Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19185 and CA265_RS19190 are separated by 45 nucleotides CA265_RS19190 and CA265_RS19195 overlap by 8 nucleotides CA265_RS19195 and CA265_RS19200 are separated by 103 nucleotides
CA265_RS19185: CA265_RS19185 - polysaccharide biosynthesis protein, at 4,769,324 to 4,771,264
_RS19185
CA265_RS19190: CA265_RS19190 - transferase, at 4,771,310 to 4,771,858
_RS19190
CA265_RS19195: CA265_RS19195 - UDP-GlcNAc--UDP-phosphate GlcNAc-1-phosphate transferase, at 4,771,851 to 4,772,831
_RS19195
CA265_RS19200: CA265_RS19200 - mannose-1-phosphate guanylyltransferase, at 4,772,935 to 4,773,984
_RS19200
Position (kb)
4771
4772
4773 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4770.855 kb on + strand, within CA265_RS19185 at 4770.871 kb on + strand, within CA265_RS19185 at 4770.886 kb on - strand, within CA265_RS19185 at 4771.009 kb on + strand, within CA265_RS19185 at 4771.019 kb on + strand, within CA265_RS19185 at 4771.045 kb on + strand, within CA265_RS19185 at 4771.045 kb on + strand, within CA265_RS19185 at 4771.045 kb on + strand, within CA265_RS19185 at 4771.046 kb on - strand, within CA265_RS19185 at 4771.060 kb on + strand, within CA265_RS19185 at 4771.067 kb on - strand, within CA265_RS19185 at 4771.068 kb on + strand, within CA265_RS19185 at 4771.068 kb on + strand, within CA265_RS19185 at 4771.069 kb on - strand, within CA265_RS19185 at 4771.075 kb on + strand at 4771.078 kb on + strand at 4771.079 kb on - strand at 4771.099 kb on + strand at 4771.099 kb on + strand at 4771.100 kb on - strand at 4771.154 kb on + strand at 4771.166 kb on - strand at 4771.166 kb on - strand at 4771.267 kb on + strand at 4771.273 kb on - strand at 4771.293 kb on + strand at 4771.294 kb on - strand at 4771.300 kb on + strand at 4771.300 kb on + strand at 4771.301 kb on - strand at 4771.314 kb on + strand at 4771.314 kb on + strand at 4771.401 kb on - strand, within CA265_RS19190 at 4771.435 kb on - strand, within CA265_RS19190 at 4771.448 kb on + strand, within CA265_RS19190 at 4771.448 kb on + strand, within CA265_RS19190 at 4771.448 kb on + strand, within CA265_RS19190 at 4771.449 kb on - strand, within CA265_RS19190 at 4771.449 kb on - strand, within CA265_RS19190 at 4771.450 kb on + strand, within CA265_RS19190 at 4771.491 kb on - strand, within CA265_RS19190 at 4771.598 kb on - strand, within CA265_RS19190 at 4771.598 kb on - strand, within CA265_RS19190 at 4771.631 kb on + strand, within CA265_RS19190 at 4771.631 kb on + strand, within CA265_RS19190 at 4771.632 kb on - strand, within CA265_RS19190 at 4771.636 kb on + strand, within CA265_RS19190 at 4771.637 kb on - strand, within CA265_RS19190 at 4771.659 kb on - strand, within CA265_RS19190 at 4771.669 kb on + strand, within CA265_RS19190 at 4771.756 kb on + strand, within CA265_RS19190 at 4771.757 kb on - strand, within CA265_RS19190 at 4771.863 kb on + strand at 4771.864 kb on - strand at 4772.116 kb on - strand, within CA265_RS19195 at 4772.152 kb on + strand, within CA265_RS19195 at 4772.152 kb on + strand, within CA265_RS19195 at 4772.172 kb on - strand, within CA265_RS19195 at 4772.217 kb on + strand, within CA265_RS19195 at 4772.239 kb on - strand, within CA265_RS19195 at 4772.248 kb on - strand, within CA265_RS19195 at 4772.311 kb on - strand, within CA265_RS19195 at 4772.342 kb on + strand, within CA265_RS19195 at 4772.378 kb on + strand, within CA265_RS19195 at 4772.379 kb on - strand, within CA265_RS19195 at 4772.408 kb on + strand, within