Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18015 and CA265_RS18020 overlap by 23 nucleotides CA265_RS18020 and CA265_RS18025 are separated by 41 nucleotides CA265_RS18025 and CA265_RS18030 are separated by 155 nucleotides
CA265_RS18015: CA265_RS18015 - glycosyl transferase, at 4,501,551 to 4,502,687
_RS18015
CA265_RS18020: CA265_RS18020 - hypothetical protein, at 4,502,665 to 4,503,642
_RS18020
CA265_RS18025: CA265_RS18025 - crossover junction endodeoxyribonuclease RuvC, at 4,503,684 to 4,504,271
_RS18025
CA265_RS18030: CA265_RS18030 - hypothetical protein, at 4,504,427 to 4,505,299
_RS18030
Position (kb)
4502
4503
4504 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4501.686 kb on + strand, within CA265_RS18015 at 4501.687 kb on - strand, within CA265_RS18015 at 4501.735 kb on + strand, within CA265_RS18015 at 4501.793 kb on + strand, within CA265_RS18015 at 4501.793 kb on + strand, within CA265_RS18015 at 4501.827 kb on + strand, within CA265_RS18015 at 4501.889 kb on + strand, within CA265_RS18015 at 4501.890 kb on - strand, within CA265_RS18015 at 4501.899 kb on + strand, within CA265_RS18015 at 4501.919 kb on + strand, within CA265_RS18015 at 4501.920 kb on - strand, within CA265_RS18015 at 4501.921 kb on + strand, within CA265_RS18015 at 4501.923 kb on + strand, within CA265_RS18015 at 4501.923 kb on + strand, within CA265_RS18015 at 4501.923 kb on + strand, within CA265_RS18015 at 4501.924 kb on - strand, within CA265_RS18015 at 4501.967 kb on - strand, within CA265_RS18015 at 4501.989 kb on + strand, within CA265_RS18015 at 4501.990 kb on - strand, within CA265_RS18015 at 4502.085 kb on - strand, within CA265_RS18015 at 4502.140 kb on - strand, within CA265_RS18015 at 4502.140 kb on - strand, within CA265_RS18015 at 4502.285 kb on + strand, within CA265_RS18015 at 4502.288 kb on + strand, within CA265_RS18015 at 4502.317 kb on - strand, within CA265_RS18015 at 4502.402 kb on - strand, within CA265_RS18015 at 4502.402 kb on - strand, within CA265_RS18015 at 4502.402 kb on - strand, within CA265_RS18015 at 4502.447 kb on + strand, within CA265_RS18015 at 4502.471 kb on + strand, within CA265_RS18015 at 4502.556 kb on - strand, within CA265_RS18015 at 4502.640 kb on + strand at 4502.661 kb on - strand at 4502.661 kb on - strand at 4502.711 kb on + strand at 4502.711 kb on + strand at 4502.720 kb on - strand at 4502.720 kb on - strand at 4502.720 kb on - strand at 4502.724 kb on + strand at 4502.735 kb on - strand at 4502.744 kb on - strand at 4502.752 kb on + strand at 4502.752 kb on + strand at 4502.752 kb on + strand at 4502.754 kb on + strand at 4502.754 kb on + strand at 4502.755 kb on - strand at 4502.779 kb on + strand, within CA265_RS18020 at 4502.837 kb on - strand, within CA265_RS18020 at 4502.902 kb on - strand, within CA265_RS18020 at 4502.913 kb on - strand, within CA265_RS18020 at 4502.966 kb on - strand, within CA265_RS18020 at 4502.988 kb on - strand, within CA265_RS18020 at 4502.988 kb on - strand, within CA265_RS18020 at 4503.089 kb on - strand, within CA265_RS18020 at 4503.108 kb on - strand, within CA265_RS18020 at 4503.148 kb on + strand, within CA265_RS18020 at 4503.233 kb on - strand, within CA265_RS18020 at 4503.235 kb on + strand, within CA265_RS18020 at 4503.422 kb on + strand, within CA265_RS18020 at 4503.510 kb on + strand, within CA265_RS18020 at 4503.666 kb on - strand at 4503.674 kb on + strand at 4503.682 kb on + strand at 4503.682 kb on + strand at 4503.682 kb on + strand at 4503.940 kb on + strand at 4504.339 kb on - strand at 4504.400 kb on + strand at 4504.476 kb on + strand at 4504.481 kb on + strand at 4504.514 kb on + strand at 4504.523 kb on + strand, within CA265_RS18030 at 4504.528 kb on + strand, within CA265_RS18030 at 4504.532 kb on + strand, within CA265_RS18030 at 4504.534 kb on + strand, within CA265_RS18030 at 4504.541 kb on + strand, within CA265_RS18030 at 4504.617 kb on + strand, within CA265_RS18030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,501,686 + CA265_RS18015 0.12 -0.4 4,501,687 - CA265_RS18015 0.12 +0.9 4,501,735 + CA265_RS18015 0.16 +0.9 4,501,793 + CA265_RS18015 0.21 -2.9 4,501,793 + CA265_RS18015 0.21 -1.0 4,501,827 + CA265_RS18015 0.24 -0.7 4,501,889 + CA265_RS18015 0.30 -0.7 4,501,890 - CA265_RS18015 0.30 -0.1 4,501,899 + CA265_RS18015 0.31 -1.7 4,501,919 + CA265_RS18015 0.32 -0.6 4,501,920 - CA265_RS18015 0.32 -1.6 4,501,921 + CA265_RS18015 0.33 -0.3 4,501,923 + CA265_RS18015 0.33 +0.3 4,501,923 + CA265_RS18015 0.33 -1.1 4,501,923 + CA265_RS18015 0.33 -0.9 4,501,924 - CA265_RS18015 0.33 -1.0 4,501,967 - CA265_RS18015 0.37 -0.2 4,501,989 + CA265_RS18015 0.39 -0.7 4,501,990 - CA265_RS18015 0.39 -1.1 4,502,085 - CA265_RS18015 0.47 -0.7 4,502,140 - CA265_RS18015 0.52 -3.4 4,502,140 - CA265_RS18015 0.52 -0.7 4,502,285 + CA265_RS18015 0.65 -0.3 4,502,288 + CA265_RS18015 0.65 -1.4 4,502,317 - CA265_RS18015 0.67 -1.8 4,502,402 - CA265_RS18015 0.75 -2.0 4,502,402 - CA265_RS18015 0.75 -0.5 4,502,402 - CA265_RS18015 0.75 -1.2 4,502,447 + CA265_RS18015 0.79 -0.1 4,502,471 + CA265_RS18015 0.81 -0.9 4,502,556 - CA265_RS18015 0.88 -1.0 4,502,640 + -0.1 4,502,661 - -1.5 4,502,661 - +0.1 4,502,711 + -0.8 4,502,711 + -0.9 4,502,720 - -0.2 4,502,720 - -1.1 4,502,720 - -0.1 4,502,724 + -0.4 4,502,735 - -0.8 4,502,744 - -0.5 4,502,752 + -2.2 4,502,752 + -0.4 4,502,752 + -1.7 4,502,754 + -0.4 4,502,754 + -0.7 4,502,755 - -2.5 4,502,779 + CA265_RS18020 0.12 -2.6 4,502,837 - CA265_RS18020 0.18 -0.2 4,502,902 - CA265_RS18020 0.24 -1.3 4,502,913 - CA265_RS18020 0.25 -1.7 4,502,966 - CA265_RS18020 0.31 -2.2 4,502,988 - CA265_RS18020 0.33 -1.4 4,502,988 - CA265_RS18020 0.33 -0.5 4,503,089 - CA265_RS18020 0.43 -1.3 4,503,108 - CA265_RS18020 0.45 +0.3 4,503,148 + CA265_RS18020 0.49 -1.2 4,503,233 - CA265_RS18020 0.58 -0.5 4,503,235 + CA265_RS18020 0.58 -1.1 4,503,422 + CA265_RS18020 0.77 -0.4 4,503,510 + CA265_RS18020 0.86 -0.3 4,503,666 - -0.4 4,503,674 + -0.3 4,503,682 + -0.2 4,503,682 + +0.2 4,503,682 + +0.2 4,503,940 + +0.0 4,504,339 - +0.1 4,504,400 + -0.3 4,504,476 + +0.6 4,504,481 + +0.3 4,504,514 + -1.0 4,504,523 + CA265_RS18030 0.11 -0.2 4,504,528 + CA265_RS18030 0.12 +0.5 4,504,532 + CA265_RS18030 0.12 -0.5 4,504,534 + CA265_RS18030 0.12 -0.1 4,504,541 + CA265_RS18030 0.13 -1.0 4,504,617 + CA265_RS18030 0.22 +0.2
Or see this region's nucleotide sequence