Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19405 and CA265_RS19410 are separated by 0 nucleotides CA265_RS19410 and CA265_RS19415 are separated by 100 nucleotides CA265_RS19415 and CA265_RS19420 are separated by 130 nucleotides CA265_RS19420 and CA265_RS19425 are separated by 55 nucleotides
CA265_RS19405: CA265_RS19405 - transcriptional regulator, at 4,817,724 to 4,818,233
_RS19405
CA265_RS19410: CA265_RS19410 - hypothetical protein, at 4,818,234 to 4,818,683
_RS19410
CA265_RS19415: CA265_RS19415 - DUF4834 domain-containing protein, at 4,818,784 to 4,819,032
_RS19415
CA265_RS19420: CA265_RS19420 - hypothetical protein, at 4,819,163 to 4,819,360
_RS19420
CA265_RS19425: CA265_RS19425 - nematoblast specific protein, at 4,819,416 to 4,820,708
_RS19425
Position (kb)
4818
4819
4820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4817.812 kb on + strand, within CA265_RS19405 at 4817.812 kb on + strand, within CA265_RS19405 at 4817.812 kb on + strand, within CA265_RS19405 at 4817.847 kb on - strand, within CA265_RS19405 at 4817.877 kb on - strand, within CA265_RS19405 at 4817.914 kb on + strand, within CA265_RS19405 at 4817.930 kb on - strand, within CA265_RS19405 at 4818.037 kb on + strand, within CA265_RS19405 at 4818.037 kb on + strand, within CA265_RS19405 at 4818.038 kb on - strand, within CA265_RS19405 at 4818.058 kb on + strand, within CA265_RS19405 at 4818.104 kb on + strand, within CA265_RS19405 at 4818.138 kb on - strand, within CA265_RS19405 at 4818.138 kb on - strand, within CA265_RS19405 at 4818.159 kb on - strand, within CA265_RS19405 at 4818.235 kb on + strand at 4818.239 kb on + strand at 4818.240 kb on - strand at 4818.314 kb on + strand, within CA265_RS19410 at 4818.315 kb on - strand, within CA265_RS19410 at 4818.368 kb on + strand, within CA265_RS19410 at 4818.443 kb on + strand, within CA265_RS19410 at 4818.577 kb on - strand, within CA265_RS19410 at 4818.581 kb on - strand, within CA265_RS19410 at 4818.606 kb on + strand, within CA265_RS19410 at 4818.672 kb on - strand at 4818.696 kb on - strand at 4818.696 kb on - strand at 4818.733 kb on - strand at 4818.737 kb on - strand at 4818.857 kb on + strand, within CA265_RS19415 at 4818.857 kb on + strand, within CA265_RS19415 at 4818.858 kb on - strand, within CA265_RS19415 at 4818.858 kb on - strand, within CA265_RS19415 at 4818.858 kb on - strand, within CA265_RS19415 at 4818.945 kb on + strand, within CA265_RS19415 at 4819.001 kb on - strand, within CA265_RS19415 at 4819.043 kb on - strand at 4819.069 kb on - strand at 4819.075 kb on + strand at 4819.087 kb on + strand at 4819.087 kb on + strand at 4819.087 kb on + strand at 4819.087 kb on + strand at 4819.088 kb on - strand at 4819.119 kb on + strand at 4819.119 kb on + strand at 4819.119 kb on + strand at 4819.119 kb on + strand at 4819.273 kb on - strand, within CA265_RS19420 at 4819.371 kb on + strand at 4819.405 kb on + strand at 4819.490 kb on + strand at 4819.491 kb on - strand at 4819.492 kb on + strand at 4819.523 kb on - strand at 4819.629 kb on - strand, within CA265_RS19425 at 4819.683 kb on + strand, within CA265_RS19425 at 4819.684 kb on - strand, within CA265_RS19425 at 4819.761 kb on + strand, within CA265_RS19425 at 4819.761 kb on + strand, within CA265_RS19425 at 4819.803 kb on - strand, within CA265_RS19425 at 4819.877 kb on - strand, within CA265_RS19425 at 4819.885 kb on + strand, within CA265_RS19425 at 4819.930 kb on - strand, within CA265_RS19425 at 4819.956 kb on - strand, within CA265_RS19425 at 4819.956 kb on - strand, within CA265_RS19425 at 4819.962 kb on - strand, within CA265_RS19425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,817,812 + CA265_RS19405 0.17 -1.4 4,817,812 + CA265_RS19405 0.17 +0.8 4,817,812 + CA265_RS19405 0.17 -2.1 4,817,847 - CA265_RS19405 0.24 +0.8 4,817,877 - CA265_RS19405 0.30 +1.2 4,817,914 + CA265_RS19405 0.37 +0.1 4,817,930 - CA265_RS19405 0.40 -0.3 4,818,037 + CA265_RS19405 0.61 -0.1 4,818,037 + CA265_RS19405 0.61 -2.2 4,818,038 - CA265_RS19405 0.62 +0.2 4,818,058 + CA265_RS19405 0.65 -0.7 4,818,104 + CA265_RS19405 0.75 -0.4 4,818,138 - CA265_RS19405 0.81 +2.1 4,818,138 - CA265_RS19405 0.81 +0.7 4,818,159 - CA265_RS19405 0.85 -2.2 4,818,235 + +0.1 4,818,239 + +0.4 4,818,240 - +0.4 4,818,314 + CA265_RS19410 0.18 -0.7 4,818,315 - CA265_RS19410 0.18 -0.3 4,818,368 + CA265_RS19410 0.30 -2.0 4,818,443 + CA265_RS19410 0.46 +0.1 4,818,577 - CA265_RS19410 0.76 -0.0 4,818,581 - CA265_RS19410 0.77 -1.0 4,818,606 + CA265_RS19410 0.83 +0.4 4,818,672 - +1.0 4,818,696 - +0.3 4,818,696 - -0.1 4,818,733 - +0.4 4,818,737 - -0.3 4,818,857 + CA265_RS19415 0.29 -0.3 4,818,857 + CA265_RS19415 0.29 -1.7 4,818,858 - CA265_RS19415 0.30 -1.9 4,818,858 - CA265_RS19415 0.30 -3.3 4,818,858 - CA265_RS19415 0.30 -1.6 4,818,945 + CA265_RS19415 0.65 -2.6 4,819,001 - CA265_RS19415 0.87 -1.2 4,819,043 - +1.1 4,819,069 - -0.1 4,819,075 + -2.4 4,819,087 + -0.7 4,819,087 + -1.6 4,819,087 + -1.8 4,819,087 + -1.0 4,819,088 - +0.1 4,819,119 + +0.5 4,819,119 + -0.3 4,819,119 + +0.4 4,819,119 + +0.3 4,819,273 - CA265_RS19420 0.56 +0.0 4,819,371 + +0.6 4,819,405 + +0.6 4,819,490 + +1.0 4,819,491 - +1.2 4,819,492 + -1.9 4,819,523 - +1.5 4,819,629 - CA265_RS19425 0.16 +1.0 4,819,683 + CA265_RS19425 0.21 -0.4 4,819,684 - CA265_RS19425 0.21 -0.4 4,819,761 + CA265_RS19425 0.27 -0.2 4,819,761 + CA265_RS19425 0.27 +0.3 4,819,803 - CA265_RS19425 0.30 +0.4 4,819,877 - CA265_RS19425 0.36 -0.7 4,819,885 + CA265_RS19425 0.36 +0.3 4,819,930 - CA265_RS19425 0.40 +0.0 4,819,956 - CA265_RS19425 0.42 +0.3 4,819,956 - CA265_RS19425 0.42 +1.0 4,819,962 - CA265_RS19425 0.42 +0.4
Or see this region's nucleotide sequence