Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19310 and CA265_RS19315 are separated by 12 nucleotides CA265_RS19315 and CA265_RS19320 are separated by 169 nucleotides
CA265_RS19310: CA265_RS19310 - capsular biosynthesis protein, at 4,793,978 to 4,796,320
_RS19310
CA265_RS19315: CA265_RS19315 - hypothetical protein, at 4,796,333 to 4,797,139
_RS19315
CA265_RS19320: CA265_RS19320 - histidinol phosphatase, at 4,797,309 to 4,798,052
_RS19320
Position (kb)
4796
4797
4798 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4795.414 kb on + strand, within CA265_RS19310 at 4795.449 kb on - strand, within CA265_RS19310 at 4795.581 kb on - strand, within CA265_RS19310 at 4795.600 kb on + strand, within CA265_RS19310 at 4795.633 kb on + strand, within CA265_RS19310 at 4795.638 kb on - strand, within CA265_RS19310 at 4795.645 kb on + strand, within CA265_RS19310 at 4795.645 kb on + strand, within CA265_RS19310 at 4795.649 kb on - strand, within CA265_RS19310 at 4795.711 kb on + strand, within CA265_RS19310 at 4795.716 kb on + strand, within CA265_RS19310 at 4795.726 kb on + strand, within CA265_RS19310 at 4795.766 kb on + strand, within CA265_RS19310 at 4795.786 kb on + strand, within CA265_RS19310 at 4795.862 kb on + strand, within CA265_RS19310 at 4795.889 kb on - strand, within CA265_RS19310 at 4795.967 kb on + strand, within CA265_RS19310 at 4795.967 kb on + strand, within CA265_RS19310 at 4795.967 kb on + strand, within CA265_RS19310 at 4796.053 kb on + strand, within CA265_RS19310 at 4796.168 kb on - strand at 4796.169 kb on + strand at 4796.179 kb on - strand at 4796.181 kb on - strand at 4796.183 kb on - strand at 4796.238 kb on - strand at 4796.314 kb on + strand at 4796.315 kb on - strand at 4796.335 kb on - strand at 4796.435 kb on - strand, within CA265_RS19315 at 4796.448 kb on + strand, within CA265_RS19315 at 4796.452 kb on + strand, within CA265_RS19315 at 4796.506 kb on + strand, within CA265_RS19315 at 4796.642 kb on - strand, within CA265_RS19315 at 4796.683 kb on + strand, within CA265_RS19315 at 4796.705 kb on + strand, within CA265_RS19315 at 4796.706 kb on - strand, within CA265_RS19315 at 4796.706 kb on - strand, within CA265_RS19315 at 4796.763 kb on + strand, within CA265_RS19315 at 4796.767 kb on + strand, within CA265_RS19315 at 4796.855 kb on + strand, within CA265_RS19315 at 4796.869 kb on + strand, within CA265_RS19315 at 4796.884 kb on + strand, within CA265_RS19315 at 4796.947 kb on + strand, within CA265_RS19315 at 4797.016 kb on + strand, within CA265_RS19315 at 4797.016 kb on + strand, within CA265_RS19315 at 4797.052 kb on + strand, within CA265_RS19315 at 4797.124 kb on + strand at 4797.133 kb on + strand at 4797.145 kb on + strand at 4797.146 kb on - strand at 4797.146 kb on - strand at 4797.159 kb on + strand at 4797.167 kb on + strand at 4797.168 kb on - strand at 4797.168 kb on - strand at 4797.200 kb on - strand at 4797.213 kb on - strand at 4797.213 kb on - strand at 4797.242 kb on + strand at 4797.242 kb on + strand at 4797.243 kb on - strand at 4797.247 