Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23700 and CA265_RS23705 are separated by 189 nucleotides CA265_RS23705 and CA265_RS23710 are separated by 183 nucleotides CA265_RS23710 and CA265_RS23715 are separated by 3 nucleotides
CA265_RS23700: CA265_RS23700 - hypothetical protein, at 5,787,541 to 5,788,635
_RS23700
CA265_RS23705: CA265_RS23705 - esterase, at 5,788,825 to 5,789,532
_RS23705
CA265_RS23710: CA265_RS23710 - esterase, at 5,789,716 to 5,790,528
_RS23710
CA265_RS23715: CA265_RS23715 - hypothetical protein, at 5,790,532 to 5,791,440
_RS23715
Position (kb)
5788
5789
5790 Strain fitness (log2 ratio)
-1
0
1 at 5787.829 kb on - strand, within CA265_RS23700 at 5787.927 kb on + strand, within CA265_RS23700 at 5787.927 kb on + strand, within CA265_RS23700 at 5787.985 kb on - strand, within CA265_RS23700 at 5788.046 kb on + strand, within CA265_RS23700 at 5788.046 kb on + strand, within CA265_RS23700 at 5788.167 kb on + strand, within CA265_RS23700 at 5788.168 kb on - strand, within CA265_RS23700 at 5788.206 kb on + strand, within CA265_RS23700 at 5788.209 kb on - strand, within CA265_RS23700 at 5788.226 kb on + strand, within CA265_RS23700 at 5788.337 kb on - strand, within CA265_RS23700 at 5788.459 kb on + strand, within CA265_RS23700 at 5788.498 kb on + strand, within CA265_RS23700 at 5788.516 kb on + strand, within CA265_RS23700 at 5788.615 kb on + strand at 5788.823 kb on - strand at 5788.826 kb on + strand at 5788.965 kb on + strand, within CA265_RS23705 at 5789.070 kb on - strand, within CA265_RS23705 at 5789.121 kb on + strand, within CA265_RS23705 at 5789.157 kb on + strand, within CA265_RS23705 at 5789.252 kb on + strand, within CA265_RS23705 at 5789.277 kb on + strand, within CA265_RS23705 at 5789.310 kb on - strand, within CA265_RS23705 at 5789.317 kb on + strand, within CA265_RS23705 at 5789.317 kb on + strand, within CA265_RS23705 at 5789.367 kb on + strand, within CA265_RS23705 at 5789.368 kb on - strand, within CA265_RS23705 at 5789.451 kb on + strand, within CA265_RS23705 at 5789.452 kb on - strand, within CA265_RS23705 at 5789.514 kb on + strand at 5789.557 kb on - strand at 5789.587 kb on - strand at 5789.644 kb on - strand at 5789.723 kb on - strand at 5789.783 kb on + strand at 5789.801 kb on + strand, within CA265_RS23710 at 5789.805 kb on + strand, within CA265_RS23710 at 5789.805 kb on + strand, within CA265_RS23710 at 5789.806 kb on - strand, within CA265_RS23710 at 5789.809 kb on + strand, within CA265_RS23710 at 5789.810 kb on - strand, within CA265_RS23710 at 5789.810 kb on - strand, within CA265_RS23710 at 5789.810 kb on - strand, within CA265_RS23710 at 5789.858 kb on + strand, within CA265_RS23710 at 5789.903 kb on + strand, within CA265_RS23710 at 5789.904 kb on - strand, within CA265_RS23710 at 5789.940 kb on - strand, within CA265_RS23710 at 5789.983 kb on + strand, within CA265_RS23710 at 5789.984 kb on - strand, within CA265_RS23710 at 5789.984 