Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17450 and CA265_RS17455 are separated by 6 nucleotides CA265_RS17455 and CA265_RS17460 are separated by 4 nucleotides CA265_RS17460 and CA265_RS17465 are separated by 49 nucleotides
CA265_RS17450: CA265_RS17450 - hypothetical protein, at 4,351,963 to 4,353,819
_RS17450
CA265_RS17455: CA265_RS17455 - hypothetical protein, at 4,353,826 to 4,354,776
_RS17455
CA265_RS17460: CA265_RS17460 - RNA polymerase sigma-70 factor, at 4,354,781 to 4,355,347
_RS17460
CA265_RS17465: CA265_RS17465 - hypothetical protein, at 4,355,397 to 4,356,188
_RS17465
Position (kb)
4353
4354
4355 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4352.829 kb on + strand, within CA265_RS17450 at 4352.829 kb on + strand, within CA265_RS17450 at 4352.888 kb on - strand at 4352.911 kb on - strand, within CA265_RS17450 at 4352.927 kb on - strand, within CA265_RS17450 at 4352.960 kb on - strand, within CA265_RS17450 at 4353.018 kb on + strand at 4353.028 kb on + strand, within CA265_RS17450 at 4353.086 kb on - strand, within CA265_RS17450 at 4353.127 kb on - strand, within CA265_RS17450 at 4353.177 kb on + strand, within CA265_RS17450 at 4353.177 kb on + strand, within CA265_RS17450 at 4353.178 kb on - strand, within CA265_RS17450 at 4353.199 kb on + strand, within CA265_RS17450 at 4353.222 kb on + strand, within CA265_RS17450 at 4353.256 kb on - strand, within CA265_RS17450 at 4353.294 kb on + strand, within CA265_RS17450 at 4353.295 kb on - strand, within CA265_RS17450 at 4353.297 kb on - strand, within CA265_RS17450 at 4353.343 kb on + strand, within CA265_RS17450 at 4353.405 kb on - strand, within CA265_RS17450 at 4353.408 kb on + strand, within CA265_RS17450 at 4353.408 kb on + strand, within CA265_RS17450 at 4353.408 kb on + strand, within CA265_RS17450 at 4353.409 kb on - strand, within CA265_RS17450 at 4353.448 kb on + strand, within CA265_RS17450 at 4353.448 kb on + strand, within CA265_RS17450 at 4353.467 kb on + strand, within CA265_RS17450 at 4353.467 kb on + strand, within CA265_RS17450 at 4353.524 kb on + strand, within CA265_RS17450 at 4353.525 kb on - strand, within CA265_RS17450 at 4353.525 kb on - strand, within CA265_RS17450 at 4353.567 kb on + strand, within CA265_RS17450 at 4353.658 kb on - strand at 4353.659 kb on + strand at 4353.669 kb on + strand at 4353.689 kb on - strand at 4353.689 kb on - strand at 4353.766 kb on + strand at 4353.766 kb on + strand at 4353.770 kb on - strand at 4353.802 kb on - strand at 4353.813 kb on + strand at 4353.814 kb on - strand at 4353.831 kb on + strand at 4353.970 kb on - strand, within CA265_RS17455 at 4354.052 kb on + strand, within CA265_RS17455 at 4354.181 kb on - strand, within CA265_RS17455 at 4354.254 kb on + strand, within CA265_RS17455 at 4354.255 kb on - strand, within CA265_RS17455 at 4354.255 kb on - strand, within CA265_RS17455 at 4354.260 kb on + strand, within CA265_RS17455 at 4354.467 kb on - strand, within CA265_RS17455 at 4354.475 kb on - strand, within CA265_RS17455 at 4354.530 kb on - strand, within CA265_RS17455 at 4354.618 kb on - strand at 4354.648 kb on + strand, within CA265_RS17455 at 4354.648 kb on + strand, within CA265_RS17455 at 4354.714 kb on - strand at 4354.776 kb on + strand at 4354.833 kb on - strand at 4354.883 kb on - strand, within CA265_RS17460 at 4355.158 kb on + strand, within CA265_RS17460 at 4355.187 kb on - strand, within CA265_RS17460 at 4355.234 kb on + strand, within CA265_RS17460 at 4355.234 kb on + strand, within CA265_RS17460 at 4355.248 kb on - strand, within CA265_RS17460 at 4355.300 kb on - strand at 4355.459 kb on + strand at 4355.459 kb on + strand at 4355.479 kb on - strand, within CA265_RS17465 at 4355.502 kb on + strand, within CA265_RS17465 at 4355.535 kb on + strand, within CA265_RS17465 at 4355.535 kb on + strand, within CA265_RS17465 at 4355.536 kb on - strand, within CA265_RS17465 at 4355.565 kb on + strand, within CA265_RS17465 at 4355.625 kb on - strand, within CA265_RS17465 at 4355.631 kb on + strand, within CA265_RS17465 at 4355.658 kb on - strand, within CA265_RS17465 at 4355.658 kb on - strand, within CA265_RS17465 at 4355.669 kb on + strand, within CA265_RS17465 at 4355.731 kb on + strand, within CA265_RS17465
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,352,829 + CA265_RS17450 0.47 -0.1 4,352,829 + CA265_RS17450 0.47 +0.5 4,352,888 - -0.1 4,352,911 - CA265_RS17450 0.51 -1.7 4,352,927 - CA265_RS17450 0.52 -1.4 4,352,960 - CA265_RS17450 0.54 -0.1 4,353,018 + -1.1 4,353,028 + CA265_RS17450 0.57 +1.0 4,353,086 - CA265_RS17450 0.60 -0.2 4,353,127 - CA265_RS17450 0.63 +0.0 4,353,177 + CA265_RS17450 0.65 +0.1 4,353,177 + CA265_RS17450 0.65 -0.4 4,353,178 - CA265_RS17450 0.65 -1.6 4,353,199 + CA265_RS17450 0.67 -0.2 4,353,222 + CA265_RS17450 0.68 -0.2 4,353,256 - CA265_RS17450 0.70 +0.4 4,353,294 + CA265_RS17450 0.72 -0.4 4,353,295 - CA265_RS17450 0.72 +0.5 4,353,297 - CA265_RS17450 0.72 -2.0 4,353,343 + CA265_RS17450 0.74 -0.2 4,353,405 - CA265_RS17450 0.78 +0.9 4,353,408 + CA265_RS17450 0.78 +0.8 4,353,408 + CA265_RS17450 0.78 -0.2 4,353,408 + CA265_RS17450 0.78 +0.4 4,353,409 - CA265_RS17450 0.78 -0.9 4,353,448 + CA265_RS17450 0.80 +0.1 4,353,448 + CA265_RS17450 0.80 -0.3 4,353,467 + CA265_RS17450 0.81 +1.2 4,353,467 + CA265_RS17450 0.81 -0.1 4,353,524 + CA265_RS17450 0.84 -0.1 4,353,525 - CA265_RS17450 0.84 -0.3 4,353,525 - CA265_RS17450 0.84 +0.2 4,353,567 + CA265_RS17450 0.86 -0.1 4,353,658 - -0.8 4,353,659 + +1.5 4,353,669 + -0.4 4,353,689 - -0.1 4,353,689 - -1.5 4,353,766 + +0.2 4,353,766 + -0.4 4,353,770 - -0.7 4,353,802 - -0.0 4,353,813 + -0.6 4,353,814 - -0.6 4,353,831 + +0.0 4,353,970 - CA265_RS17455 0.15 -1.1 4,354,052 + CA265_RS17455 0.24 -1.8 4,354,181 - CA265_RS17455 0.37 -0.8 4,354,254 + CA265_RS17455 0.45 -3.1 4,354,255 - CA265_RS17455 0.45 -1.2 4,354,255 - CA265_RS17455 0.45 -0.8 4,354,260 + CA265_RS17455 0.46 -2.3 4,354,467 - CA265_RS17455 0.67 -1.0 4,354,475 - CA265_RS17455 0.68 +0.1 4,354,530 - CA265_RS17455 0.74 -0.8 4,354,618 - +0.9 4,354,648 + CA265_RS17455 0.86 -0.5 4,354,648 + CA265_RS17455 0.86 +1.0 4,354,714 - -1.0 4,354,776 + -2.3 4,354,833 - -0.1 4,354,883 - CA265_RS17460 0.18 +0.1 4,355,158 + CA265_RS17460 0.66 -0.2 4,355,187 - CA265_RS17460 0.72 +1.3 4,355,234 + CA265_RS17460 0.80 +0.6 4,355,234 + CA265_RS17460 0.80 +0.1 4,355,248 - CA265_RS17460 0.82 -0.0 4,355,300 - +0.1 4,355,459 + -0.1 4,355,459 + +0.3 4,355,479 - CA265_RS17465 0.10 +0.2 4,355,502 + CA265_RS17465 0.13 +0.3 4,355,535 + CA265_RS17465 0.17 +0.3 4,355,535 + CA265_RS17465 0.17 +0.2 4,355,536 - CA265_RS17465 0.18 +0.4 4,355,565 + CA265_RS17465 0.21 +0.0 4,355,625 - CA265_RS17465 0.29 -0.1 4,355,631 + CA265_RS17465 0.30 -0.2 4,355,658 - CA265_RS17465 0.33 -1.1 4,355,658 - CA265_RS17465 0.33 -1.1 4,355,669 + CA265_RS17465 0.34 +0.2 4,355,731 + CA265_RS17465 0.42 +0.2
Or see this region's nucleotide sequence