Strain Fitness in Pedobacter sp. GW460-11-11-14-LB5 around CA265_RS25270

Experiment: R2A with Neomycin 0.004 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Neomycin 0.004 mg/ml
6,146,112	+		CA265_RS25255	0.74	+0.8
6,146,113	-		CA265_RS25255	0.74	-0.1
6,146,113	-		CA265_RS25255	0.74	-0.6
6,146,113	-		CA265_RS25255	0.74	+0.5
6,146,115	-		CA265_RS25255	0.74	+0.0
6,146,115	-		CA265_RS25255	0.74	+0.3
6,146,131	-		CA265_RS25255	0.78	+0.0
6,146,133	-		CA265_RS25255	0.79	+0.8
6,146,133	-		CA265_RS25255	0.79	+0.6
6,146,133	-		CA265_RS25255	0.79	+0.2
6,146,141	-		CA265_RS25255	0.80	+0.2
6,146,167	+		CA265_RS25255	0.86	+0.1
6,146,168	-		CA265_RS25255	0.87	+1.0
6,146,203	+				-0.3
6,146,321	+		CA265_RS25260	0.36	+0.2
6,146,341	-		CA265_RS25260	0.42	+1.7
6,146,362	+		CA265_RS25260	0.49	-0.8
6,146,363	-		CA265_RS25260	0.49	-0.2
6,146,407	-		CA265_RS25260	0.63	-0.5
6,146,491	+		CA265_RS25260	0.89	+0.2
6,146,505	-				+0.5
6,146,506	+				+1.3
6,146,506	+				+0.0
6,146,528	+				-0.3
6,146,528	+				+0.5
6,146,530	+				-0.1
6,146,530	+				-0.2
6,146,535	-				+0.0
6,146,567	+				+0.2
6,146,624	-				+0.4
6,146,624	-				-0.6
6,146,624	-				+0.1
6,146,627	+				+0.8
6,146,786	-		CA265_RS25265	0.18	-0.1
6,146,797	+		CA265_RS25265	0.22	+0.3
6,146,801	-		CA265_RS25265	0.23	-0.6
6,146,809	+		CA265_RS25265	0.26	-0.7
6,146,810	-		CA265_RS25265	0.27	-0.8
6,146,812	+		CA265_RS25265	0.27	-0.5
6,146,859	-		CA265_RS25265	0.44	-2.1
6,146,950	+		CA265_RS25265	0.77	+0.4
6,146,951	-		CA265_RS25265	0.77	-0.4
6,147,019	+				+0.2
6,147,109	+				-0.4
6,147,109	+				+0.1
6,147,114	+				-1.9
6,147,206	+		CA265_RS25270	0.23	-1.8
6,147,372	+		CA265_RS25270	0.64	-0.1
6,147,375	-		CA265_RS25270	0.64	-1.4
6,147,548	+				-0.4
6,147,567	+				+0.1
6,147,567	+				+0.2
6,147,568	-				-0.1
6,147,626	+				-0.1
6,147,627	-				+0.8
6,147,666	+				-0.0
6,147,667	-				+0.2
6,147,714	+				+0.5
6,147,787	+				-0.1
6,147,797	-				+0.9
6,147,896	-				-0.1
6,147,973	+		CA265_RS25275	0.20	+0.5
6,148,095	+		CA265_RS25275	0.35	-0.1
6,148,096	-		CA265_RS25275	0.35	+0.1
6,148,174	-		CA265_RS25275	0.45	+0.3
6,148,230	+		CA265_RS25275	0.52	+0.4
6,148,282	+				+0.0
6,148,283	-		CA265_RS25275	0.59	+0.0
6,148,336	+		CA265_RS25275	0.66	-1.7
6,148,369	+		CA265_RS25275	0.70	-0.6
6,148,370	-		CA265_RS25275	0.70	-0.1
6,148,414	-		CA265_RS25275	0.75	-0.6
6,148,520	+		CA265_RS25275	0.89	-0.1
6,148,520	+		CA265_RS25275	0.89	-0.4