kb on - strand, within CA265_RS23710 at 5790.036 kb on - strand, within CA265_RS23710 at 5790.038 kb on - strand, within CA265_RS23710 at 5790.065 kb on + strand, within CA265_RS23710 at 5790.076 kb on - strand, within CA265_RS23710 at 5790.141 kb on - strand, within CA265_RS23710 at 5790.261 kb on + strand, within CA265_RS23710 at 5790.262 kb on - strand, within CA265_RS23710 at 5790.283 kb on - strand, within CA265_RS23710 at 5790.358 kb on - strand, within CA265_RS23710 at 5790.392 kb on + strand, within CA265_RS23710 at 5790.420 kb on + strand, within CA265_RS23710 at 5790.442 kb on + strand, within CA265_RS23710 at 5790.442 kb on + strand, within CA265_RS23710
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,787,829 - CA265_RS23700 0.26 +1.4 5,787,927 + CA265_RS23700 0.35 -0.9 5,787,927 + CA265_RS23700 0.35 +0.8 5,787,985 - CA265_RS23700 0.41 -0.5 5,788,046 + CA265_RS23700 0.46 -0.5 5,788,046 + CA265_RS23700 0.46 -0.5 5,788,167 + CA265_RS23700 0.57 +0.8 5,788,168 - CA265_RS23700 0.57 +1.2 5,788,206 + CA265_RS23700 0.61 -0.2 5,788,209 - CA265_RS23700 0.61 -0.1 5,788,226 + CA265_RS23700 0.63 -0.4 5,788,337 - CA265_RS23700 0.73 +0.8 5,788,459 + CA265_RS23700 0.84 -0.1 5,788,498 + CA265_RS23700 0.87 -0.1 5,788,516 + CA265_RS23700 0.89 +0.3 5,788,615 + -0.2 5,788,823 - -0.2 5,788,826 + +0.2 5,788,965 + CA265_RS23705 0.20 +0.6 5,789,070 - CA265_RS23705 0.35 +0.9 5,789,121 + CA265_RS23705 0.42 +0.2 5,789,157 + CA265_RS23705 0.47 +1.3 5,789,252 + CA265_RS23705 0.60 -0.2 5,789,277 + CA265_RS23705 0.64 +0.4 5,789,310 - CA265_RS23705 0.69 -1.1 5,789,317 + CA265_RS23705 0.69 -0.5 5,789,317 + CA265_RS23705 0.69 +1.2 5,789,367 + CA265_RS23705 0.77 +1.0 5,789,368 - CA265_RS23705 0.77 -1.4 5,789,451 + CA265_RS23705 0.88 +1.2 5,789,452 - CA265_RS23705 0.89 +1.2 5,789,514 + +0.4 5,789,557 - -1.7 5,789,587 - -0.8 5,789,644 - -0.9 5,789,723 - -1.1 5,789,783 + -0.3 5,789,801 + CA265_RS23710 0.10 +0.6 5,789,805 + CA265_RS23710 0.11 -1.1 5,789,805 + CA265_RS23710 0.11 -0.2 5,789,806 - CA265_RS23710 0.11 -0.7 5,789,809 + CA265_RS23710 0.11 -0.1 5,789,810 - CA265_RS23710 0.12 -0.8 5,789,810 - CA265_RS23710 0.12 -1.1 5,789,810 - CA265_RS23710 0.12 -0.1 5,789,858 + CA265_RS23710 0.17 +0.0 5,789,903 + CA265_RS23710 0.23 +0.1 5,789,904 - CA265_RS23710 0.23 -1.2 5,789,940 - CA265_RS23710 0.28 -0.4 5,789,983 + CA265_RS23710 0.33 +0.8 5,789,984 - CA265_RS23710 0.33 -0.5 5,789,984 - CA265_RS23710 0.33 -0.7 5,790,036 - CA265_RS23710 0.39 -1.0 5,790,038 - CA265_RS23710 0.40 -0.8 5,790,065 + CA265_RS23710 0.43 +0.1 5,790,076 - CA265_RS23710 0.44 -1.2 5,790,141 - CA265_RS23710 0.52 +0.2 5,790,261 + CA265_RS23710 0.67 -0.3 5,790,262 - CA265_RS23710 0.67 +0.7 5,790,283 - CA265_RS23710 0.70 +0.2 5,790,358 - CA265_RS23710 0.79 +0.6 5,790,392 + CA265_RS23710 0.83 +1.7 5,790,420 + CA265_RS23710 0.87 +0.8 5,790,442 + CA265_RS23710 0.89 +0.1 5,790,442 + CA265_RS23710 0.89 +0.6
Or see this region's nucleotide sequence