Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23985 and CA265_RS23990 are separated by 30 nucleotides CA265_RS23990 and CA265_RS23995 are separated by 48 nucleotides
CA265_RS23985: CA265_RS23985 - gliding motility lipoprotein GldB, at 5,846,865 to 5,847,899
_RS23985
CA265_RS23990: CA265_RS23990 - hypothetical protein, at 5,847,930 to 5,848,613
_RS23990
CA265_RS23995: CA265_RS23995 - NAD+ synthase, at 5,848,662 to 5,850,302
_RS23995
Position (kb)
5847
5848
5849 Strain fitness (log2 ratio)
-1
0
1 at 5846.967 kb on - strand at 5847.171 kb on - strand, within CA265_RS23985 at 5847.258 kb on + strand, within CA265_RS23985 at 5847.270 kb on - strand, within CA265_RS23985 at 5847.308 kb on - strand, within CA265_RS23985 at 5847.319 kb on - strand, within CA265_RS23985 at 5847.332 kb on + strand, within CA265_RS23985 at 5847.334 kb on + strand, within CA265_RS23985 at 5847.334 kb on + strand, within CA265_RS23985 at 5847.335 kb on - strand, within CA265_RS23985 at 5847.335 kb on - strand, within CA265_RS23985 at 5847.362 kb on - strand, within CA265_RS23985 at 5847.362 kb on - strand, within CA265_RS23985 at 5847.364 kb on - strand, within CA265_RS23985 at 5847.364 kb on - strand, within CA265_RS23985 at 5847.383 kb on + strand, within CA265_RS23985 at 5847.521 kb on - strand, within CA265_RS23985 at 5847.521 kb on - strand, within CA265_RS23985 at 5847.567 kb on + strand, within CA265_RS23985 at 5847.567 kb on + strand, within CA265_RS23985 at 5847.568 kb on - strand, within CA265_RS23985 at 5847.588 kb on + strand, within CA265_RS23985 at 5847.611 kb on - strand, within CA265_RS23985 at 5847.628 kb on - strand, within CA265_RS23985 at 5847.655 kb on + strand, within CA265_RS23985 at 5847.655 kb on + strand, within CA265_RS23985 at 5847.681 kb on - strand, within CA265_RS23985 at 5847.726 kb on + strand, within CA265_RS23985 at 5847.736 kb on - strand, within CA265_RS23985 at 5847.793 kb on - strand, within CA265_RS23985 at 5847.793 kb on - strand, within CA265_RS23985 at 5847.800 kb on + strand at 5847.803 kb on + strand at 5847.803 kb on + strand at 5847.803 kb on + strand at 5847.868 kb on + strand at 5847.892 kb on + strand at 5847.892 kb on + strand at 5847.892 kb on + strand at 5847.893 kb on - strand at 5847.913 kb on - strand at 5847.917 kb on - strand at 5847.925 kb on - strand at 5847.926 kb on + strand at 5847.927 kb on - strand at 5847.952 kb on + strand at 5848.016 kb on + strand, within CA265_RS23990 at 5848.021 kb on + strand, within CA265_RS23990 at 5848.085 kb on - strand, within CA265_RS23990 at 5848.172 kb on - strand, within CA265_RS23990 at 5848.192 kb on + strand, within CA265_RS23990 at 5848.192 kb on + strand, within CA265_RS23990 at 5848.193 kb on - strand, within CA265_RS23990 at 5848.218 kb on - strand, within CA265_RS23990 at 5848.411 kb on + strand, within CA265_RS23990 at 5848.427 kb on + strand, within CA265_RS23990 at 5848.434 kb on + strand, within CA265_RS23990 at 5848.652 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,846,967 - -0.4 5,847,171 - CA265_RS23985 0.30 +0.9 5,847,258 + CA265_RS23985 0.38 +0.5 5,847,270 - CA265_RS23985 0.39 +0.6 5,847,308 - CA265_RS23985 0.43 +1.4 5,847,319 - CA265_RS23985 0.44 +0.1 5,847,332 + CA265_RS23985 0.45 +0.3 5,847,334 + CA265_RS23985 0.45 +0.7 5,847,334 + CA265_RS23985 0.45 +0.9 5,847,335 - CA265_RS23985 0.45 +1.0 5,847,335 - CA265_RS23985 0.45 +0.7 5,847,362 - CA265_RS23985 0.48 +0.5 5,847,362 - CA265_RS23985 0.48 -0.0 5,847,364 - CA265_RS23985 0.48 -0.1 5,847,364 - CA265_RS23985 0.48 -0.3 5,847,383 + CA265_RS23985 0.50 +0.1 5,847,521 - CA265_RS23985 0.63 -1.4 5,847,521 - CA265_RS23985 0.63 +0.8 5,847,567 + CA265_RS23985 0.68 -0.4 5,847,567 + CA265_RS23985 0.68 +0.3 5,847,568 - CA265_RS23985 0.68 -0.5 5,847,588 + CA265_RS23985 0.70 +1.2 5,847,611 - CA265_RS23985 0.72 -0.1 5,847,628 - CA265_RS23985 0.74 +0.2 5,847,655 + CA265_RS23985 0.76 -0.3 5,847,655 + CA265_RS23985 0.76 -1.3 5,847,681 - CA265_RS23985 0.79 +0.1 5,847,726 + CA265_RS23985 0.83 +0.7 5,847,736 - CA265_RS23985 0.84 +0.1 5,847,793 - CA265_RS23985 0.90 +1.1 5,847,793 - CA265_RS23985 0.90 +0.3 5,847,800 + +0.6 5,847,803 + +0.4 5,847,803 + +0.3 5,847,803 + +1.1 5,847,868 + +1.2 5,847,892 + +1.6 5,847,892 + +0.1 5,847,892 + +0.3 5,847,893 - +1.0 5,847,913 - -0.4 5,847,917 - -0.1 5,847,925 - +0.2 5,847,926 + -0.1 5,847,927 - -0.7 5,847,952 + -1.3 5,848,016 + CA265_RS23990 0.13 -0.5 5,848,021 + CA265_RS23990 0.13 -0.8 5,848,085 - CA265_RS23990 0.23 -0.6 5,848,172 - CA265_RS23990 0.35 +0.1 5,848,192 + CA265_RS23990 0.38 -0.8 5,848,192 + CA265_RS23990 0.38 +0.1 5,848,193 - CA265_RS23990 0.38 -1.6 5,848,218 - CA265_RS23990 0.42 +0.1 5,848,411 + CA265_RS23990 0.70 -0.2 5,848,427 + CA265_RS23990 0.73 -1.7 5,848,434 + CA265_RS23990 0.74 -1.2 5,848,652 + +0.6
Or see this region's nucleotide sequence