Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS16010 and CA265_RS16015 are separated by 146 nucleotides CA265_RS16015 and CA265_RS16020 overlap by 1 nucleotides CA265_RS16020 and CA265_RS16025 are separated by 20 nucleotides CA265_RS16025 and CA265_RS16030 overlap by 1 nucleotides CA265_RS16030 and CA265_RS16035 are separated by 4 nucleotides
CA265_RS16010: CA265_RS16010 - short-chain dehydrogenase, at 4,018,191 to 4,018,880
_RS16010
CA265_RS16015: CA265_RS16015 - ABC transporter permease, at 4,019,027 to 4,019,761
_RS16015
CA265_RS16020: CA265_RS16020 - ABC transporter ATP-binding protein, at 4,019,761 to 4,020,513
_RS16020
CA265_RS16025: CA265_RS16025 - hypothetical protein, at 4,020,534 to 4,020,776
_RS16025
CA265_RS16030: CA265_RS16030 - hypothetical protein, at 4,020,776 to 4,021,159
_RS16030
CA265_RS16035: CA265_RS16035 - oxidoreductase, at 4,021,164 to 4,022,045
_RS16035
Position (kb)
4019
4020
4021 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4018.836 kb on - strand at 4018.886 kb on - strand at 4018.915 kb on + strand at 4018.919 kb on + strand at 4018.921 kb on + strand at 4018.986 kb on + strand at 4018.986 kb on + strand at 4018.987 kb on - strand at 4018.989 kb on + strand at 4018.990 kb on - strand at 4018.990 kb on - strand at 4018.992 kb on - strand at 4019.009 kb on + strand at 4019.018 kb on - strand at 4019.021 kb on + strand at 4019.021 kb on + strand at 4019.051 kb on + strand at 4019.132 kb on + strand, within CA265_RS16015 at 4019.132 kb on + strand, within CA265_RS16015 at 4019.132 kb on + strand, within CA265_RS16015 at 4019.132 kb on + strand, within CA265_RS16015 at 4019.142 kb on + strand, within CA265_RS16015 at 4019.142 kb on + strand, within CA265_RS16015 at 4019.151 kb on + strand, within CA265_RS16015 at 4019.152 kb on - strand, within CA265_RS16015 at 4019.245 kb on + strand, within CA265_RS16015 at 4019.273 kb on - strand, within CA265_RS16015 at 4019.273 kb on - strand, within CA265_RS16015 at 4019.274 kb on + strand, within CA265_RS16015 at 4019.297 kb on - strand, within CA265_RS16015 at 4019.307 kb on + strand, within CA265_RS16015 at 4019.389 kb on + strand, within CA265_RS16015 at 4019.396 kb on - strand, within CA265_RS16015 at 4019.398 kb on - strand, within CA265_RS16015 at 4019.398 kb on - strand, within CA265_RS16015 at 4019.412 kb on - strand, within CA265_RS16015 at 4019.431 kb on + strand, within CA265_RS16015 at 4019.474 kb on - strand, within CA265_RS16015 at 4019.482 kb on - strand, within CA265_RS16015 at 4019.482 kb on - strand, within CA265_RS16015 at 4019.485 kb on + strand, within CA265_RS16015 at 4019.501 kb on - strand, within CA265_RS16015 at 4019.513 kb on - strand, within CA265_RS16015 at 4019.552 kb on - strand, within CA265_RS16015 at 4019.575 kb on + strand, within CA265_RS16015 at 4019.607 kb on + strand, within CA265_RS16015 at 4019.651 kb on + strand, within CA265_RS16015 at 4019.670 kb on + strand, within CA265_RS16015 at 4019.708 kb on - strand at 4019.713 kb on + strand at 4019.713 kb on + strand at 4019.714 kb on - strand at 4019.740 kb on - strand at 4019.778 kb on + strand at 4019.782 kb on + strand at 4019.808 kb on - strand at 4019.810 kb on + strand at 4019.810 kb on + strand at 4019.810 kb on + strand at 4019.853 kb on - strand, within CA265_RS16020 at 4019.856 kb on + strand, within CA265_RS16020 at 4019.857 kb on - strand, within CA265_RS16020 at 4019.888 kb on + strand, within CA265_RS16020 at 4019.898 kb on + strand, within CA265_RS16020 at 4019.899 kb on - strand, within