Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22440 and CA265_RS22445 are separated by 126 nucleotides CA265_RS22445 and CA265_RS22450 are separated by 4 nucleotides CA265_RS22450 and CA265_RS22455 are separated by 18 nucleotides CA265_RS22455 and CA265_RS22460 are separated by 3 nucleotides
CA265_RS22440: CA265_RS22440 - alpha/beta hydrolase, at 5,505,804 to 5,506,790
_RS22440
CA265_RS22445: CA265_RS22445 - hypothetical protein, at 5,506,917 to 5,507,756
_RS22445
CA265_RS22450: CA265_RS22450 - GntR family transcriptional regulator, at 5,507,761 to 5,508,135
_RS22450
CA265_RS22455: CA265_RS22455 - hypothetical protein, at 5,508,154 to 5,508,966
_RS22455
CA265_RS22460: CA265_RS22460 - ABC transporter ATP-binding protein, at 5,508,970 to 5,509,797
_RS22460
Position (kb)
5507
5508
5509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5506.770 kb on + strand at 5506.770 kb on + strand at 5506.771 kb on - strand at 5506.834 kb on + strand at 5506.849 kb on + strand at 5506.849 kb on + strand at 5506.850 kb on - strand at 5506.850 kb on - strand at 5506.854 kb on - strand at 5507.043 kb on - strand, within CA265_RS22445 at 5507.094 kb on + strand, within CA265_RS22445 at 5507.094 kb on + strand, within CA265_RS22445 at 5507.118 kb on - strand, within CA265_RS22445 at 5507.283 kb on + strand, within CA265_RS22445 at 5507.283 kb on + strand, within CA265_RS22445 at 5507.305 kb on - strand, within CA265_RS22445 at 5507.346 kb on - strand, within CA265_RS22445 at 5507.356 kb on + strand, within CA265_RS22445 at 5507.357 kb on - strand, within CA265_RS22445 at 5507.358 kb on + strand, within CA265_RS22445 at 5507.358 kb on + strand, within CA265_RS22445 at 5507.359 kb on - strand, within CA265_RS22445 at 5507.359 kb on - strand, within CA265_RS22445 at 5507.371 kb on - strand, within CA265_RS22445 at 5507.371 kb on - strand, within CA265_RS22445 at 5507.438 kb on + strand, within CA265_RS22445 at 5507.439 kb on - strand, within CA265_RS22445 at 5507.439 kb on - strand, within CA265_RS22445 at 5507.443 kb on - strand, within CA265_RS22445 at 5507.547 kb on - strand, within CA265_RS22445 at 5507.646 kb on - strand, within CA265_RS22445 at 5507.666 kb on - strand, within CA265_RS22445 at 5507.671 kb on - strand, within CA265_RS22445 at 5507.695 kb on - strand at 5507.712 kb on + strand at 5507.737 kb on - strand at 5507.759 kb on + strand at 5507.826 kb on + strand at 5507.839 kb on - strand, within CA265_RS22450 at 5507.901 kb on + strand, within CA265_RS22450 at 5507.902 kb on - strand, within CA265_RS22450 at 5507.902 kb on - strand, within CA265_RS22450 at 5507.905 kb on + strand, within CA265_RS22450 at 5508.089 kb on + strand, within CA265_RS22450 at 5508.098 kb on - strand at 5508.107 kb on + strand at 5508.107 kb on + strand at 5508.110 kb on - strand at 5508.139 kb on + strand at 5508.139 kb on + strand at 5508.142 kb on - strand at 5508.183 kb on - strand at 5508.188 kb on - strand at 5508.199 kb on - strand at 5508.201 kb on + strand at 5508.268 kb on - strand, within CA265_RS22455 at 5508.270 kb on + strand, within CA265_RS22455 at 5508.272 kb on + strand, within CA265_RS22455 at 5508.273 kb on - strand, within CA265_RS22455 at 5508.371 kb on + strand, within CA265_RS22455 at 5508.497 kb on - strand, within CA265_RS22455 at 5508.497 kb on - strand, within CA265_RS22455 at 5508.518 kb on + strand, within CA265_RS22455 at 5508.581 kb on - strand, within CA265_RS22455 at 5508.600 kb on - strand, within CA265_RS22455 at 5508.618 kb on + strand, within CA265_RS22455 at 5508.625 kb on - strand, within CA265_RS22455 at 5508.674 kb on - strand, within CA265_RS22455 at 5508.711 kb on + strand at 5508.743 kb on - strand, within CA265_RS22455 at 5508.743 kb on - strand, within CA265_RS22455 at 5508.826 kb on - strand, within CA265_RS22455 at 5508.861 kb on + strand, within CA265_RS22455 at 5508.866 kb on - strand, within CA265_RS22455 at 5508.874 kb on + strand, within CA265_RS22455 at 5508.874 kb on + strand, within CA265_RS22455 at 5508.948 kb on + strand at 5508.952 kb on - strand at 5508.952 kb on - strand at 5508.971 kb on + strand at 5508.980 kb on - strand at 5509.079 kb on + strand, within CA265_RS22460 at 5509.079 kb on + strand, within CA265_RS22460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 5,506,770 + +0.1 5,506,770 + -0.9 5,506,771 - +0.3 5,506,834 + -0.1 5,506,849 + +0.2 5,506,849 + +0.8 5,506,850 - -1.3 5,506,850 - -0.1 5,506,854 - -0.6 5,507,043 - CA265_RS22445 0.15 +0.0 5,507,094 + CA265_RS22445 0.21 +0.1 5,507,094 + CA265_RS22445 0.21 +0.3 5,507,118 - CA265_RS22445 0.24 +2.4 5,507,283 + CA265_RS22445 0.44 +0.5 5,507,283 + CA265_RS22445 0.44 +0.5 5,507,305 - CA265_RS22445 0.46 -0.5 5,507,346 - CA265_RS22445 0.51 -0.3 5,507,356 + CA265_RS22445 0.52 +0.7 5,507,357 - CA265_RS22445 0.52 -0.2 5,507,358 + CA265_RS22445 0.53 -0.2 5,507,358 + CA265_RS22445 0.53 +0.2 5,507,359 - CA265_RS22445 0.53 +0.5 5,507,359 - CA265_RS22445 0.53 +0.2 5,507,371 - CA265_RS22445 0.54 +0.1 5,507,371 - CA265_RS22445 0.54 -0.2 5,507,438 + CA265_RS22445 0.62 +0.1 5,507,439 - CA265_RS22445 0.62 +0.6 5,507,439 - CA265_RS22445 0.62 +0.0 5,507,443 - CA265_RS22445 0.63 -0.3 5,507,547 - CA265_RS22445 0.75 -0.1 5,507,646 - CA265_RS22445 0.87 -0.1 5,507,666 - CA265_RS22445 0.89 +0.6 5,507,671 - CA265_RS22445 0.90 +0.2 5,507,695 - +0.0 5,507,712 + -0.2 5,507,737 - +0.4 5,507,759 + +0.1 5,507,826 + -0.2 5,507,839 - CA265_RS22450 0.21 -0.5 5,507,901 + CA265_RS22450 0.37 -1.5 5,507,902 - CA265_RS22450 0.38 -2.2 5,507,902 - CA265_RS22450 0.38 -0.8 5,507,905 + CA265_RS22450 0.38 -0.5 5,508,089 + CA265_RS22450 0.87 -0.8 5,508,098 - -0.1 5,508,107 + -0.2 5,508,107 + -0.2 5,508,110 - -1.9 5,508,139 + -0.8 5,508,139 + +0.1 5,508,142 - +0.6 5,508,183 - -0.2 5,508,188 - -0.8 5,508,199 - +0.8 5,508,201 + +0.3 5,508,268 - CA265_RS22455 0.14 +0.0 5,508,270 + CA265_RS22455 0.14 -0.0 5,508,272 + CA265_RS22455 0.15 -0.0 5,508,273 - CA265_RS22455 0.15 +0.3 5,508,371 + CA265_RS22455 0.27 -0.3 5,508,497 - CA265_RS22455 0.42 -0.6 5,508,497 - CA265_RS22455 0.42 -1.1 5,508,518 + CA265_RS22455 0.45 +0.1 5,508,581 - CA265_RS22455 0.53 -1.5 5,508,600 - CA265_RS22455 0.55 -0.5 5,508,618 + CA265_RS22455 0.57 +1.2 5,508,625 - CA265_RS22455 0.58 +1.4 5,508,674 - CA265_RS22455 0.64 +0.8 5,508,711 + +1.8 5,508,743 - CA265_RS22455 0.72 +0.0 5,508,743 - CA265_RS22455 0.72 +0.3 5,508,826 - CA265_RS22455 0.83 +0.3 5,508,861 + CA265_RS22455 0.87 -0.5 5,508,866 - CA265_RS22455 0.88 -0.1 5,508,874 + CA265_RS22455 0.89 -0.3 5,508,874 + CA265_RS22455 0.89 +0.6 5,508,948 + +0.5 5,508,952 - +0.2 5,508,952 - -0.8 5,508,971 + -0.3 5,508,980 - -0.0 5,509,079 + CA265_RS22460 0.13 +0.2 5,509,079 + CA265_RS22460 0.13 -0.2
Or see this region's nucleotide sequence