Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19010 and CA265_RS19015 are separated by 18 nucleotides CA265_RS19015 and CA265_RS19020 are separated by 34 nucleotides CA265_RS19020 and CA265_RS19025 are separated by 47 nucleotides CA265_RS19025 and CA265_RS19030 are separated by 185 nucleotides CA265_RS19030 and CA265_RS19035 overlap by 23 nucleotides
CA265_RS19010: CA265_RS19010 - aspartyl/glutamyl-tRNA amidotransferase subunit A, at 4,731,083 to 4,732,501
_RS19010
CA265_RS19015: CA265_RS19015 - twin-arginine translocase TatA/TatE family subunit, at 4,732,520 to 4,732,696
_RS19015
CA265_RS19020: CA265_RS19020 - twin-arginine translocase TatA/TatE family subunit, at 4,732,731 to 4,733,090
_RS19020
CA265_RS19025: CA265_RS19025 - peptidase M23, at 4,733,138 to 4,734,433
_RS19025
CA265_RS19030: CA265_RS19030 - hypothetical protein, at 4,734,619 to 4,735,410
_RS19030
CA265_RS19035: CA265_RS19035 - hypothetical protein, at 4,735,388 to 4,737,085
_RS19035
Position (kb)
4733
4734
4735 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4733.201 kb on + strand at 4733.264 kb on + strand at 4733.265 kb on - strand at 4733.296 kb on - strand, within CA265_RS19025 at 4733.296 kb on - strand, within CA265_RS19025 at 4733.349 kb on - strand, within CA265_RS19025 at 4733.369 kb on - strand, within CA265_RS19025 at 4733.411 kb on - strand, within CA265_RS19025 at 4733.411 kb on - strand, within CA265_RS19025 at 4733.453 kb on - strand, within CA265_RS19025 at 4733.501 kb on + strand, within CA265_RS19025 at 4733.502 kb on - strand, within CA265_RS19025 at 4733.561 kb on - strand, within CA265_RS19025 at 4733.600 kb on - strand, within CA265_RS19025 at 4733.612 kb on - strand, within CA265_RS19025 at 4733.612 kb on - strand, within CA265_RS19025 at 4733.616 kb on - strand, within CA265_RS19025 at 4733.779 kb on + strand, within CA265_RS19025 at 4733.846 kb on - strand, within CA265_RS19025 at 4733.851 kb on + strand, within CA265_RS19025 at 4733.906 kb on - strand, within CA265_RS19025 at 4733.914 kb on + strand, within CA265_RS19025 at 4733.953 kb on + strand, within CA265_RS19025 at 4733.964 kb on + strand, within CA265_RS19025 at 4733.992 kb on + strand, within CA265_RS19025 at 4734.003 kb on - strand, within CA265_RS19025 at 4734.112 kb on - strand, within CA265_RS19025 at 4734.153 kb on - strand, within CA265_RS19025 at 4734.153 kb on - strand, within CA265_RS19025 at 4734.266 kb on + strand, within CA265_RS19025 at 4734.269 kb on - strand, within CA265_RS19025 at 4734.269 kb on - strand, within CA265_RS19025 at 4734.332 kb on + strand at 4734.333 kb on - strand at 4734.335 kb on - strand at 4734.559 kb on + strand at 4734.563 kb on + strand at 4734.564 kb on - strand at 4734.565 kb on + strand at 4734.565 kb on + strand at 4734.565 kb on + strand at 4734.566 kb on - strand at 4734.570 kb on + strand at 4734.570 kb on + strand at 4734.574 kb on + strand at 4734.605 kb on + strand at 4734.625 kb on + strand at 4734.652 kb on - strand at 4734.697 kb on + strand at 4734.751 kb on - strand, within CA265_RS19030 at 4734.757 kb on + strand, within CA265_RS19030 at 4734.788 kb on + strand, within