Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22775 and CA265_RS22780 are separated by 15 nucleotides CA265_RS22780 and CA265_RS22785 are separated by 15 nucleotides CA265_RS22785 and CA265_RS22790 overlap by 8 nucleotides CA265_RS22790 and CA265_RS22795 are separated by 4 nucleotides
CA265_RS22775: CA265_RS22775 - glycosyl hydrolase, at 5,572,769 to 5,573,884
_RS22775
CA265_RS22780: CA265_RS22780 - hypothetical protein, at 5,573,900 to 5,574,799
_RS22780
CA265_RS22785: CA265_RS22785 - hypothetical protein, at 5,574,815 to 5,575,822
_RS22785
CA265_RS22790: CA265_RS22790 - hypothetical protein, at 5,575,815 to 5,576,648
_RS22790
CA265_RS22795: CA265_RS22795 - antibiotic ABC transporter ATP-binding protein, at 5,576,653 to 5,578,470
_RS22795
Position (kb) 5574
5575
5576 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5573.834 kb on + strand at 5573.839 kb on - strand at 5573.839 kb on - strand at 5573.901 kb on + strand at 5573.901 kb on + strand at 5573.902 kb on - strand at 5574.817 kb on - strand at 5574.885 kb on + strand at 5575.004 kb on - strand, within CA265_RS22785 at 5575.058 kb on - strand, within CA265_RS22785 at 5575.113 kb on + strand, within CA265_RS22785 at 5575.114 kb on - strand, within CA265_RS22785 at 5575.167 kb on - strand, within CA265_RS22785 at 5575.449 kb on + strand, within CA265_RS22785 at 5575.508 kb on + strand, within CA265_RS22785 at 5575.622 kb on + strand, within CA265_RS22785 at 5575.623 kb on - strand, within CA265_RS22785 at 5575.647 kb on + strand, within CA265_RS22785 at 5575.669 kb on + strand, within CA265_RS22785 at 5575.702 kb on + strand, within CA265_RS22785 at 5575.702 kb on + strand, within CA265_RS22785 at 5575.702 kb on + strand, within CA265_RS22785 at 5575.746 kb on - strand at 5575.790 kb on - strand at 5575.817 kb on - strand at 5575.830 kb on - strand at 5575.837 kb on - strand at 5575.837 kb on - strand at 5575.840 kb on - strand at 5575.870 kb on + strand at 5575.880 kb on + strand at 5575.880 kb on + strand at 5575.943 kb on - strand, within CA265_RS22790 at 5575.943 kb on - strand, within CA265_RS22790 at 5575.994 kb on - strand, within CA265_RS22790 at 5576.016 kb on + strand, within CA265_RS22790 at 5576.061 kb on - strand, within CA265_RS22790 at 5576.064 kb on - strand, within CA265_RS22790 at 5576.067 kb on - strand, within CA265_RS22790 at 5576.094 kb on + strand, within CA265_RS22790 at 5576.243 kb on + strand, within CA265_RS22790 at 5576.383 kb on - strand, within CA265_RS22790 at 5576.388 kb on - strand, within CA265_RS22790 at 5576.391 kb on - strand, within CA265_RS22790 at 5576.411 kb on + strand, within CA265_RS22790 at 5576.413 kb on + strand, within CA265_RS22790 at 5576.413 kb on + strand, within CA265_RS22790 at 5576.414 kb on - strand, within CA265_RS22790 at 5576.498 kb on - strand, within CA265_RS22790 at 5576.529 kb on - strand, within CA265_RS22790 at 5576.579 kb on + strand at 5576.580 kb on - strand at 5576.585 kb on + strand at 5576.585 kb on + strand at 5576.585 kb on + strand at 5576.596 kb on + strand at 5576.596 kb on + strand at 5576.597 kb on - strand at 5576.603 kb on - strand at 5576.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,573,834 + -0.5 5,573,839 - -1.1 5,573,839 - -1.8 5,573,901 + -0.1 5,573,901 + +0.5 5,573,902 - +0.2 5,574,817 - -0.8 5,574,885 + -1.0 5,575,004 - CA265_RS22785 0.19 -1.2 5,575,058 - CA265_RS22785 0.24 +0.0 5,575,113 + CA265_RS22785 0.30 -0.4 5,575,114 - CA265_RS22785 0.30 -2.5 5,575,167 - CA265_RS22785 0.35 -2.0 5,575,449 + CA265_RS22785 0.63 +0.2 5,575,508 + CA265_RS22785 0.69 -2.1 5,575,622 + CA265_RS22785 0.80 -2.9 5,575,623 - CA265_RS22785 0.80 -0.6 5,575,647 + CA265_RS22785 0.83 -1.1 5,575,669 + CA265_RS22785 0.85 -1.1 5,575,702 + CA265_RS22785 0.88 -1.2 5,575,702 + CA265_RS22785 0.88 -0.7 5,575,702 + CA265_RS22785 0.88 -2.3 5,575,746 - -1.8 5,575,790 - -1.5 5,575,817 - -2.7 5,575,830 - +1.2 5,575,837 - -0.2 5,575,837 - -1.1 5,575,840 - -0.2 5,575,870 + +0.4 5,575,880 + -0.4 5,575,880 + -0.4 5,575,943 - CA265_RS22790 0.15 -0.3 5,575,943 - CA265_RS22790 0.15 -0.5 5,575,994 - CA265_RS22790 0.21 -2.4 5,576,016 + CA265_RS22790 0.24 +1.0 5,576,061 - CA265_RS22790 0.29 -0.4 5,576,064 - CA265_RS22790 0.30 -0.4 5,576,067 - CA265_RS22790 0.30 +0.2 5,576,094 + CA265_RS22790 0.33 +0.4 5,576,243 + CA265_RS22790 0.51 -0.5 5,576,383 - CA265_RS22790 0.68 -1.7 5,576,388 - CA265_RS22790 0.69 -0.6 5,576,391 - CA265_RS22790 0.69 +0.1 5,576,411 + CA265_RS22790 0.71 +0.2 5,576,413 + CA265_RS22790 0.72 -0.2 5,576,413 + CA265_RS22790 0.72 -0.2 5,576,414 - CA265_RS22790 0.72 -0.4 5,576,498 - CA265_RS22790 0.82 -1.2 5,576,529 - CA265_RS22790 0.86 +0.3 5,576,579 + -1.2 5,576,580 - -0.1 5,576,585 + -2.5 5,576,585 + -0.4 5,576,585 + -0.9 5,576,596 + -1.5 5,576,596 + -0.8 5,576,597 - +0.4 5,576,603 - -0.2 5,576,643 - +0.3
Or see this region's nucleotide sequence