Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19270 and CA265_RS19275 are separated by 41 nucleotides CA265_RS19275 and CA265_RS19280 overlap by 4 nucleotides
CA265_RS19270: CA265_RS19270 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 4,785,612 to 4,786,676
_RS19270
CA265_RS19275: CA265_RS19275 - hypothetical protein, at 4,786,718 to 4,787,344
_RS19275
CA265_RS19280: CA265_RS19280 - hypothetical protein, at 4,787,341 to 4,788,630
_RS19280
Position (kb)
4786
4787
4788 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4785.722 kb on + strand, within CA265_RS19270 at 4785.749 kb on + strand, within CA265_RS19270 at 4785.814 kb on - strand, within CA265_RS19270 at 4785.849 kb on - strand, within CA265_RS19270 at 4786.014 kb on + strand, within CA265_RS19270 at 4786.020 kb on + strand, within CA265_RS19270 at 4786.021 kb on - strand, within CA265_RS19270 at 4786.083 kb on - strand, within CA265_RS19270 at 4786.083 kb on - strand, within CA265_RS19270 at 4786.087 kb on - strand, within CA265_RS19270 at 4786.090 kb on - strand, within CA265_RS19270 at 4786.164 kb on + strand, within CA265_RS19270 at 4786.165 kb on - strand, within CA265_RS19270 at 4786.187 kb on + strand, within CA265_RS19270 at 4786.217 kb on + strand, within CA265_RS19270 at 4786.218 kb on - strand, within CA265_RS19270 at 4786.298 kb on + strand, within CA265_RS19270 at 4786.314 kb on + strand, within CA265_RS19270 at 4786.344 kb on - strand, within CA265_RS19270 at 4786.347 kb on + strand, within CA265_RS19270 at 4786.348 kb on - strand, within CA265_RS19270 at 4786.389 kb on - strand, within CA265_RS19270 at 4786.396 kb on + strand, within CA265_RS19270 at 4786.450 kb on - strand, within CA265_RS19270 at 4786.458 kb on - strand, within CA265_RS19270 at 4786.458 kb on - strand, within CA265_RS19270 at 4786.518 kb on - strand, within CA265_RS19270 at 4786.522 kb on - strand, within CA265_RS19270 at 4786.550 kb on + strand, within CA265_RS19270 at 4786.551 kb on - strand, within CA265_RS19270 at 4786.551 kb on - strand, within CA265_RS19270 at 4786.600 kb on - strand at 4786.611 kb on + strand at 4786.677 kb on - strand at 4786.726 kb on + strand at 4786.785 kb on + strand, within CA265_RS19275 at 4787.032 kb on + strand, within CA265_RS19275 at 4787.080 kb on + strand, within CA265_RS19275 at 4787.106 kb on + strand, within CA265_RS19275 at 4787.321 kb on + strand at 4787.322 kb on - strand at 4787.344 kb on + strand at 4787.346 kb on + strand at 4787.542 kb on + strand, within CA265_RS19280 at 4787.727 kb on + strand, within CA265_RS19280 at 4787.802 kb on + strand, within CA265_RS19280 at 4787.802 kb on + strand, within CA265_RS19280 at 4787.802 kb on + strand, within CA265_RS19280 at 4787.877 kb on + strand, within CA265_RS19280 at 4787.946 kb on + strand, within CA265_RS19280 at 4788.224 kb on - strand, within CA265_RS19280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,785,722 + CA265_RS19270 0.10 -1.6 4,785,749 + CA265_RS19270 0.13 -3.4 4,785,814 - CA265_RS19270 0.19 -2.4 4,785,849 - CA265_RS19270 0.22 -2.4 4,786,014 + CA265_RS19270 0.38 -1.3 4,786,020 + CA265_RS19270 0.38 -3.9 4,786,021 - CA265_RS19270 0.38 -2.1 4,786,083 - CA265_RS19270 0.44 -1.2 4,786,083 - CA265_RS19270 0.44 -0.6 4,786,087 - CA265_RS19270 0.45 -0.2 4,786,090 - CA265_RS19270 0.45 -0.4 4,786,164 + CA265_RS19270 0.52 -2.5 4,786,165 - CA265_RS19270 0.52 -1.1 4,786,187 + CA265_RS19270 0.54 -0.8 4,786,217 + CA265_RS19270 0.57 -3.3 4,786,218 - CA265_RS19270 0.57 -1.1 4,786,298 + CA265_RS19270 0.64 -3.6 4,786,314 + CA265_RS19270 0.66 -2.2 4,786,344 - CA265_RS19270 0.69 -1.4 4,786,347 + CA265_RS19270 0.69 -2.5 4,786,348 - CA265_RS19270 0.69 -1.1 4,786,389 - CA265_RS19270 0.73 -2.1 4,786,396 + CA265_RS19270 0.74 -2.9 4,786,450 - CA265_RS19270 0.79 -1.1 4,786,458 - CA265_RS19270 0.79 -0.2 4,786,458 - CA265_RS19270 0.79 -2.3 4,786,518 - CA265_RS19270 0.85 -1.6 4,786,522 - CA265_RS19270 0.85 +0.8 4,786,550 + CA265_RS19270 0.88 +0.3 4,786,551 - CA265_RS19270 0.88 -1.2 4,786,551 - CA265_RS19270 0.88 -2.3 4,786,600 - +1.3 4,786,611 + -2.5 4,786,677 - -2.2 4,786,726 + -1.0 4,786,785 + CA265_RS19275 0.11 +0.2 4,787,032 + CA265_RS19275 0.50 -1.8 4,787,080 + CA265_RS19275 0.58 -1.2 4,787,106 + CA265_RS19275 0.62 -0.2 4,787,321 + -1.4 4,787,322 - -0.2 4,787,344 + -3.4 4,787,346 + -0.1 4,787,542 + CA265_RS19280 0.16 -0.6 4,787,727 + CA265_RS19280 0.30 -1.2 4,787,802 + CA265_RS19280 0.36 -1.7 4,787,802 + CA265_RS19280 0.36 -1.4 4,787,802 + CA265_RS19280 0.36 -2.4 4,787,877 + CA265_RS19280 0.42 -1.7 4,787,946 + CA265_RS19280 0.47 +1.0 4,788,224 - CA265_RS19280 0.68 -1.0
Or see this region's nucleotide sequence