Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17770 and CA265_RS17775 are separated by 62 nucleotides CA265_RS17775 and CA265_RS17780 are separated by 182 nucleotides CA265_RS17780 and CA265_RS17785 are separated by 52 nucleotides
CA265_RS17770: CA265_RS17770 - hypothetical protein, at 4,447,223 to 4,448,383
_RS17770
CA265_RS17775: CA265_RS17775 - hypothetical protein, at 4,448,446 to 4,449,045
_RS17775
CA265_RS17780: CA265_RS17780 - hypothetical protein, at 4,449,228 to 4,449,770
_RS17780
CA265_RS17785: CA265_RS17785 - hypothetical protein, at 4,449,823 to 4,450,518
_RS17785
Position (kb)
4448
4449
4450 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4447.509 kb on - strand, within CA265_RS17770 at 4447.572 kb on - strand, within CA265_RS17770 at 4447.586 kb on + strand, within CA265_RS17770 at 4447.587 kb on - strand, within CA265_RS17770 at 4447.587 kb on - strand, within CA265_RS17770 at 4447.616 kb on - strand, within CA265_RS17770 at 4447.617 kb on + strand, within CA265_RS17770 at 4447.618 kb on - strand, within CA265_RS17770 at 4447.625 kb on - strand, within CA265_RS17770 at 4447.627 kb on + strand, within CA265_RS17770 at 4447.678 kb on + strand, within CA265_RS17770 at 4447.700 kb on - strand, within CA265_RS17770 at 4447.704 kb on - strand, within CA265_RS17770 at 4447.771 kb on - strand, within CA265_RS17770 at 4447.878 kb on - strand, within CA265_RS17770 at 4447.896 kb on - strand, within CA265_RS17770 at 4447.900 kb on + strand, within CA265_RS17770 at 4447.903 kb on - strand, within CA265_RS17770 at 4448.054 kb on - strand, within CA265_RS17770 at 4448.104 kb on + strand, within CA265_RS17770 at 4448.133 kb on - strand, within CA265_RS17770 at 4448.137 kb on + strand, within CA265_RS17770 at 4448.137 kb on + strand, within CA265_RS17770 at 4448.144 kb on + strand, within CA265_RS17770 at 4448.341 kb on + strand at 4448.347 kb on + strand at 4448.365 kb on - strand at 4448.369 kb on + strand at 4448.370 kb on - strand at 4448.372 kb on - strand at 4448.399 kb on - strand at 4448.420 kb on - strand at 4448.537 kb on - strand, within CA265_RS17775 at 4448.568 kb on + strand, within CA265_RS17775 at 4448.569 kb on - strand, within CA265_RS17775 at 4448.602 kb on - strand, within CA265_RS17775 at 4448.665 kb on - strand, within CA265_RS17775 at 4448.710 kb on - strand, within CA265_RS17775 at 4448.712 kb on + strand, within CA265_RS17775 at 4448.812 kb on - strand, within CA265_RS17775 at 4448.828 kb on - strand, within CA265_RS17775 at 4448.837 kb on - strand, within CA265_RS17775 at 4448.945 kb on - strand, within CA265_RS17775 at 4448.953 kb on + strand, within CA265_RS17775 at 4448.954 kb on + strand, within CA265_RS17775 at 4448.955 kb on - strand, within CA265_RS17775 at 4449.015 kb on + strand at 4449.015 kb on + strand at 4449.016 kb on - strand at 4449.028 kb on + strand at 4449.046 kb on - strand at 4449.072 kb on - strand at 4449.079 kb on + strand at 4449.079 kb on + strand at 4449.082 kb on - strand at 4449.103 kb on - strand at 4449.143 kb on + strand at 4449.191 kb on - strand at 4449.232 kb on + strand at 4449.232 kb on + strand at 4449.848 kb on - strand at 4449.866 kb on - strand at 4449.922 kb on - strand, within CA265_RS17785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,447,509 - CA265_RS17770 0.25 -3.8 4,447,572 - CA265_RS17770 0.30 -1.8 4,447,586 + CA265_RS17770 0.31 +1.1 4,447,587 - CA265_RS17770 0.31 -2.7 4,447,587 - CA265_RS17770 0.31 -2.0 4,447,616 - CA265_RS17770 0.34 -3.2 4,447,617 + CA265_RS17770 0.34 +0.1 4,447,618 - CA265_RS17770 0.34 -3.4 4,447,625 - CA265_RS17770 0.35 -2.5 4,447,627 + CA265_RS17770 0.35 +0.3 4,447,678 + CA265_RS17770 0.39 +0.0 4,447,700 - CA265_RS17770 0.41 -1.5 4,447,704 - CA265_RS17770 0.41 -1.8 4,447,771 - CA265_RS17770 0.47 -1.2 4,447,878 - CA265_RS17770 0.56 -3.0 4,447,896 - CA265_RS17770 0.58 -2.3 4,447,900 + CA265_RS17770 0.58 +0.9 4,447,903 - CA265_RS17770 0.59 -3.2 4,448,054 - CA265_RS17770 0.72 -1.5 4,448,104 + CA265_RS17770 0.76 +0.0 4,448,133 - CA265_RS17770 0.78 -2.1 4,448,137 + CA265_RS17770 0.79 -0.2 4,448,137 + CA265_RS17770 0.79 +0.5 4,448,144 + CA265_RS17770 0.79 +0.9 4,448,341 + -0.1 4,448,347 + +1.4 4,448,365 - -3.2 4,448,369 + +0.4 4,448,370 - +1.6 4,448,372 - -3.3 4,448,399 - -2.3 4,448,420 - -1.7 4,448,537 - CA265_RS17775 0.15 -2.2 4,448,568 + CA265_RS17775 0.20 -0.8 4,448,569 - CA265_RS17775 0.20 -4.2 4,448,602 - CA265_RS17775 0.26 -2.6 4,448,665 - CA265_RS17775 0.36 -1.6 4,448,710 - CA265_RS17775 0.44 +0.6 4,448,712 + CA265_RS17775 0.44 -0.6 4,448,812 - CA265_RS17775 0.61 -0.4 4,448,828 - CA265_RS17775 0.64 -1.0 4,448,837 - CA265_RS17775 0.65 -1.1 4,448,945 - CA265_RS17775 0.83 -0.5 4,448,953 + CA265_RS17775 0.84 +0.2 4,448,954 + CA265_RS17775 0.85 -0.3 4,448,955 - CA265_RS17775 0.85 -0.0 4,449,015 + +1.0 4,449,015 + +1.1 4,449,016 - +0.6 4,449,028 + +1.3 4,449,046 - +1.3 4,449,072 - -0.6 4,449,079 + +0.6 4,449,079 + +0.8 4,449,082 - -1.5 4,449,103 - -1.8 4,449,143 + -0.3 4,449,191 - -1.6 4,449,232 + +0.2 4,449,232 + +0.9 4,449,848 - -0.6 4,449,866 - -1.1 4,449,922 - CA265_RS17785 0.14 +0.6
Or see this region's nucleotide sequence