CA265_RS19030 at 4734.794 kb on - strand, within CA265_RS19030 at 4734.924 kb on + strand, within CA265_RS19030 at 4734.924 kb on + strand, within CA265_RS19030 at 4734.924 kb on + strand, within CA265_RS19030 at 4734.982 kb on + strand, within CA265_RS19030 at 4734.982 kb on + strand, within CA265_RS19030 at 4734.983 kb on - strand, within CA265_RS19030 at 4734.983 kb on - strand, within CA265_RS19030 at 4734.991 kb on - strand, within CA265_RS19030 at 4735.029 kb on + strand, within CA265_RS19030 at 4735.081 kb on - strand, within CA265_RS19030 at 4735.124 kb on - strand, within CA265_RS19030 at 4735.181 kb on - strand, within CA265_RS19030 at 4735.208 kb on - strand, within CA265_RS19030 at 4735.237 kb on - strand, within CA265_RS19030 at 4735.290 kb on + strand, within CA265_RS19030 at 4735.290 kb on + strand, within CA265_RS19030 at 4735.348 kb on - strand at 4735.427 kb on + strand at 4735.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,733,201 + -0.1 4,733,264 + +1.3 4,733,265 - -1.3 4,733,296 - CA265_RS19025 0.12 -0.9 4,733,296 - CA265_RS19025 0.12 -3.1 4,733,349 - CA265_RS19025 0.16 -1.9 4,733,369 - CA265_RS19025 0.18 +0.5 4,733,411 - CA265_RS19025 0.21 -3.2 4,733,411 - CA265_RS19025 0.21 -0.6 4,733,453 - CA265_RS19025 0.24 -0.9 4,733,501 + CA265_RS19025 0.28 -1.9 4,733,502 - CA265_RS19025 0.28 -0.9 4,733,561 - CA265_RS19025 0.33 -0.4 4,733,600 - CA265_RS19025 0.36 -0.6 4,733,612 - CA265_RS19025 0.37 -2.6 4,733,612 - CA265_RS19025 0.37 +1.1 4,733,616 - CA265_RS19025 0.37 -0.3 4,733,779 + CA265_RS19025 0.49 -0.4 4,733,846 - CA265_RS19025 0.55 -0.5 4,733,851 + CA265_RS19025 0.55 -1.7 4,733,906 - CA265_RS19025 0.59 +0.1 4,733,914 + CA265_RS19025 0.60 -0.6 4,733,953 + CA265_RS19025 0.63 +0.1 4,733,964 + CA265_RS19025 0.64 +0.3 4,733,992 + CA265_RS19025 0.66 -3.6 4,734,003 - CA265_RS19025 0.67 -2.6 4,734,112 - CA265_RS19025 0.75 -0.1 4,734,153 - CA265_RS19025 0.78 +1.7 4,734,153 - CA265_RS19025 0.78 -0.6 4,734,266 + CA265_RS19025 0.87 -2.9 4,734,269 - CA265_RS19025 0.87 +0.5 4,734,269 - CA265_RS19025 0.87 -0.4 4,734,332 + +0.0 4,734,333 - +0.6 4,734,335 - +0.7 4,734,559 + +1.1 4,734,563 + +0.4 4,734,564 - +0.6 4,734,565 + +1.8 4,734,565 + +0.3 4,734,565 + +0.2 4,734,566 - -0.4 4,734,570 + -0.6 4,734,570 + -0.6 4,734,574 + -0.3 4,734,605 + +0.1 4,734,625 + -0.2 4,734,652 - -1.2 4,734,697 + -0.1 4,734,751 - CA265_RS19030 0.17 +0.0 4,734,757 + CA265_RS19030 0.17 +0.3 4,734,788 + CA265_RS19030 0.21 -0.4 4,734,794 - CA265_RS19030 0.22 +0.3 4,734,924 + CA265_RS19030 0.39 -0.1 4,734,924 + CA265_RS19030 0.39 +0.3 4,734,924 + CA265_RS19030 0.39 +0.6 4,734,982 + CA265_RS19030 0.46 +0.7 4,734,982 + CA265_RS19030 0.46 -0.7 4,734,983 - CA265_RS19030 0.46 -0.3 4,734,983 - CA265_RS19030 0.46 -0.9 4,734,991 - CA265_RS19030 0.47 +0.2 4,735,029 + CA265_RS19030 0.52 +0.4 4,735,081 - CA265_RS19030 0.58 -0.1 4,735,124 - CA265_RS19030 0.64 -0.4 4,735,181 - CA265_RS19030 0.71 +0.3 4,735,208 - CA265_RS19030 0.74 -2.8 4,735,237 - CA265_RS19030 0.78 -0.3 4,735,290 + CA265_RS19030 0.85 +0.3 4,735,290 + CA265_RS19030 0.85 -1.2 4,735,348 - -0.1 4,735,427 + +1.2 4,735,428 - +0.2
Or see this region's nucleotide sequence