CA265_RS19195 at 4772.517 kb on - strand, within CA265_RS19195 at 4772.521 kb on - strand, within CA265_RS19195 at 4772.521 kb on - strand, within CA265_RS19195 at 4772.550 kb on + strand, within CA265_RS19195 at 4772.580 kb on - strand, within CA265_RS19195 at 4772.748 kb on - strand at 4772.811 kb on + strand at 4772.820 kb on + strand at 4772.822 kb on + strand at 4772.912 kb on + strand at 4772.920 kb on + strand at 4772.938 kb on + strand at 4772.938 kb on + strand at 4772.962 kb on + strand at 4773.042 kb on + strand, within CA265_RS19200 at 4773.042 kb on + strand, within CA265_RS19200 at 4773.043 kb on - strand, within CA265_RS19200 at 4773.047 kb on + strand, within CA265_RS19200 at 4773.063 kb on + strand, within CA265_RS19200 at 4773.081 kb on + strand, within CA265_RS19200 at 4773.094 kb on + strand, within CA265_RS19200 at 4773.095 kb on - strand, within CA265_RS19200 at 4773.099 kb on + strand, within CA265_RS19200 at 4773.105 kb on + strand, within CA265_RS19200 at 4773.125 kb on - strand, within CA265_RS19200 at 4773.178 kb on + strand, within CA265_RS19200 at 4773.179 kb on - strand, within CA265_RS19200 at 4773.179 kb on - strand, within CA265_RS19200 at 4773.228 kb on + strand, within CA265_RS19200 at 4773.228 kb on + strand, within CA265_RS19200 at 4773.233 kb on - strand, within CA265_RS19200 at 4773.233 kb on - strand, within CA265_RS19200 at 4773.254 kb on - strand, within CA265_RS19200 at 4773.254 kb on - strand, within CA265_RS19200 at 4773.254 kb on - strand, within CA265_RS19200 at 4773.290 kb on + strand, within CA265_RS19200 at 4773.305 kb on + strand, within CA265_RS19200 at 4773.330 kb on - strand, within CA265_RS19200 at 4773.330 kb on - strand, within CA265_RS19200 at 4773.362 kb on - strand, within CA265_RS19200 at 4773.381 kb on + strand, within CA265_RS19200 at 4773.382 kb on - strand, within CA265_RS19200 at 4773.534 kb on + strand, within CA265_RS19200 at 4773.534 kb on + strand, within CA265_RS19200 at 4773.534 kb on + strand, within CA265_RS19200 at 4773.534 kb on + strand, within CA265_RS19200 at 4773.535 kb on - strand, within CA265_RS19200 at 4773.538 kb on - strand, within CA265_RS19200 at 4773.540 kb on - strand, within CA265_RS19200 at 4773.571 kb on - strand, within CA265_RS19200 at 4773.582 kb on + strand, within CA265_RS19200 at 4773.582 kb on + strand, within CA265_RS19200 at 4773.631 kb on - strand, within CA265_RS19200 at 4773.631 kb on - strand, within CA265_RS19200 at 4773.657 kb on + strand, within CA265_RS19200 at 4773.684 kb on + strand, within CA265_RS19200 at 4773.684 kb on + strand, within CA265_RS19200 at 4773.745 kb on - strand, within CA265_RS19200 at 4773.769 kb on + strand, within CA265_RS19200 at 4773.770 kb on - strand, within CA265_RS19200 at 4773.775 kb on - strand, within CA265_RS19200 at 4773.775 kb on - strand, within CA265_RS19200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,770,855 + CA265_RS19185 0.79 -1.8 4,770,871 + CA265_RS19185 0.80 -2.1 4,770,886 - CA265_RS19185 0.80 -1.9 4,771,009 + CA265_RS19185 0.87 -0.7 4,771,019 + CA265_RS19185 0.87 -2.2 4,771,045 + CA265_RS19185 0.89 -3.4 4,771,045 + CA265_RS19185 0.89 -0.8 4,771,045 + CA265_RS19185 0.89 +0.2 4,771,046 - CA265_RS19185 0.89 -1.8 4,771,060 + CA265_RS19185 0.89 -1.3 4,771,067 - CA265_RS19185 0.90 -1.4 4,771,068 + CA265_RS19185 0.90 -2.6 4,771,068 + CA265_RS19185 0.90 -1.8 4,771,069 - CA265_RS19185 0.90 -1.8 4,771,075 + -3.0 4,771,078 + -0.8 4,771,079 - -2.4 4,771,099 + -1.1 4,771,099 + -1.5 4,771,100 - -2.4 4,771,154 + -0.7 4,771,166 - -3.5 4,771,166 - -3.3 4,771,267 + -0.3 4,771,273 - -0.7 4,771,293 + -1.5 4,771,294 - +0.2 4,771,300 + -2.1 4,771,300 + -3.4 4,771,301 - +0.7 4,771,314 + -1.6 4,771,314 + -1.5 4,771,401 - CA265_RS19190 0.17 +0.4 4,771,435 - CA265_RS19190 0.23 -0.6 4,771,448 + CA265_RS19190 0.25 -1.3 4,771,448 + CA265_RS19190 0.25 -0.6 4,771,448 + CA265_RS19190 0.25 -1.1 4,771,449 - CA265_RS19190 0.25 +0.9 4,771,449 - CA265_RS19190 0.25 +0.4 4,771,450 + CA265_RS19190 0.26 -1.6 4,771,491 - CA265_RS19190 0.33 -0.5 4,771,598 - CA265_RS19190 0.52 -1.1 4,771,598 - CA265_RS19190 0.52 -0.1 4,771,631 + CA265_RS19190 0.58 +2.7 4,771,631 + CA265_RS19190 0.58 -0.6 4,771,632 - CA265_RS19190 0.59 +0.4 4,771,636 + CA265_RS19190 0.59 -0.1 4,771,637 - CA265_RS19190 0.60 +0.5 4,771,659 - CA265_RS19190 0.64 -0.2 4,771,669 + CA265_RS19190 0.65 +0.6 4,771,756 + CA265_RS19190 0.81 -0.3 4,771,757 - CA265_RS19190 0.81 -0.4 4,771,863 + -0.8 4,771,864 - -0.8 4,772,116 - CA265_RS19195 0.27 -2.2 4,772,152 + CA265_RS19195 0.31 -3.3 4,772,152 + CA265_RS19195 0.31 -2.6 4,772,172 - CA265_RS19195 0.33 -0.7 4,772,217 + CA265_RS19195 0.37 -0.8 4,772,239 - CA265_RS19195 0.40 -3.8 4,772,248 - CA265_RS19195 0.40 -2.6 4,772,311 - CA265_RS19195 0.47 -2.3 4,772,342 + CA265_RS19195 0.50 -1.3 4,772,378 + CA265_RS19195 0.54 -1.7 4,772,379 - CA265_RS19195 0.54 -0.1 4,772,408 + CA265_RS19195 0.57 -1.3 4,772,517 - CA265_RS19195 0.68 -1.7 4,772,521 - CA265_RS19195 0.68 -1.8 4,772,521 - CA265_RS19195 0.68 -0.8 4,772,550 + CA265_RS19195 0.71 -0.3 4,772,580 - CA265_RS19195 0.74 -1.7 4,772,748 - -0.8 4,772,811 + -0.1 4,772,820 + -1.1 4,772,822 + -2.7 4,772,912 + +0.4 4,772,920 + +0.8 4,772,938 + +1.0 4,772,938 + -0.2 4,772,962 + +1.9 4,773,042 + CA265_RS19200 0.10 -0.1 4,773,042 + CA265_RS19200 0.10 -0.5 4,773,043 - CA265_RS19200 0.10 +0.4 4,773,047 + CA265_RS19200 0.11 -0.3 4,773,063 + CA265_RS19200 0.12 +0.3 4,773,081 + CA265_RS19200 0.14 -0.4 4,773,094 + CA265_RS19200 0.15 -0.8 4,773,095 - CA265_RS19200 0.15 +0.9 4,773,099 + CA265_RS19200 0.16 -0.2 4,773,105 + CA265_RS19200 0.16 +0.8 4,773,125 - CA265_RS19200 0.18 +0.3 4,773,178 + CA265_RS19200 0.23 +0.1 4,773,179 - CA265_RS19200 0.23 +1.3 4,773,179 - CA265_RS19200 0.23 -0.3 4,773,228 + CA265_RS19200 0.28 -0.1 4,773,228 + CA265_RS19200 0.28 -0.6 4,773,233 - CA265_RS19200 0.28 -0.2 4,773,233 - CA265_RS19200 0.28 +0.1 4,773,254 - CA265_RS19200 0.30 -0.2 4,773,254 - CA265_RS19200 0.30 -0.9 4,773,254 - CA265_RS19200 0.30 -0.7 4,773,290 + CA265_RS19200 0.34 -1.0 4,773,305 + CA265_RS19200 0.35 -0.0 4,773,330 - CA265_RS19200 0.38 -0.4 4,773,330 - CA265_RS19200 0.38 -0.1 4,773,362 - CA265_RS19200 0.41 +0.6 4,773,381 + CA265_RS19200 0.42 +0.0 4,773,382 - CA265_RS19200 0.43 -1.6 4,773,534 + CA265_RS19200 0.57 +0.2 4,773,534 + CA265_RS19200 0.57 -0.2 4,773,534 + CA265_RS19200 0.57 -2.6 4,773,534 + CA265_RS19200 0.57 -0.9 4,773,535 - CA265_RS19200 0.57 -0.5 4,773,538 - CA265_RS19200 0.57 -0.9 4,773,540 - CA265_RS19200 0.58 +0.2 4,773,571 - CA265_RS19200 0.61 -0.0 4,773,582 + CA265_RS19200 0.62 -0.8 4,773,582 + CA265_RS19200 0.62 +0.3 4,773,631 - CA265_RS19200 0.66 -1.4 4,773,631 - CA265_RS19200 0.66 +1.0 4,773,657 + CA265_RS19200 0.69 -0.2 4,773,684 + CA265_RS19200 0.71 -0.0 4,773,684 + CA265_RS19200 0.71 -0.7 4,773,745 - CA265_RS19200 0.77 -0.6 4,773,769 + CA265_RS19200 0.79 +0.1 4,773,770 - CA265_RS19200 0.80 +2.5 4,773,775 - CA265_RS19200 0.80 -0.2 4,773,775 - CA265_RS19200 0.80 -0.2
Or see this region's nucleotide sequence