Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19255 and CA265_RS19260 are separated by 3 nucleotides CA265_RS19260 and CA265_RS19265 are separated by 0 nucleotides CA265_RS19265 and CA265_RS19270 are separated by 24 nucleotides
CA265_RS19255: CA265_RS19255 - ExsB family protein, at 4,783,068 to 4,784,192
_RS19255
CA265_RS19260: CA265_RS19260 - imidazole glycerol phosphate synthase subunit HisF, at 4,784,196 to 4,784,963
_RS19260
CA265_RS19265: CA265_RS19265 - imidazole glycerol phosphate synthase subunit HisH, at 4,784,964 to 4,785,587
_RS19265
CA265_RS19270: CA265_RS19270 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 4,785,612 to 4,786,676
_RS19270
Position (kb)
4784
4785 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4783.397 kb on - strand, within CA265_RS19255 at 4783.423 kb on + strand, within CA265_RS19255 at 4783.484 kb on + strand, within CA265_RS19255 at 4783.516 kb on + strand, within CA265_RS19255 at 4783.516 kb on + strand, within CA265_RS19255 at 4783.658 kb on + strand, within CA265_RS19255 at 4783.821 kb on - strand, within CA265_RS19255 at 4783.948 kb on + strand, within CA265_RS19255 at 4783.956 kb on - strand, within CA265_RS19255 at 4784.112 kb on + strand at 4784.112 kb on + strand at 4784.130 kb on + strand at 4784.172 kb on + strand at 4784.221 kb on - strand at 4784.230 kb on + strand at 4784.240 kb on - strand at 4784.240 kb on - strand at 4784.398 kb on - strand, within CA265_RS19260 at 4784.421 kb on + strand, within CA265_RS19260 at 4784.427 kb on - strand, within CA265_RS19260 at 4784.427 kb on - strand, within CA265_RS19260 at 4784.544 kb on - strand, within CA265_RS19260 at 4784.575 kb on - strand, within CA265_RS19260 at 4784.640 kb on + strand, within CA265_RS19260 at 4784.712 kb on - strand, within CA265_RS19260 at 4784.764 kb on - strand, within CA265_RS19260 at 4784.765 kb on + strand, within CA265_RS19260 at 4784.766 kb on - strand, within CA265_RS19260 at 4784.879 kb on - strand, within CA265_RS19260 at 4784.945 kb on + strand at 4784.968 kb on - strand at 4785.150 kb on + strand, within CA265_RS19265 at 4785.175 kb on - strand, within CA265_RS19265 at 4785.188 kb on + strand, within CA265_RS19265 at 4785.189 kb on - strand, within CA265_RS19265 at 4785.373 kb on - strand, within CA265_RS19265 at 4785.450 kb on + strand, within CA265_RS19265 at 4785.524 kb on + strand, within CA265_RS19265 at 4785.568 kb on + strand at 4785.571 kb on - strand at 4785.613 kb on + strand at 4785.684 kb on + strand at 4785.722 kb on + strand, within CA265_RS19270 at 4785.749 kb on + strand, within CA265_RS19270 at 4785.814 kb on - strand, within CA265_RS19270 at 4785.849 kb on - strand, within CA265_RS19270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,783,397 - CA265_RS19255 0.29 -0.2 4,783,423 + CA265_RS19255 0.32 -0.5 4,783,484 + CA265_RS19255 0.37 -1.2 4,783,516 + CA265_RS19255 0.40 -1.4 4,783,516 + CA265_RS19255 0.40 -2.1 4,783,658 + CA265_RS19255 0.52 -2.0 4,783,821 - CA265_RS19255 0.67 -1.2 4,783,948 + CA265_RS19255 0.78 -0.8 4,783,956 - CA265_RS19255 0.79 -1.6 4,784,112 + -0.2 4,784,112 + +0.8 4,784,130 + +0.2 4,784,172 + -2.2 4,784,221 - -0.6 4,784,230 + -1.2 4,784,240 - -0.2 4,784,240 - +0.8 4,784,398 - CA265_RS19260 0.26 -4.2 4,784,421 + CA265_RS19260 0.29 -1.2 4,784,427 - CA265_RS19260 0.30 -2.3 4,784,427 - CA265_RS19260 0.30 -0.7 4,784,544 - CA265_RS19260 0.45 -0.3 4,784,575 - CA265_RS19260 0.49 -1.2 4,784,640 + CA265_RS19260 0.58 -1.4 4,784,712 - CA265_RS19260 0.67 -1.7 4,784,764 - CA265_RS19260 0.74 -0.6 4,784,765 + CA265_RS19260 0.74 -1.2 4,784,766 - CA265_RS19260 0.74 -1.9 4,784,879 - CA265_RS19260 0.89 -2.4 4,784,945 + -1.2 4,784,968 - -2.2 4,785,150 + CA265_RS19265 0.30 -1.1 4,785,175 - CA265_RS19265 0.34 -1.9 4,785,188 + CA265_RS19265 0.36 -0.2 4,785,189 - CA265_RS19265 0.36 -1.4 4,785,373 - CA265_RS19265 0.66 -0.6 4,785,450 + CA265_RS19265 0.78 -2.7 4,785,524 + CA265_RS19265 0.90 -1.7 4,785,568 + -2.2 4,785,571 - -2.0 4,785,613 + -1.6 4,785,684 + -1.6 4,785,722 + CA265_RS19270 0.10 -1.6 4,785,749 + CA265_RS19270 0.13 -3.4 4,785,814 - CA265_RS19270 0.19 -2.4 4,785,849 - CA265_RS19270 0.22 -2.4
Or see this region's nucleotide sequence