Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22880 and CA265_RS22885 are separated by 112 nucleotides CA265_RS22885 and CA265_RS22890 are separated by 351 nucleotides CA265_RS22890 and CA265_RS22895 are separated by 50 nucleotides CA265_RS22895 and CA265_RS22900 are separated by 233 nucleotides
CA265_RS22880: CA265_RS22880 - amidohydrolase, at 5,603,308 to 5,606,328
_RS22880
CA265_RS22885: CA265_RS22885 - cytochrome B, at 5,606,441 to 5,606,884
_RS22885
CA265_RS22890: CA265_RS22890 - anthranilate synthase component I, at 5,607,236 to 5,608,636
_RS22890
CA265_RS22895: CA265_RS22895 - aminodeoxychorismate/anthranilate synthase component II, at 5,608,687 to 5,609,283
_RS22895
CA265_RS22900: CA265_RS22900 - indole-3-glycerol phosphate synthase, at 5,609,517 to 5,610,299
_RS22900
Position (kb)
5607
5608
5609 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5606.331 kb on + strand at 5606.406 kb on + strand at 5606.406 kb on + strand at 5606.406 kb on + strand at 5606.430 kb on - strand at 5606.490 kb on + strand, within CA265_RS22885 at 5606.504 kb on - strand, within CA265_RS22885 at 5606.512 kb on - strand, within CA265_RS22885 at 5606.581 kb on + strand, within CA265_RS22885 at 5606.668 kb on + strand, within CA265_RS22885 at 5606.696 kb on - strand, within CA265_RS22885 at 5606.728 kb on + strand, within CA265_RS22885 at 5606.729 kb on - strand, within CA265_RS22885 at 5606.846 kb on - strand at 5606.846 kb on - strand at 5606.883 kb on - strand at 5606.940 kb on - strand at 5606.942 kb on - strand at 5606.984 kb on - strand at 5607.021 kb on - strand at 5607.047 kb on + strand at 5607.047 kb on + strand at 5607.047 kb on + strand at 5607.048 kb on - strand at 5607.048 kb on - strand at 5607.051 kb on + strand at 5607.051 kb on + strand at 5607.051 kb on + strand at 5607.051 kb on + strand at 5607.052 kb on - strand at 5607.052 kb on - strand at 5607.070 kb on - strand at 5607.132 kb on + strand at 5607.206 kb on + strand at 5607.213 kb on + strand at 5607.303 kb on - strand at 5607.441 kb on - strand, within CA265_RS22890 at 5607.588 kb on + strand, within CA265_RS22890 at 5607.605 kb on - strand, within CA265_RS22890 at 5607.676 kb on + strand, within CA265_RS22890 at 5607.794 kb on + strand, within CA265_RS22890 at 5607.795 kb on - strand, within CA265_RS22890 at 5607.878 kb on - strand, within CA265_RS22890 at 5607.878 kb on - strand, within CA265_RS22890 at 5607.878 kb on - strand, within CA265_RS22890 at 5607.956 kb on + strand, within CA265_RS22890 at 5607.956 kb on + strand, within CA265_RS22890 at 5608.181 kb on - strand, within CA265_RS22890 at 5608.247 kb on - strand, within CA265_RS22890 at 5608.262 kb on + strand, within CA265_RS22890 at 5608.347 kb on - strand, within CA265_RS22890 at 5608.357 kb on + strand, within CA265_RS22890 at 5608.450 kb on + strand, within CA265_RS22890 at 5608.645 kb