Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20190 and CA265_RS20195 are separated by 255 nucleotides CA265_RS20195 and CA265_RS20200 are separated by 177 nucleotides CA265_RS20200 and CA265_RS20205 are separated by 250 nucleotides
CA265_RS20190: CA265_RS20190 - thiamine biosynthesis protein ThiF, at 4,990,061 to 4,991,098
_RS20190
CA265_RS20195: CA265_RS20195 - hypothetical protein, at 4,991,354 to 4,991,905
_RS20195
CA265_RS20200: CA265_RS20200 - nuclease, at 4,992,083 to 4,992,595
_RS20200
CA265_RS20205: CA265_RS20205 - very short patch repair endonuclease, at 4,992,846 to 4,993,349
_RS20205
Position (kb)
4991
4992 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4990.451 kb on - strand, within CA265_RS20190 at 4990.460 kb on + strand, within CA265_RS20190 at 4990.498 kb on - strand, within CA265_RS20190 at 4990.519 kb on - strand, within CA265_RS20190 at 4990.542 kb on + strand, within CA265_RS20190 at 4990.546 kb on + strand, within CA265_RS20190 at 4990.547 kb on - strand, within CA265_RS20190 at 4990.569 kb on - strand, within CA265_RS20190 at 4990.569 kb on - strand, within CA265_RS20190 at 4990.571 kb on - strand, within CA265_RS20190 at 4990.626 kb on + strand, within CA265_RS20190 at 4990.683 kb on + strand, within CA265_RS20190 at 4990.822 kb on + strand, within CA265_RS20190 at 4990.838 kb on - strand, within CA265_RS20190 at 4990.852 kb on + strand, within CA265_RS20190 at 4990.927 kb on + strand, within CA265_RS20190 at 4991.075 kb on + strand at 4991.075 kb on + strand at 4991.075 kb on + strand at 4991.075 kb on + strand at 4991.075 kb on + strand at 4991.075 kb on + strand at 4991.075 kb on + strand at 4991.193 kb on - strand at 4991.326 kb on - strand at 4991.344 kb on + strand at 4991.348 kb on + strand at 4991.393 kb on - strand at 4991.519 kb on - strand, within CA265_RS20195 at 4991.540 kb on + strand, within CA265_RS20195 at 4991.561 kb on - strand, within CA265_RS20195 at 4991.561 kb on - strand, within CA265_RS20195 at 4991.836 kb on + strand, within CA265_RS20195 at 4991.864 kb on + strand at 4991.909 kb on + strand at 4991.988 kb on - strand at 4991.991 kb on - strand at 4991.995 kb on + strand at 4992.077 kb on - strand at 4992.077 kb on - strand at 4992.078 kb on + strand at 4992.222 kb on + strand, within CA265_RS20200 at 4992.351 kb on + strand, within CA265_RS20200 at 4992.352 kb on - strand, within CA265_RS20200 at 4992.384 kb on + strand, within CA265_RS20200 at 4992.407 kb on - strand, within CA265_RS20200 at 4992.598 kb on + strand at 4992.606 kb on + strand at 4992.611 kb on + strand at 4992.615 kb on + strand at 4992.616 kb on - strand at 4992.656 kb on + strand at 4992.657 kb on - strand at 4992.682 kb on - strand at 4992.691 kb on - strand at 4992.691 kb on - strand at 4992.802 kb on - strand at 4992.840 kb on + strand at 4992.845 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,990,451 - CA265_RS20190 0.38 -1.3 4,990,460 + CA265_RS20190 0.38 -0.5 4,990,498 - CA265_RS20190 0.42 +0.3 4,990,519 - CA265_RS20190 0.44 +0.0 4,990,542 + CA265_RS20190 0.46 -0.1 4,990,546 + CA265_RS20190 0.47 -0.4 4,990,547 - CA265_RS20190 0.47 +0.5 4,990,569 - CA265_RS20190 0.49 +0.1 4,990,569 - CA265_RS20190 0.49 +0.6 4,990,571 - CA265_RS20190 0.49 -0.4 4,990,626 + CA265_RS20190 0.54 -0.8 4,990,683 + CA265_RS20190 0.60 +0.3 4,990,822 + CA265_RS20190 0.73 -0.1 4,990,838 - CA265_RS20190 0.75 +0.2 4,990,852 + CA265_RS20190 0.76 -0.9 4,990,927 + CA265_RS20190 0.83 -1.4 4,991,075 + +0.1 4,991,075 + -0.4 4,991,075 + +0.5 4,991,075 + +0.4 4,991,075 + +0.3 4,991,075 + +1.6 4,991,075 + +0.3 4,991,193 - -0.2 4,991,326 - +1.2 4,991,344 + -0.5 4,991,348 + +0.6 4,991,393 - +0.8 4,991,519 - CA265_RS20195 0.30 -0.8 4,991,540 + CA265_RS20195 0.34 +0.0 4,991,561 - CA265_RS20195 0.38 -2.1 4,991,561 - CA265_RS20195 0.38 +2.4 4,991,836 + CA265_RS20195 0.87 -1.2 4,991,864 + -0.4 4,991,909 + -0.2 4,991,988 - -0.2 4,991,991 - -1.0 4,991,995 + +1.2 4,992,077 - -0.9 4,992,077 - +1.6 4,992,078 + +0.1 4,992,222 + CA265_RS20200 0.27 +0.0 4,992,351 + CA265_RS20200 0.52 +0.2 4,992,352 - CA265_RS20200 0.52 +0.8 4,992,384 + CA265_RS20200 0.59 +0.2 4,992,407 - CA265_RS20200 0.63 -2.0 4,992,598 + -0.6 4,992,606 + +0.6 4,992,611 + +0.4 4,992,615 + +0.0 4,992,616 - +0.8 4,992,656 + -0.2 4,992,657 - +0.5 4,992,682 - +0.8 4,992,691 - +0.3 4,992,691 - -0.9 4,992,802 - +0.1 4,992,840 + +0.3 4,992,845 + +0.6
Or see this region's nucleotide sequence