Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19230 and CA265_RS19235 overlap by 7 nucleotides CA265_RS19235 and CA265_RS19240 are separated by 4 nucleotides CA265_RS19240 and CA265_RS19245 are separated by 134 nucleotides
CA265_RS19230: CA265_RS19230 - hypothetical protein, at 4,778,859 to 4,779,971
_RS19230
CA265_RS19235: CA265_RS19235 - hypothetical protein, at 4,779,965 to 4,780,840
_RS19235
CA265_RS19240: CA265_RS19240 - hypothetical protein, at 4,780,845 to 4,781,102
_RS19240
CA265_RS19245: CA265_RS19245 - hypothetical protein, at 4,781,237 to 4,781,827
_RS19245
Position (kb)
4779
4780
4781 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4779.012 kb on - strand, within CA265_RS19230 at 4779.012 kb on - strand, within CA265_RS19230 at 4779.048 kb on + strand, within CA265_RS19230 at 4779.051 kb on + strand, within CA265_RS19230 at 4779.051 kb on + strand, within CA265_RS19230 at 4779.057 kb on - strand, within CA265_RS19230 at 4779.111 kb on + strand, within CA265_RS19230 at 4779.201 kb on + strand, within CA265_RS19230 at 4779.273 kb on - strand, within CA265_RS19230 at 4779.353 kb on + strand, within CA265_RS19230 at 4779.395 kb on - strand, within CA265_RS19230 at 4779.399 kb on + strand, within CA265_RS19230 at 4779.399 kb on + strand, within CA265_RS19230 at 4779.443 kb on - strand, within CA265_RS19230 at 4779.470 kb on + strand, within CA265_RS19230 at 4779.471 kb on - strand, within CA265_RS19230 at 4779.474 kb on + strand, within CA265_RS19230 at 4779.530 kb on + strand, within CA265_RS19230 at 4779.569 kb on + strand, within CA265_RS19230 at 4779.727 kb on + strand, within CA265_RS19230 at 4779.743 kb on + strand, within CA265_RS19230 at 4779.758 kb on - strand, within CA265_RS19230 at 4779.771 kb on + strand, within CA265_RS19230 at 4779.773 kb on + strand, within CA265_RS19230 at 4779.773 kb on + strand, within CA265_RS19230 at 4779.773 kb on + strand, within CA265_RS19230 at 4779.805 kb on - strand, within CA265_RS19230 at 4779.852 kb on + strand, within CA265_RS19230 at 4779.853 kb on - strand, within CA265_RS19230 at 4779.861 kb on + strand at 4779.971 kb on + strand at 4779.971 kb on + strand at 4779.972 kb on - strand at 4779.984 kb on - strand at 4780.036 kb on + strand at 4780.036 kb on + strand at 4780.036 kb on + strand at 4780.037 kb on - strand at 4780.086 kb on + strand, within CA265_RS19235 at 4780.335 kb on - strand, within CA265_RS19235 at 4780.345 kb on + strand, within CA265_RS19235 at 4780.798 kb on + strand at 4780.999 kb on + strand, within CA265_RS19240 at 4780.999 kb on + strand, within CA265_RS19240 at 4781.109 kb on + strand at 4781.125 kb on + strand at 4781.164 kb on + strand at 4781.186 kb on - strand at 4781.196 kb on + strand at 4781.223 kb on + strand at 4781.242 kb on + strand at 4781.242 kb on + strand at 4781.274 kb on + strand at 4781.274 kb on + strand at 4781.274 kb on + strand at 4781.274 kb on + strand at 4781.275 kb on - strand at 4781.276 kb on + strand at 4781.276 kb on + strand at 4781.276 kb on + strand at 4781.277 kb on - strand at 4781.277 kb on - strand at 4781.311 kb on + strand, within CA265_RS19245 at 4781.324 kb on + strand, within CA265_RS19245 at 4781.325 kb on - strand, within CA265_RS19245 at 4781.325 kb on - strand, within CA265_RS19245 at 4781.393 kb on + strand, within CA265_RS19245 at 4781.426 kb on - strand, within CA265_RS19245 at 4781.495 kb on + strand, within CA265_RS19245 at 4781.496 kb on - strand, within CA265_RS19245 at 4781.496 kb on - strand, within CA265_RS19245 at 4781.533 kb on + strand, within CA265_RS19245 at 4781.533 kb on + strand, within CA265_RS19245 at 4781.534 kb on - strand, within CA265_RS19245 at 4781.537 kb on + strand, within CA265_RS19245 at 4781.538 kb on - strand, within CA265_RS19245 at 4781.538 kb on - strand, within CA265_RS19245 at 4781.550 kb on - strand, within CA265_RS19245 at 4781.607 kb on - strand, within CA265_RS19245 at 