Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23025 and CA265_RS23030 are separated by 388 nucleotides CA265_RS23030 and CA265_RS23035 are separated by 59 nucleotides CA265_RS23035 and CA265_RS23040 are separated by 332 nucleotides
CA265_RS23025: CA265_RS23025 - diaminopimelate epimerase, at 5,639,103 to 5,639,885
_RS23025
CA265_RS23030: CA265_RS23030 - serine protease, at 5,640,274 to 5,641,836
_RS23030
CA265_RS23035: CA265_RS23035 - four-helix bundle copper-binding protein, at 5,641,896 to 5,642,240
_RS23035
CA265_RS23040: CA265_RS23040 - hypothetical protein, at 5,642,573 to 5,643,076
_RS23040
Position (kb)
5640
5641
5642 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5639.928 kb on - strand at 5639.954 kb on - strand at 5640.005 kb on - strand at 5640.126 kb on + strand at 5640.146 kb on - strand at 5640.165 kb on - strand at 5640.190 kb on + strand at 5640.193 kb on - strand at 5640.194 kb on + strand at 5640.194 kb on + strand at 5640.195 kb on - strand at 5640.227 kb on + strand at 5640.250 kb on + strand at 5640.275 kb on + strand at 5640.277 kb on + strand at 5640.277 kb on + strand at 5640.477 kb on - strand, within CA265_RS23030 at 5640.650 kb on - strand, within CA265_RS23030 at 5640.650 kb on - strand, within CA265_RS23030 at 5640.792 kb on - strand, within CA265_RS23030 at 5640.797 kb on + strand, within CA265_RS23030 at 5640.874 kb on - strand, within CA265_RS23030 at 5641.208 kb on - strand, within CA265_RS23030 at 5641.490 kb on - strand, within CA265_RS23030 at 5641.496 kb on + strand, within CA265_RS23030 at 5641.571 kb on + strand at 5641.637 kb on + strand, within CA265_RS23030 at 5641.674 kb on + strand, within CA265_RS23030 at 5641.694 kb on + strand at 5641.754 kb on + strand at 5641.810 kb on + strand at 5641.816 kb on + strand at 5641.816 kb on + strand at 5641.817 kb on - strand at 5641.817 kb on - strand at 5641.817 kb on - strand at 5641.835 kb on - strand at 5641.853 kb on + strand at 5642.037 kb on + strand, within CA265_RS23035 at 5642.042 kb on - strand, within CA265_RS23035 at 5642.042 kb on - strand, within CA265_RS23035 at 5642.096 kb on + strand, within CA265_RS23035 at 5642.097 kb on - strand, within CA265_RS23035 at 5642.221 kb on + strand at 5642.222 kb on - strand at 5642.262 kb on - strand at 5642.274 kb on + strand at 5642.378 kb on - strand at 5642.386 kb on - strand at 5642.400 kb on + strand at 5642.442 kb on + strand at 5642.484 kb on - strand at 5642.572 kb on + strand at 5642.580 kb on + strand at 5642.580 kb on + strand at 5642.650 kb on - strand, within CA265_RS23040 at 5642.659 kb on + strand, within CA265_RS23040 at 5642.659 kb on + strand, within CA265_RS23040 at 5642.659 kb on + strand, within CA265_RS23040 at 5642.660 kb on - strand, within CA265_RS23040 at 5642.663 kb on + strand, within CA265_RS23040 at 5642.692 kb on - strand, within CA265_RS23040 at 5642.692 kb on - strand, within CA265_RS23040 at 5642.692 kb on - strand, within CA265_RS23040 at 5642.777 kb on + strand, within CA265_RS23040 at 5642.812 kb on + strand, within CA265_RS23040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,639,928 - -0.1 5,639,954 - -0.3 5,640,005 - -1.7 5,640,126 + -1.2 5,640,146 - -1.3 5,640,165 - -1.7 5,640,190 + +0.0 5,640,193 - -2.6 5,640,194 + -1.1 5,640,194 + -0.2 5,640,195 - -2.1 5,640,227 + +0.0 5,640,250 + +0.4 5,640,275 + +0.9 5,640,277 + -1.3 5,640,277 + +0.4 5,640,477 - CA265_RS23030 0.13 -0.3 5,640,650 - CA265_RS23030 0.24 -2.0 5,640,650 - CA265_RS23030 0.24 -2.5 5,640,792 - CA265_RS23030 0.33 -0.3 5,640,797 + CA265_RS23030 0.33 -2.6 5,640,874 - CA265_RS23030 0.38 -0.6 5,641,208 - CA265_RS23030 0.60 -2.8 5,641,490 - CA265_RS23030 0.78 -1.3 5,641,496 + CA265_RS23030 0.78 -0.6 5,641,571 + -0.6 5,641,637 + CA265_RS23030 0.87 -0.6 5,641,674 + CA265_RS23030 0.90 +0.8 5,641,694 + -1.4 5,641,754 + -0.9 5,641,810 + -0.3 5,641,816 + -0.6 5,641,816 + -0.6 5,641,817 - -2.2 5,641,817 - +0.2 5,641,817 - -0.8 5,641,835 - -1.2 5,641,853 + +0.6 5,642,037 + CA265_RS23035 0.41 -0.0 5,642,042 - CA265_RS23035 0.42 -0.3 5,642,042 - CA265_RS23035 0.42 -0.4 5,642,096 + CA265_RS23035 0.58 -0.5 5,642,097 - CA265_RS23035 0.58 +1.0 5,642,221 + -0.3 5,642,222 - -0.6 5,642,262 - -1.1 5,642,274 + +0.2 5,642,378 - +0.5 5,642,386 - -0.7 5,642,400 + +1.7 5,642,442 + +0.2 5,642,484 - -0.6 5,642,572 + +0.0 5,642,580 + -0.1 5,642,580 + +0.4 5,642,650 - CA265_RS23040 0.15 +1.7 5,642,659 + CA265_RS23040 0.17 +0.5 5,642,659 + CA265_RS23040 0.17 +0.1 5,642,659 + CA265_RS23040 0.17 +0.3 5,642,660 - CA265_RS23040 0.17 -0.3 5,642,663 + CA265_RS23040 0.18 -0.8 5,642,692 - CA265_RS23040 0.24 +1.7 5,642,692 - CA265_RS23040 0.24 -0.1 5,642,692 - CA265_RS23040 0.24 +0.3 5,642,777 + CA265_RS23040 0.40 +0.6 5,642,812 + CA265_RS23040 0.47 +0.2
Or see this region's nucleotide sequence