Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23185 and CA265_RS23190 are separated by 359 nucleotides CA265_RS23190 and CA265_RS23195 are separated by 88 nucleotides CA265_RS23195 and CA265_RS23200 are separated by 57 nucleotides
CA265_RS23185: CA265_RS23185 - aminopeptidase, at 5,671,821 to 5,673,701
_RS23185
CA265_RS23190: CA265_RS23190 - transcription elongation factor GreA, at 5,674,061 to 5,674,534
_RS23190
CA265_RS23195: CA265_RS23195 - HIT family protein, at 5,674,623 to 5,675,021
_RS23195
CA265_RS23200: CA265_RS23200 - hypothetical protein, at 5,675,079 to 5,675,939
_RS23200
Position (kb)
5674
5675 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5673.127 kb on + strand, within CA265_RS23185 at 5673.128 kb on - strand, within CA265_RS23185 at 5673.158 kb on + strand, within CA265_RS23185 at 5673.158 kb on + strand, within CA265_RS23185 at 5673.159 kb on - strand, within CA265_RS23185 at 5673.159 kb on - strand, within CA265_RS23185 at 5673.159 kb on - strand, within CA265_RS23185 at 5673.161 kb on - strand, within CA265_RS23185 at 5673.161 kb on - strand, within CA265_RS23185 at 5673.283 kb on - strand, within CA265_RS23185 at 5673.322 kb on + strand, within CA265_RS23185 at 5673.323 kb on - strand, within CA265_RS23185 at 5673.414 kb on - strand, within CA265_RS23185 at 5673.417 kb on + strand, within CA265_RS23185 at 5673.427 kb on - strand, within CA265_RS23185 at 5673.542 kb on + strand at 5673.543 kb on - strand at 5673.602 kb on + strand at 5673.603 kb on - strand at 5673.604 kb on + strand at 5673.604 kb on + strand at 5673.604 kb on + strand at 5673.605 kb on - strand at 5673.614 kb on + strand at 5673.631 kb on + strand at 5673.631 kb on + strand at 5673.631 kb on + strand at 5673.683 kb on + strand at 5673.732 kb on + strand at 5673.733 kb on - strand at 5673.757 kb on - strand at 5673.757 kb on - strand at 5673.794 kb on + strand at 5673.794 kb on + strand at 5673.794 kb on + strand at 5673.798 kb on + strand at 5673.875 kb on + strand at 5673.908 kb on + strand at 5673.970 kb on - strand at 5673.999 kb on + strand at 5673.999 kb on + strand at 5674.000 kb on - strand at 5674.012 kb on - strand at 5674.012 kb on - strand at 5674.014 kb on - strand at 5674.076 kb on + strand at 5674.077 kb on - strand at 5674.105 kb on - strand at 5674.150 kb on - strand, within CA265_RS23190 at 5674.153 kb on + strand, within CA265_RS23190 at 5674.264 kb on + strand, within CA265_RS23190 at 5674.298 kb on - strand, within CA265_RS23190 at 5674.374 kb on - strand, within CA265_RS23190 at 5674.456 kb on + strand, within CA265_RS23190 at 5674.457 kb on - strand, within CA265_RS23190 at 5674.524 kb on + strand at 5674.524 kb on + strand at 5674.532 kb on + strand at 5674.537 kb on + strand at 5674.537 kb on + strand at 5674.538 kb on - strand at 5674.560 kb on + strand at 5674.560 kb on + strand at 5674.560 kb on + strand at 5674.593 kb on + strand at 5674.594 kb on - strand at 5674.601 kb on - strand at 5674.615 kb on - strand at 5674.621 kb on + strand at 5674.689 kb on + strand, within CA265_RS23195 at 5674.690 kb on - strand, within CA265_RS23195 at 5674.702 kb on + strand, within CA265_RS23195 at 5674.703 kb on - strand, within CA265_RS23195 at 5674.788 kb on + strand, within CA265_RS23195 at 5674.788 kb on + strand, within CA265_RS23195 at 5674.788 kb on + strand, within CA265_RS23195 at 5674.788 kb on + strand, within CA265_RS23195 at 5674.898 kb on + strand, within CA265_RS23195 at 5674.899 kb on - strand, within CA265_RS23195 at 5674.899 kb on - strand, within CA265_RS23195 at 5674.899 kb on - strand, within CA265_RS23195 at 5674.899 kb on - strand, within CA265_RS23195 at 5674.899 kb on - strand, within CA265_RS23195 at 5674.944 kb on - strand, within CA265_RS23195 at 5675.019 kb on + strand at 5675.100 kb on - strand at 5675.106 kb on + strand at 5675.112 kb on + strand at 5675.194 kb on + strand, within CA265_RS23200 at 5675.195 kb on - strand, within CA265_RS23200 at 5675.212 kb on + strand, within