CA265_RS16020 at 4019.899 kb on - strand, within CA265_RS16020 at 4019.938 kb on + strand, within CA265_RS16020 at 4020.008 kb on + strand, within CA265_RS16020 at 4020.037 kb on - strand, within CA265_RS16020 at 4020.037 kb on - strand, within CA265_RS16020 at 4020.072 kb on + strand, within CA265_RS16020 at 4020.073 kb on - strand, within CA265_RS16020 at 4020.073 kb on - strand, within CA265_RS16020 at 4020.173 kb on + strand, within CA265_RS16020 at 4020.233 kb on - strand, within CA265_RS16020 at 4020.334 kb on - strand, within CA265_RS16020 at 4020.351 kb on + strand, within CA265_RS16020 at 4020.351 kb on + strand, within CA265_RS16020 at 4020.352 kb on - strand, within CA265_RS16020 at 4020.352 kb on - strand, within CA265_RS16020 at 4020.380 kb on - strand, within CA265_RS16020 at 4020.441 kb on + strand at 4020.531 kb on + strand at 4020.699 kb on + strand, within CA265_RS16025 at 4020.699 kb on + strand, within CA265_RS16025 at 4020.723 kb on - strand, within CA265_RS16025 at 4020.769 kb on - strand at 4020.770 kb on + strand at 4020.774 kb on + strand at 4020.775 kb on - strand at 4020.775 kb on - strand at 4020.775 kb on - strand at 4020.793 kb on + strand at 4020.799 kb on + strand at 4020.827 kb on - strand, within CA265_RS16030 at 4020.841 kb on - strand, within CA265_RS16030 at 4020.849 kb on + strand, within CA265_RS16030 at 4020.850 kb on - strand, within CA265_RS16030 at 4020.878 kb on - strand, within CA265_RS16030 at 4020.885 kb on + strand, within CA265_RS16030 at 4020.927 kb on + strand, within CA265_RS16030 at 4020.937 kb on - strand, within CA265_RS16030 at 4020.965 kb on + strand, within CA265_RS16030 at 4020.965 kb on + strand, within CA265_RS16030 at 4020.965 kb on + strand, within CA265_RS16030 at 4020.966 kb on - strand, within CA265_RS16030 at 4020.966 kb on - strand, within CA265_RS16030 at 4021.005 kb on + strand, within CA265_RS16030 at 4021.006 kb on - strand, within CA265_RS16030 at 4021.028 kb on + strand, within CA265_RS16030 at 4021.028 kb on + strand, within CA265_RS16030 at 4021.033 kb on - strand, within CA265_RS16030 at 4021.096 kb on + strand, within CA265_RS16030 at 4021.111 kb on - strand, within CA265_RS16030 at 4021.124 kb on + strand at 4021.125 kb on - strand at 4021.158 kb on - strand at 4021.161 kb on + strand at 4021.174 kb on + strand at 4021.175 kb on - strand at 4021.175 kb on - strand at 4021.253 kb on - strand, within CA265_RS16035 at 4021.328 kb on + strand, within CA265_RS16035 at 4021.352 kb on + strand, within CA265_RS16035 at 4021.405 kb on + strand, within CA265_RS16035 at 4021.405 kb on + strand, within CA265_RS16035 at 4021.406 kb on - strand, within CA265_RS16035 at 4021.413 kb on - strand, within CA265_RS16035 at 4021.433 kb on + strand, within CA265_RS16035 at 4021.434 kb on - strand, within CA265_RS16035 at 4021.444 kb on + strand, within CA265_RS16035 at 4021.451 kb on - strand, within CA265_RS16035 at 4021.468 kb on + strand, within CA265_RS16035 at 4021.493 kb on - strand, within CA265_RS16035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 4,018,836 - +0.0 4,018,886 - -0.4 4,018,915 + -1.2 4,018,919 + +0.5 4,018,921 + -0.1 4,018,986 + -1.6 4,018,986 + +0.1 4,018,987 - -0.3 4,018,989 + +0.0 4,018,990 - -0.7 4,018,990 - -2.0 4,018,992 - +0.6 4,019,009 + +0.1 4,019,018 - -3.0 4,019,021 + -0.1 4,019,021 + -0.5 4,019,051 + +0.0 4,019,132 + CA265_RS16015 0.14 -0.8 4,019,132 + CA265_RS16015 0.14 -1.6 4,019,132 + CA265_RS16015 0.14 +0.4 4,019,132 + CA265_RS16015 0.14 -0.3 4,019,142 + CA265_RS16015 0.16 -0.7 