Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22890 and CA265_RS22895 are separated by 50 nucleotides CA265_RS22895 and CA265_RS22900 are separated by 233 nucleotides CA265_RS22900 and CA265_RS22905 are separated by 339 nucleotides
CA265_RS22890: CA265_RS22890 - anthranilate synthase component I, at 5,607,236 to 5,608,636
_RS22890
CA265_RS22895: CA265_RS22895 - aminodeoxychorismate/anthranilate synthase component II, at 5,608,687 to 5,609,283
_RS22895
CA265_RS22900: CA265_RS22900 - indole-3-glycerol phosphate synthase, at 5,609,517 to 5,610,299
_RS22900
CA265_RS22905: CA265_RS22905 - GTPase HflX, at 5,610,639 to 5,611,829
_RS22905
Position (kb)
5609
5610
5611 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5608.645 kb on + strand at 5608.678 kb on - strand at 5608.683 kb on + strand at 5608.695 kb on + strand at 5608.906 kb on + strand, within CA265_RS22895 at 5608.907 kb on - strand, within CA265_RS22895 at 5608.940 kb on + strand, within CA265_RS22895 at 5608.983 kb on - strand, within CA265_RS22895 at 5609.073 kb on - strand, within CA265_RS22895 at 5609.087 kb on - strand, within CA265_RS22895 at 5609.152 kb on - strand, within CA265_RS22895 at 5609.163 kb on + strand, within CA265_RS22895 at 5609.187 kb on - strand, within CA265_RS22895 at 5609.202 kb on - strand, within CA265_RS22895 at 5609.272 kb on - strand at 5609.363 kb on + strand at 5609.364 kb on - strand at 5609.368 kb on - strand at 5609.368 kb on - strand at 5609.370 kb on - strand at 5609.407 kb on - strand at 5609.629 kb on - strand, within CA265_RS22900 at 5609.714 kb on + strand, within CA265_RS22900 at 5609.751 kb on + strand, within CA265_RS22900 at 5609.752 kb on - strand, within CA265_RS22900 at 5609.850 kb on - strand, within CA265_RS22900 at 5609.874 kb on - strand, within CA265_RS22900 at 5609.944 kb on + strand, within CA265_RS22900 at 5610.182 kb on + strand, within CA265_RS22900 at 5610.295 kb on + strand at 5610.296 kb on - strand at 5610.296 kb on - strand at 5610.316 kb on + strand at 5610.362 kb on - strand at 5610.385 kb on + strand at 5610.456 kb on + strand at 5610.508 kb on - strand at 5610.529 kb on + strand at 5610.636 kb on + strand at 5610.742 kb on + strand at 5610.742 kb on + strand at 5610.764 kb on - strand, within CA265_RS22905 at 5610.859 kb on + strand, within CA265_RS22905 at 5611.142 kb on - strand, within CA265_RS22905 at 5611.265 kb on - strand, within CA265_RS22905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,608,645 + +1.4 5,608,678 - -0.7 5,608,683 + -0.2 5,608,695 + +0.3 5,608,906 + CA265_RS22895 0.37 -1.2 5,608,907 - CA265_RS22895 0.37 -0.2 5,608,940 + CA265_RS22895 0.42 -0.2 5,608,983 - CA265_RS22895 0.50 -0.8 5,609,073 - CA265_RS22895 0.65 +0.0 5,609,087 - CA265_RS22895 0.67 -2.1 5,609,152 - CA265_RS22895 0.78 +1.0 5,609,163 + CA265_RS22895 0.80 -2.1 5,609,187 - CA265_RS22895 0.84 +0.3 5,609,202 - CA265_RS22895 0.86 +0.4 5,609,272 - -0.4 5,609,363 + +0.7 5,609,364 - -0.2 5,609,368 - -0.6 5,609,368 - -0.1 5,609,370 - +0.2 5,609,407 - -0.0 5,609,629 - CA265_RS22900 0.14 +0.2 5,609,714 + CA265_RS22900 0.25 +0.8 5,609,751 + CA265_RS22900 0.30 -0.2 5,609,752 - CA265_RS22900 0.30 -2.2 5,609,850 - CA265_RS22900 0.43 -1.4 5,609,874 - CA265_RS22900 0.46 +0.8 5,609,944 + CA265_RS22900 0.55 -1.2 5,610,182 + CA265_RS22900 0.85 +1.8 5,610,295 + -0.9 5,610,296 - +0.3 5,610,296 - -0.5 5,610,316 + +0.8 5,610,362 - +1.6 5,610,385 + -0.3 5,610,456 + -0.2 5,610,508 - +0.6 5,610,529 + +1.0 5,610,636 + +0.1 5,610,742 + +0.9 5,610,742 + -0.8 5,610,764 - CA265_RS22905 0.10 -0.5 5,610,859 + CA265_RS22905 0.18 +1.8 5,611,142 - CA265_RS22905 0.42 -0.2 5,611,265 - CA265_RS22905 0.53 +1.5
Or see this region's nucleotide sequence