4781.619 kb on - strand, within CA265_RS19245 at 4781.628 kb on - strand, within CA265_RS19245 at 4781.638 kb on + strand, within CA265_RS19245 at 4781.639 kb on - strand, within CA265_RS19245 at 4781.643 kb on + strand, within CA265_RS19245 at 4781.649 kb on + strand, within CA265_RS19245 at 4781.649 kb on + strand, within CA265_RS19245 at 4781.649 kb on + strand, within CA265_RS19245 at 4781.650 kb on - strand, within CA265_RS19245 at 4781.650 kb on - strand, within CA265_RS19245 at 4781.693 kb on + strand, within CA265_RS19245 at 4781.693 kb on + strand, within CA265_RS19245 at 4781.694 kb on - strand, within CA265_RS19245 at 4781.696 kb on - strand, within CA265_RS19245 at 4781.721 kb on + strand, within CA265_RS19245 at 4781.725 kb on + strand, within CA265_RS19245 at 4781.819 kb on - strand at 4781.827 kb on + strand at 4781.828 kb on - strand at 4781.836 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,779,012 - CA265_RS19230 0.14 -0.8 4,779,012 - CA265_RS19230 0.14 -1.4 4,779,048 + CA265_RS19230 0.17 -2.2 4,779,051 + CA265_RS19230 0.17 -0.4 4,779,051 + CA265_RS19230 0.17 -3.1 4,779,057 - CA265_RS19230 0.18 -1.2 4,779,111 + CA265_RS19230 0.23 -1.7 4,779,201 + CA265_RS19230 0.31 -0.7 4,779,273 - CA265_RS19230 0.37 -1.4 4,779,353 + CA265_RS19230 0.44 -1.9 4,779,395 - CA265_RS19230 0.48 -2.8 4,779,399 + CA265_RS19230 0.49 -2.1 4,779,399 + CA265_RS19230 0.49 +0.2 4,779,443 - CA265_RS19230 0.52 -1.6 4,779,470 + CA265_RS19230 0.55 -2.0 4,779,471 - CA265_RS19230 0.55 -2.5 4,779,474 + CA265_RS19230 0.55 -2.5 4,779,530 + CA265_RS19230 0.60 -1.9 4,779,569 + CA265_RS19230 0.64 -3.3 4,779,727 + CA265_RS19230 0.78 -1.9 4,779,743 + CA265_RS19230 0.79 -1.9 4,779,758 - CA265_RS19230 0.81 -3.0 4,779,771 + CA265_RS19230 0.82 +0.4 4,779,773 + CA265_RS19230 0.82 -1.5 4,779,773 + CA265_RS19230 0.82 -2.6 4,779,773 + CA265_RS19230 0.82 -0.9 4,779,805 - CA265_RS19230 0.85 -1.1 4,779,852 + CA265_RS19230 0.89 -2.3 4,779,853 - CA265_RS19230 0.89 -3.5 4,779,861 + -2.5 4,779,971 + -3.3 4,779,971 + -3.6 4,779,972 - -2.5 4,779,984 - -0.4 4,780,036 + -2.5 4,780,036 + -2.1 4,780,036 + -1.0 4,780,037 - -0.6 4,780,086 + CA265_RS19235 0.14 -2.1 4,780,335 - CA265_RS19235 0.42 -1.2 4,780,345 + CA265_RS19235 0.43 -3.1 4,780,798 + -1.1 4,780,999 + CA265_RS19240 0.60 -3.7 4,780,999 + CA265_RS19240 0.60 -2.6 4,781,109 + -1.7 4,781,125 + -1.5 4,781,164 + +1.1 4,781,186 - -2.7 4,781,196 + -0.6 4,781,223 + -0.8 4,781,242 + +0.2 4,781,242 + -0.9 4,781,274 + -2.2 4,781,274 + -0.0 4,781,274 + -1.4 4,781,274 + -0.3 4,781,275 - -0.2 4,781,276 + -0.8 4,781,276 + -0.7 4,781,276 + -0.1 4,781,277 - -2.2 4,781,277 - -2.7 4,781,311 + CA265_RS19245 0.13 -1.4 4,781,324 + CA265_RS19245 0.15 -1.5 4,781,325 - CA265_RS19245 0.15 -2.3 4,781,325 - CA265_RS19245 0.15 -3.6 4,781,393 + CA265_RS19245 0.26 -0.7 4,781,426 - CA265_RS19245 0.32 -1.3 4,781,495 + CA265_RS19245 0.44 -0.3 4,781,496 - CA265_RS19245 0.44 -2.8 4,781,496 - CA265_RS19245 0.44 -3.3 4,781,533 + CA265_RS19245 0.50 -1.3 4,781,533 + CA265_RS19245 0.50 +1.8 4,781,534 - CA265_RS19245 0.50 -1.9 4,781,537 + CA265_RS19245 0.51 +0.3 4,781,538 - CA265_RS19245 0.51 -0.4 4,781,538 - CA265_RS19245 0.51 -1.4 4,781,550 - CA265_RS19245 0.53 -3.3 4,781,607 - CA265_RS19245 0.63 -1.8 4,781,619 - CA265_RS19245 0.65 -2.2 4,781,628 - CA265_RS19245 0.66 -2.2 4,781,638 + CA265_RS19245 0.68 -1.4 4,781,639 - CA265_RS19245 0.68 -3.4 4,781,643 + CA265_RS19245 0.69 -0.3 4,781,649 + CA265_RS19245 0.70 -0.2 4,781,649 + CA265_RS19245 0.70 -0.2 4,781,649 + CA265_RS19245 0.70 -1.6 4,781,650 - CA265_RS19245 0.70 -0.6 4,781,650 - CA265_RS19245 0.70 +0.8 4,781,693 + CA265_RS19245 0.77 -1.4 4,781,693 + CA265_RS19245 0.77 -1.2 4,781,694 - CA265_RS19245 0.77 -2.6 4,781,696 - CA265_RS19245 0.78 -1.9 4,781,721 + CA265_RS19245 0.82 -0.5 4,781,725 + CA265_RS19245 0.83 -1.6 4,781,819 - -2.6 4,781,827 + -1.0 4,781,828 - -2.2 4,781,836 - -1.1
Or see this region's nucleotide sequence