4,019,142 + CA265_RS16015 0.16 -1.6 4,019,151 + CA265_RS16015 0.17 -0.7 4,019,152 - CA265_RS16015 0.17 +0.5 4,019,245 + CA265_RS16015 0.30 -3.1 4,019,273 - CA265_RS16015 0.33 -1.6 4,019,273 - CA265_RS16015 0.33 -3.4 4,019,274 + CA265_RS16015 0.34 -0.6 4,019,297 - CA265_RS16015 0.37 -1.6 4,019,307 + CA265_RS16015 0.38 -0.3 4,019,389 + CA265_RS16015 0.49 +0.3 4,019,396 - CA265_RS16015 0.50 -1.2 4,019,398 - CA265_RS16015 0.50 -0.7 4,019,398 - CA265_RS16015 0.50 -1.7 4,019,412 - CA265_RS16015 0.52 -0.0 4,019,431 + CA265_RS16015 0.55 -0.9 4,019,474 - CA265_RS16015 0.61 -1.4 4,019,482 - CA265_RS16015 0.62 +1.4 4,019,482 - CA265_RS16015 0.62 -1.1 4,019,485 + CA265_RS16015 0.62 -1.7 4,019,501 - CA265_RS16015 0.64 -0.5 4,019,513 - CA265_RS16015 0.66 -1.2 4,019,552 - CA265_RS16015 0.71 -0.6 4,019,575 + CA265_RS16015 0.75 -1.2 4,019,607 + CA265_RS16015 0.79 -1.2 4,019,651 + CA265_RS16015 0.85 -1.4 4,019,670 + CA265_RS16015 0.87 -2.3 4,019,708 - -2.3 4,019,713 + -1.6 4,019,713 + -1.0 4,019,714 - -3.0 4,019,740 - -1.4 4,019,778 + +0.1 4,019,782 + -2.0 4,019,808 - -1.3 4,019,810 + -1.1 4,019,810 + -0.8 4,019,810 + +0.2 4,019,853 - CA265_RS16020 0.12 -1.2 4,019,856 + CA265_RS16020 0.13 -1.9 4,019,857 - CA265_RS16020 0.13 -1.7 4,019,888 + CA265_RS16020 0.17 -0.9 4,019,898 + CA265_RS16020 0.18 +0.4 4,019,899 - CA265_RS16020 0.18 -0.7 4,019,899 - CA265_RS16020 0.18 -0.8 4,019,938 + CA265_RS16020 0.24 +0.1 4,020,008 + CA265_RS16020 0.33 -1.7 4,020,037 - CA265_RS16020 0.37 -0.4 4,020,037 - CA265_RS16020 0.37 -0.2 4,020,072 + CA265_RS16020 0.41 -1.5 4,020,073 - CA265_RS16020 0.41 -0.8 4,020,073 - CA265_RS16020 0.41 -0.0 4,020,173 + CA265_RS16020 0.55 -1.2 4,020,233 - CA265_RS16020 0.63 +0.2 4,020,334 - CA265_RS16020 0.76 -0.9 4,020,351 + CA265_RS16020 0.78 -0.8 4,020,351 + CA265_RS16020 0.78 -1.7 4,020,352 - CA265_RS16020 0.78 -0.7 4,020,352 - CA265_RS16020 0.78 -1.2 4,020,380 - CA265_RS16020 0.82 -1.0 4,020,441 + -1.8 4,020,531 + -0.5 4,020,699 + CA265_RS16025 0.68 -0.7 4,020,699 + CA265_RS16025 0.68 -0.3 4,020,723 - CA265_RS16025 0.78 +0.7 4,020,769 - +0.4 4,020,770 + -0.7 4,020,774 + -0.1 4,020,775 - +0.0 4,020,775 - +0.2 4,020,775 - +0.1 4,020,793 + -0.4 4,020,799 + -1.0 4,020,827 - CA265_RS16030 0.13 +0.3 4,020,841 - CA265_RS16030 0.17 +0.9 4,020,849 + CA265_RS16030 0.19 -0.4 4,020,850 - CA265_RS16030 0.19 +0.7 4,020,878 - CA265_RS16030 0.27 +0.5 4,020,885 + CA265_RS16030 0.28 -0.2 4,020,927 + CA265_RS16030 0.39 -0.1 4,020,937 - CA265_RS16030 0.42 +0.1 4,020,965 + CA265_RS16030 0.49 +0.4 4,020,965 + CA265_RS16030 0.49 -0.9 4,020,965 + CA265_RS16030 0.49 -0.6 4,020,966 - CA265_RS16030 0.49 +0.7 4,020,966 - CA265_RS16030 0.49 -0.3 4,021,005 + CA265_RS16030 0.60 -0.4 4,021,006 - CA265_RS16030 0.60 +0.2 4,021,028 + CA265_RS16030 0.66 -0.1 4,021,028 + CA265_RS16030 0.66 +0.2 4,021,033 - CA265_RS16030 0.67 +0.6 4,021,096 + CA265_RS16030 0.83 +0.7 4,021,111 - CA265_RS16030 0.87 -0.6 4,021,124 + +0.2 4,021,125 - +0.2 4,021,158 - +0.2 4,021,161 + +0.0 4,021,174 + -0.3 4,021,175 - -0.0 4,021,175 - +0.0 4,021,253 - CA265_RS16035 0.10 -0.8 4,021,328 + CA265_RS16035 0.19 +0.5 4,021,352 + CA265_RS16035 0.21 +0.2 4,021,405 + CA265_RS16035 0.27 -0.4 4,021,405 + CA265_RS16035 0.27 +0.4 4,021,406 - CA265_RS16035 0.27 +0.5 4,021,413 - CA265_RS16035 0.28 +0.7 4,021,433 + CA265_RS16035 0.30 +0.3 4,021,434 - CA265_RS16035 0.31 +0.5 4,021,444 + CA265_RS16035 0.32 +0.1 4,021,451 - CA265_RS16035 0.33 +0.0 4,021,468 + CA265_RS16035 0.34 -0.5 4,021,493 - CA265_RS16035 0.37 +0.4
Or see this region's nucleotide sequence