Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23555 and CA265_RS23560 are separated by 60 nucleotides CA265_RS23560 and CA265_RS23565 are separated by 72 nucleotides CA265_RS23565 and CA265_RS23570 overlap by 1 nucleotides
CA265_RS23555: CA265_RS23555 - NADH-quinone oxidoreductase subunit F, at 5,756,954 to 5,758,321
_RS23555
CA265_RS23560: CA265_RS23560 - DNA-binding protein, at 5,758,382 to 5,758,921
_RS23560
CA265_RS23565: CA265_RS23565 - NADH dehydrogenase, at 5,758,994 to 5,760,004
_RS23565
CA265_RS23570: CA265_RS23570 - NADH-quinone oxidoreductase subunit H, at 5,760,004 to 5,761,041
_RS23570
Position (kb) 5758
5759
5760
5761 Strain fitness (log2 ratio) -2
-1
0
1 at 5758.262 kb on + strand at 5758.375 kb on + strand at 5758.425 kb on + strand at 5758.437 kb on + strand, within CA265_RS23560 at 5758.467 kb on + strand, within CA265_RS23560 at 5758.467 kb on + strand, within CA265_RS23560 at 5758.504 kb on + strand, within CA265_RS23560 at 5758.534 kb on + strand, within CA265_RS23560 at 5758.614 kb on + strand, within CA265_RS23560 at 5758.614 kb on + strand, within CA265_RS23560 at 5758.707 kb on + strand, within CA265_RS23560 at 5758.747 kb on + strand, within CA265_RS23560 at 5758.762 kb on - strand at 5758.762 kb on - strand, within CA265_RS23560 at 5758.813 kb on + strand, within CA265_RS23560 at 5758.814 kb on - strand, within CA265_RS23560 at 5758.860 kb on + strand, within CA265_RS23560 at 5758.860 kb on + strand, within CA265_RS23560 at 5758.959 kb on + strand at 5758.974 kb on + strand at 5758.983 kb on + strand at 5759.821 kb on + strand, within CA265_RS23565 at 5759.887 kb on + strand, within CA265_RS23565 at 5759.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,758,262 + +0.2 5,758,375 + -1.0 5,758,425 + -1.6 5,758,437 + CA265_RS23560 0.10 -2.1 5,758,467 + CA265_RS23560 0.16 -0.6 5,758,467 + CA265_RS23560 0.16 -1.1 5,758,504 + CA265_RS23560 0.23 -0.9 5,758,534 + CA265_RS23560 0.28 -1.1 5,758,614 + CA265_RS23560 0.43 -0.5 5,758,614 + CA265_RS23560 0.43 -1.5 5,758,707 + CA265_RS23560 0.60 -2.0 5,758,747 + CA265_RS23560 0.68 -1.1 5,758,762 - +1.7 5,758,762 - CA265_RS23560 0.70 -1.6 5,758,813 + CA265_RS23560 0.80 -1.3 5,758,814 - CA265_RS23560 0.80 -0.3 5,758,860 + CA265_RS23560 0.89 -1.0 5,758,860 + CA265_RS23560 0.89 +0.1 5,758,959 + -0.3 5,758,974 + -1.2 5,758,983 + -0.5 5,759,821 + CA265_RS23565 0.82 +0.1 5,759,887 + CA265_RS23565 0.88 -1.7 5,759,982 + -0.5
Or see this region's nucleotide sequence