CA265_RS23200 at 5675.213 kb on - strand, within CA265_RS23200 at 5675.213 kb on - strand, within CA265_RS23200 at 5675.246 kb on + strand, within CA265_RS23200 at 5675.247 kb on - strand, within CA265_RS23200 at 5675.247 kb on - strand, within CA265_RS23200 at 5675.343 kb on - strand, within CA265_RS23200 at 5675.344 kb on + strand, within CA265_RS23200 at 5675.348 kb on + strand, within CA265_RS23200 at 5675.352 kb on + strand, within CA265_RS23200 at 5675.353 kb on - strand, within CA265_RS23200 at 5675.380 kb on + strand, within CA265_RS23200 at 5675.380 kb on + strand, within CA265_RS23200 at 5675.380 kb on + strand, within CA265_RS23200 at 5675.388 kb on - strand at 5675.411 kb on - strand, within CA265_RS23200 at 5675.437 kb on + strand, within CA265_RS23200 at 5675.438 kb on - strand, within CA265_RS23200 at 5675.450 kb on - strand, within CA265_RS23200 at 5675.508 kb on - strand, within CA265_RS23200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,673,127 + CA265_RS23185 0.69 -0.3 5,673,128 - CA265_RS23185 0.69 +1.0 5,673,158 + CA265_RS23185 0.71 +0.3 5,673,158 + CA265_RS23185 0.71 -1.8 5,673,159 - CA265_RS23185 0.71 +0.1 5,673,159 - CA265_RS23185 0.71 -0.0 5,673,159 - CA265_RS23185 0.71 -0.4 5,673,161 - CA265_RS23185 0.71 -0.9 5,673,161 - CA265_RS23185 0.71 +1.3 5,673,283 - CA265_RS23185 0.78 +1.7 5,673,322 + CA265_RS23185 0.80 +0.7 5,673,323 - CA265_RS23185 0.80 -2.1 5,673,414 - CA265_RS23185 0.85 +0.4 5,673,417 + CA265_RS23185 0.85 +0.1 5,673,427 - CA265_RS23185 0.85 +0.4 5,673,542 + +0.3 5,673,543 - -0.5 5,673,602 + -0.4 5,673,603 - +0.5 5,673,604 + +0.7 5,673,604 + +0.1 5,673,604 + +0.2 5,673,605 - +1.5 5,673,614 + -0.6 5,673,631 + +0.5 5,673,631 + -0.4 5,673,631 + -1.1 5,673,683 + +0.0 5,673,732 + +2.3 5,673,733 - -0.0 5,673,757 - +0.5 5,673,757 - +1.2 5,673,794 + -1.3 5,673,794 + -0.6 5,673,794 + +0.1 5,673,798 + -1.1 5,673,875 + +0.1 5,673,908 + +0.5 5,673,970 - -0.2 5,673,999 + -0.9 5,673,999 + +0.4 5,674,000 - -0.3 5,674,012 - -1.4 5,674,012 - -1.2 5,674,014 - -0.1 5,674,076 + +0.7 5,674,077 - +1.7 5,674,105 - -0.9 5,674,150 - CA265_RS23190 0.19 -0.3 5,674,153 + CA265_RS23190 0.19 -1.5 5,674,264 + CA265_RS23190 0.43 +0.0 5,674,298 - CA265_RS23190 0.50 +0.3 5,674,374 - CA265_RS23190 0.66 -0.1 5,674,456 + CA265_RS23190 0.83 -0.3 5,674,457 - CA265_RS23190 0.84 -0.8 5,674,524 + -0.2 5,674,524 + +0.3 5,674,532 + -1.5 5,674,537 + -0.5 5,674,537 + +0.2 5,674,538 - -0.5 5,674,560 + +0.1 5,674,560 + +0.2 5,674,560 + -0.8 5,674,593 + -0.3 5,674,594 - -0.4 5,674,601 - +1.5 5,674,615 - +0.4 5,674,621 + -0.5 5,674,689 + CA265_RS23195 0.17 +0.2 5,674,690 - CA265_RS23195 0.17 -1.0 5,674,702 + CA265_RS23195 0.20 +0.2 5,674,703 - CA265_RS23195 0.20 -0.9 5,674,788 + CA265_RS23195 0.41 -0.6 5,674,788 + CA265_RS23195 0.41 -0.8 5,674,788 + CA265_RS23195 0.41 +0.1 5,674,788 + CA265_RS23195 0.41 -1.2 5,674,898 + CA265_RS23195 0.69 +0.6 5,674,899 - CA265_RS23195 0.69 -0.6 5,674,899 - CA265_RS23195 0.69 -0.1 5,674,899 - CA265_RS23195 0.69 +0.3 5,674,899 - CA265_RS23195 0.69 +0.3 5,674,899 - CA265_RS23195 0.69 -0.1 5,674,944 - CA265_RS23195 0.80 -0.8 5,675,019 + -0.7 5,675,100 - -0.3 5,675,106 + +0.7 5,675,112 + -0.1 5,675,194 + CA265_RS23200 0.13 +1.0 5,675,195 - CA265_RS23200 0.13 +1.0 5,675,212 + CA265_RS23200 0.15 -1.1 5,675,213 - CA265_RS23200 0.16 +0.3 5,675,213 - CA265_RS23200 0.16 -1.3 5,675,246 + CA265_RS23200 0.19 +0.1 5,675,247 - CA265_RS23200 0.20 -1.0 5,675,247 - CA265_RS23200 0.20 -0.7 5,675,343 - CA265_RS23200 0.31 +0.4 5,675,344 + CA265_RS23200 0.31 -0.2 5,675,348 + CA265_RS23200 0.31 +0.2 5,675,352 + CA265_RS23200 0.32 -0.4 5,675,353 - CA265_RS23200 0.32 -0.1 5,675,380 + CA265_RS23200 0.35 -0.1 5,675,380 + CA265_RS23200 0.35 +0.2 5,675,380 + CA265_RS23200 0.35 -0.3 5,675,388 - -0.6 5,675,411 - CA265_RS23200 0.39 -1.9 5,675,437 + CA265_RS23200 0.42 -0.1 5,675,438 - CA265_RS23200 0.42 -1.3 5,675,450 - CA265_RS23200 0.43 -0.6 5,675,508 - CA265_RS23200 0.50 +1.0
Or see this region's nucleotide sequence