kb on + strand at 4797.247 kb on + strand at 4797.306 kb on + strand at 4797.348 kb on - strand at 4797.528 kb on - strand, within CA265_RS19320 at 4797.682 kb on + strand, within CA265_RS19320 at 4797.758 kb on + strand, within CA265_RS19320 at 4797.789 kb on + strand, within CA265_RS19320 at 4797.790 kb on - strand, within CA265_RS19320 at 4797.876 kb on - strand, within CA265_RS19320 at 4797.975 kb on + strand, within CA265_RS19320 at 4797.980 kb on + strand at 4798.052 kb on + strand at 4798.052 kb on + strand at 4798.053 kb on - strand at 4798.076 kb on - strand at 4798.078 kb on - strand at 4798.130 kb on + strand at 4798.135 kb on - strand at 4798.137 kb on - strand at 4798.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,795,414 + CA265_RS19310 0.61 -0.6 4,795,449 - CA265_RS19310 0.63 -1.0 4,795,581 - CA265_RS19310 0.68 -1.2 4,795,600 + CA265_RS19310 0.69 -1.9 4,795,633 + CA265_RS19310 0.71 -3.4 4,795,638 - CA265_RS19310 0.71 -1.0 4,795,645 + CA265_RS19310 0.71 -0.6 4,795,645 + CA265_RS19310 0.71 -0.8 4,795,649 - CA265_RS19310 0.71 -1.9 4,795,711 + CA265_RS19310 0.74 -2.2 4,795,716 + CA265_RS19310 0.74 -1.6 4,795,726 + CA265_RS19310 0.75 -1.6 4,795,766 + CA265_RS19310 0.76 -0.4 4,795,786 + CA265_RS19310 0.77 -3.5 4,795,862 + CA265_RS19310 0.80 -3.7 4,795,889 - CA265_RS19310 0.82 -1.1 4,795,967 + CA265_RS19310 0.85 -1.2 4,795,967 + CA265_RS19310 0.85 -1.2 4,795,967 + CA265_RS19310 0.85 -0.8 4,796,053 + CA265_RS19310 0.89 -2.4 4,796,168 - -1.0 4,796,169 + -2.7 4,796,179 - -1.0 4,796,181 - +0.2 4,796,183 - -0.8 4,796,238 - -0.8 4,796,314 + -1.3 4,796,315 - -1.8 4,796,335 - +0.1 4,796,435 - CA265_RS19315 0.13 +0.4 4,796,448 + CA265_RS19315 0.14 -0.6 4,796,452 + CA265_RS19315 0.15 -1.6 4,796,506 + CA265_RS19315 0.21 -3.3 4,796,642 - CA265_RS19315 0.38 +0.2 4,796,683 + CA265_RS19315 0.43 -2.9 4,796,705 + CA265_RS19315 0.46 -1.3 4,796,706 - CA265_RS19315 0.46 -1.3 4,796,706 - CA265_RS19315 0.46 -1.7 4,796,763 + CA265_RS19315 0.53 -0.0 4,796,767 + CA265_RS19315 0.54 -1.9 4,796,855 + CA265_RS19315 0.65 -0.7 4,796,869 + CA265_RS19315 0.66 +0.2 4,796,884 + CA265_RS19315 0.68 -3.2 4,796,947 + CA265_RS19315 0.76 -3.2 4,797,016 + CA265_RS19315 0.85 -2.5 4,797,016 + CA265_RS19315 0.85 -0.8 4,797,052 + CA265_RS19315 0.89 -1.5 4,797,124 + -1.3 4,797,133 + -3.4 4,797,145 + -2.2 4,797,146 - -1.6 4,797,146 - -0.3 4,797,159 + -1.9 4,797,167 + -0.7 4,797,168 - -0.4 4,797,168 - +0.6 4,797,200 - -0.2 4,797,213 - -0.5 4,797,213 - -0.7 4,797,242 + +0.3 4,797,242 + -0.6 4,797,243 - +0.4 4,797,247 + -0.0 4,797,247 + -0.9 4,797,306 + +0.1 4,797,348 - -0.8 4,797,528 - CA265_RS19320 0.29 +1.8 4,797,682 + CA265_RS19320 0.50 +0.2 4,797,758 + CA265_RS19320 0.60 +0.2 4,797,789 + CA265_RS19320 0.65 +0.2 4,797,790 - CA265_RS19320 0.65 -1.6 4,797,876 - CA265_RS19320 0.76 -1.4 4,797,975 + CA265_RS19320 0.90 -1.1 4,797,980 + -1.7 4,798,052 + -2.8 4,798,052 + -2.5 4,798,053 - +0.9 4,798,076 - +0.2 4,798,078 - +0.4 4,798,130 + -1.0 4,798,135 - +0.3 4,798,137 - +0.3 4,798,139 - +0.1
Or see this region's nucleotide sequence