055 kb on + strand at 6159.170 kb on + strand at 6159.170 kb on + strand at 6159.170 kb on + strand at 6159.170 kb on + strand at 6159.171 kb on - strand at 6159.200 kb on + strand, within CA265_RS25335 at 6159.445 kb on + strand at 6159.460 kb on + strand at 6159.470 kb on - strand at 6159.483 kb on + strand at 6159.484 kb on - strand at 6159.577 kb on - strand at 6159.697 kb on + strand, within CA265_RS25340 at 6159.716 kb on + strand, within CA265_RS25340 at 6159.717 kb on - strand, within CA265_RS25340 at 6159.737 kb on - strand, within CA265_RS25340 at 6159.780 kb on + strand, within CA265_RS25340 at 6159.780 kb on + strand, within CA265_RS25340 at 6159.802 kb on + strand, within CA265_RS25340 at 6159.807 kb on + strand, within CA265_RS25340 at 6159.839 kb on - strand, within CA265_RS25340 at 6159.888 kb on + strand, within CA265_RS25340 at 6159.962 kb on - strand, within CA265_RS25340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 6,157,526 + CA265_RS25325 0.15 +0.1 6,157,527 - CA265_RS25325 0.15 +1.9 6,157,527 - CA265_RS25325 0.15 +0.2 6,157,527 - CA265_RS25325 0.15 +0.2 6,157,529 - CA265_RS25325 0.15 +0.0 6,157,529 - CA265_RS25325 0.15 -0.7 6,157,529 - CA265_RS25325 0.15 +0.3 6,157,558 + CA265_RS25325 0.18 -0.6 6,157,559 - CA265_RS25325 0.18 -0.2 6,157,634 + CA265_RS25325 0.24 -0.7 6,157,635 - CA265_RS25325 0.24 -1.6 6,157,646 + CA265_RS25325 0.25 +0.5 6,157,647 - CA265_RS25325 0.25 +0.4 6,157,714 + CA265_RS25325 0.31 -0.6 6,157,732 - CA265_RS25325 0.32 +0.6 6,157,781 - CA265_RS25325 0.37 -1.0 6,157,784 + CA265_RS25325 0.37 -0.5 6,157,829 - CA265_RS25325 0.41 -0.3 6,157,884 + CA265_RS25325 0.45 +0.4 6,157,884 + CA265_RS25325 0.45 +0.2 6,157,898 + CA265_RS25325 0.47 +0.4 6,157,974 - CA265_RS25325 0.53 +0.0 6,157,974 - CA265_RS25325 0.53 +0.7 6,158,006 + CA265_RS25325 0.56 -1.0 6,158,006 + CA265_RS25325 0.56 -0.6 6,158,015 + CA265_RS25325 0.57 +0.3 6,158,038 - CA265_RS25325 0.59 +1.4 6,158,059 + CA265_RS25325 0.60 +0.2 6,158,085 + CA265_RS25325 0.62 -0.7 6,158,093 + CA265_RS25325 0.63 -0.5 6,158,128 + CA265_RS25325 0.66 -1.3 6,158,135 - CA265_RS25325 0.67 -0.7 6,158,170 + CA265_RS25325 0.70 +0.3 6,158,203 + CA265_RS25325 0.73 +1.1 6,158,206 + CA265_RS25325 0.73 +0.6 6,158,275 - CA265_RS25325 0.79 +0.5 6,158,280 + CA265_RS25325 0.79 +0.8 6,158,281 - CA265_RS25325 0.79 +0.5 6,158,348 - CA265_RS25325 0.85 -0.4 6,158,421 - -0.1 6,158,424 - -0.4 6,158,444 + +0.5 6,158,456 + -0.1 6,158,510 + -0.3 6,158,511 - -0.3 6,158,512 + +0.6 6,158,579 + CA265_RS25330 0.14 -0.6 6,158,775 - CA265_RS25330 0.56 -2.0 6,158,816 + CA265_RS25330 0.65 -1.2 6,158,901 - CA265_RS25330 0.84 -0.5 6,159,007 + -0.3 6,159,055 + -0.9 6,159,170 + +0.9 6,159,170 + +0.0 6,159,170 + -0.4 6,159,170 + +0.2 6,159,171 - -0.1 6,159,200 + CA265_RS25335 0.12 -0.2 6,159,445 + +1.0 6,159,460 + -1.0 6,159,470 - -0.2 6,159,483 + +0.2 6,159,484 - -0.4 6,159,577 - +0.1 6,159,697 + CA265_RS25340 0.18 +0.2 6,159,716 + CA265_RS25340 0.20 +0.6 6,159,717 - CA265_RS25340 0.20 +0.6 6,159,737 - CA265_RS25340 0.21 +1.3 6,159,780 + CA265_RS25340 0.24 +0.1 6,159,780 + CA265_RS25340 0.24 -0.1 6,159,802 + CA265_RS25340 0.26 -0.0 6,159,807 + CA265_RS25340 0.26 +0.6 6,159,839 - CA265_RS25340 0.28 -0.0 6,159,888 + CA265_RS25340 0.32 +0.1 6,159,962 - CA265_RS25340 0.37 +0.3
Or see this region's nucleotide sequence