on + strand at 5608.678 kb on - strand at 5608.683 kb on + strand at 5608.695 kb on + strand at 5608.906 kb on + strand, within CA265_RS22895 at 5608.907 kb on - strand, within CA265_RS22895 at 5608.940 kb on + strand, within CA265_RS22895 at 5608.983 kb on - strand, within CA265_RS22895 at 5609.073 kb on - strand, within CA265_RS22895 at 5609.087 kb on - strand, within CA265_RS22895 at 5609.152 kb on - strand, within CA265_RS22895 at 5609.163 kb on + strand, within CA265_RS22895 at 5609.187 kb on - strand, within CA265_RS22895 at 5609.202 kb on - strand, within CA265_RS22895 at 5609.272 kb on - strand at 5609.363 kb on + strand at 5609.364 kb on - strand at 5609.368 kb on - strand at 5609.368 kb on - strand at 5609.370 kb on - strand at 5609.407 kb on - strand at 5609.629 kb on - strand, within CA265_RS22900
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,606,331 + -0.3 5,606,406 + -0.9 5,606,406 + +0.2 5,606,406 + -0.6 5,606,430 - +0.2 5,606,490 + CA265_RS22885 0.11 -0.4 5,606,504 - CA265_RS22885 0.14 -1.0 5,606,512 - CA265_RS22885 0.16 +1.6 5,606,581 + CA265_RS22885 0.32 -0.2 5,606,668 + CA265_RS22885 0.51 +0.0 5,606,696 - CA265_RS22885 0.57 +0.0 5,606,728 + CA265_RS22885 0.65 -0.0 5,606,729 - CA265_RS22885 0.65 +0.9 5,606,846 - +0.5 5,606,846 - +0.8 5,606,883 - +0.9 5,606,940 - -0.9 5,606,942 - +0.9 5,606,984 - +0.4 5,607,021 - -0.4 5,607,047 + -1.2 5,607,047 + +0.7 5,607,047 + -0.2 5,607,048 - -0.1 5,607,048 - +0.4 5,607,051 + -0.7 5,607,051 + +0.4 5,607,051 + -0.0 5,607,051 + -0.2 5,607,052 - +0.5 5,607,052 - +0.3 5,607,070 - -0.6 5,607,132 + -0.4 5,607,206 + -0.1 5,607,213 + +0.0 5,607,303 - +0.3 5,607,441 - CA265_RS22890 0.15 -0.6 5,607,588 + CA265_RS22890 0.25 +0.8 5,607,605 - CA265_RS22890 0.26 +0.9 5,607,676 + CA265_RS22890 0.31 -0.4 5,607,794 + CA265_RS22890 0.40 +1.8 5,607,795 - CA265_RS22890 0.40 -0.2 5,607,878 - CA265_RS22890 0.46 -2.6 5,607,878 - CA265_RS22890 0.46 -0.8 5,607,878 - CA265_RS22890 0.46 -1.1 5,607,956 + CA265_RS22890 0.51 +0.4 5,607,956 + CA265_RS22890 0.51 -1.5 5,608,181 - CA265_RS22890 0.67 -1.0 5,608,247 - CA265_RS22890 0.72 +0.4 5,608,262 + CA265_RS22890 0.73 -1.6 5,608,347 - CA265_RS22890 0.79 -2.6 5,608,357 + CA265_RS22890 0.80 -0.7 5,608,450 + CA265_RS22890 0.87 +0.1 5,608,645 + +1.4 5,608,678 - -0.7 5,608,683 + -0.2 5,608,695 + +0.3 5,608,906 + CA265_RS22895 0.37 -1.2 5,608,907 - CA265_RS22895 0.37 -0.2 5,608,940 + CA265_RS22895 0.42 -0.2 5,608,983 - CA265_RS22895 0.50 -0.8 5,609,073 - CA265_RS22895 0.65 +0.0 5,609,087 - CA265_RS22895 0.67 -2.1 5,609,152 - CA265_RS22895 0.78 +1.0 5,609,163 + CA265_RS22895 0.80 -2.1 5,609,187 - CA265_RS22895 0.84 +0.3 5,609,202 - CA265_RS22895 0.86 +0.4 5,609,272 - -0.4 5,609,363 + +0.7 5,609,364 - -0.2 5,609,368 - -0.6 5,609,368 - -0.1 5,609,370 - +0.2 5,609,407 - -0.0 5,609,629 - CA265_RS22900 0.14 +0.2
Or see this region's nucleotide sequence