Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15850 and CA265_RS15855 are separated by 10 nucleotides CA265_RS15855 and CA265_RS15860 are separated by 270 nucleotides
CA265_RS15850: CA265_RS15850 - 3-isopropylmalate dehydrogenase, at 3,987,438 to 3,988,529
_RS15850
CA265_RS15855: CA265_RS15855 - 2-isopropylmalate synthase (EC 2.3.3.13) (from data) , at 3,988,540 to 3,989,700
_RS15855
CA265_RS15860: CA265_RS15860 - threonine deaminase (EC 4.3.1.19) (from data) , at 3,989,971 to 3,991,221
_RS15860
Position (kb)
3988
3989
3990 Strain fitness (log2 ratio)
-2
-1
0
1 at 3987.672 kb on + strand, within CA265_RS15850 at 3987.687 kb on - strand, within CA265_RS15850 at 3987.687 kb on - strand, within CA265_RS15850 at 3987.743 kb on - strand, within CA265_RS15850 at 3987.897 kb on - strand, within CA265_RS15850 at 3987.928 kb on - strand, within CA265_RS15850 at 3987.951 kb on - strand, within CA265_RS15850 at 3987.978 kb on - strand, within CA265_RS15850 at 3987.989 kb on - strand, within CA265_RS15850 at 3988.002 kb on - strand, within CA265_RS15850 at 3988.215 kb on - strand, within CA265_RS15850 at 3988.320 kb on - strand, within CA265_RS15850 at 3988.320 kb on - strand, within CA265_RS15850 at 3988.336 kb on + strand, within CA265_RS15850 at 3988.343 kb on - strand, within CA265_RS15850 at 3988.389 kb on - strand, within CA265_RS15850 at 3988.430 kb on + strand at 3988.431 kb on - strand at 3988.446 kb on - strand at 3988.567 kb on - strand at 3988.616 kb on + strand at 3988.617 kb on - strand at 3988.721 kb on + strand, within CA265_RS15855 at 3988.722 kb on - strand, within CA265_RS15855 at 3988.725 kb on - strand, within CA265_RS15855 at 3988.755 kb on - strand, within CA265_RS15855 at 3988.812 kb on - strand, within CA265_RS15855 at 3988.930 kb on + strand, within CA265_RS15855 at 3989.109 kb on - strand, within CA265_RS15855 at 3989.223 kb on - strand, within CA265_RS15855 at 3989.263 kb on - strand, within CA265_RS15855 at 3989.379 kb on + strand, within CA265_RS15855 at 3989.379 kb on + strand, within CA265_RS15855 at 3989.431 kb on - strand, within CA265_RS15855 at 3989.520 kb on + strand, within CA265_RS15855 at 3989.521 kb on - strand, within CA265_RS15855 at 3989.567 kb on + strand, within CA265_RS15855 at 3989.583 kb on + strand, within CA265_RS15855 at 3989.604 kb on - strand at 3989.615 kb on - strand at 3989.615 kb on - strand at 3989.617 kb on - strand at 3989.654 kb on + strand at 3989.654 kb on + strand at 3989.655 kb on - strand at 3989.890 kb on + strand at 3989.891 kb on - strand at 3989.891 kb on - strand at 3989.891 kb on - strand at 3989.923 kb on + strand at 3989.923 kb on + strand at 3989.962 kb on + strand at 3989.970 kb on + strand at 3989.977 kb on - strand at 3989.991 kb on + strand at 3989.991 kb on + strand at 3990.024 kb on - strand at 3990.057 kb on + strand at 3990.079 kb on + strand at 3990.079 kb on + strand at 3990.079 kb on + strand at 3990.079 kb on + strand at 3990.090 kb on + strand at 3990.091 kb on - strand at 3990.095 kb on - strand at 3990.098 kb on + strand, within CA265_RS15860 at 3990.259 kb on - strand, within CA265_RS15860 at 3990.274 kb on - strand, within CA265_RS15860 at 3990.304 kb on - strand, within CA265_RS15860 at 3990.348 kb on - strand, within CA265_RS15860 at 3990.391 kb on + strand, within CA265_RS15860 at 3990.393 kb on + strand, within CA265_RS15860 at 3990.394 kb on - strand, within CA265_RS15860 at 3990.470 kb on + strand, within CA265_RS15860 at 3990.470 kb on + strand, within CA265_RS15860 at 3990.502 kb on - strand, within CA265_RS15860 at 3990.546 kb on - strand, within CA265_RS15860 at 3990.553 kb on + strand, within CA265_RS15860 at 3990.554 kb on - strand, within CA265_RS15860 at 3990.556 kb on - strand, within CA265_RS15860 at 3990.556 kb on - strand, within CA265_RS15860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,987,672 + CA265_RS15850 0.21 -1.8 3,987,687 - CA265_RS15850 0.23 -1.0 3,987,687 - CA265_RS15850 0.23 +0.2 3,987,743 - CA265_RS15850 0.28 -0.6 3,987,897 - CA265_RS15850 0.42 -0.8 3,987,928 - CA265_RS15850 0.45 +0.2 3,987,951 - CA265_RS15850 0.47 +0.8 3,987,978 - CA265_RS15850 0.49 -0.5 3,987,989 - CA265_RS15850 0.50 +0.0 3,988,002 - CA265_RS15850 0.52 +0.0 3,988,215 - CA265_RS15850 0.71 -0.2 3,988,320 - CA265_RS15850 0.81 -0.3 3,988,320 - CA265_RS15850 0.81 -0.1 3,988,336 + CA265_RS15850 0.82 +1.4 3,988,343 - CA265_RS15850 0.83 -0.4 3,988,389 - CA265_RS15850 0.87 +0.2 3,988,430 + -1.0 3,988,431 - -0.5 3,988,446 - +0.1 3,988,567 - -0.7 3,988,616 + +0.7 3,988,617 - -0.3 3,988,721 + CA265_RS15855 0.16 -0.9 3,988,722 - CA265_RS15855 0.16 -0.8 3,988,725 - CA265_RS15855 0.16 -0.5 3,988,755 - CA265_RS15855 0.19 -0.1 3,988,812 - CA265_RS15855 0.23 -0.8 3,988,930 + CA265_RS15855 0.34 -0.0 3,989,109 - CA265_RS15855 0.49 -1.3 3,989,223 - CA265_RS15855 0.59 -0.2 3,989,263 - CA265_RS15855 0.62 -0.7 3,989,379 + CA265_RS15855 0.72 -0.9 3,989,379 + CA265_RS15855 0.72 -0.9 3,989,431 - CA265_RS15855 0.77 -0.7 3,989,520 + CA265_RS15855 0.84 -0.1 3,989,521 - CA265_RS15855 0.84 +0.7 3,989,567 + CA265_RS15855 0.88 -0.9 3,989,583 + CA265_RS15855 0.90 -0.6 3,989,604 - +0.2 3,989,615 - +0.2 3,989,615 - -0.3 3,989,617 - +0.1 3,989,654 + -0.2 3,989,654 + +0.8 3,989,655 - +0.4 3,989,890 + -0.9 3,989,891 - -0.5 3,989,891 - -0.4 3,989,891 - -0.1 3,989,923 + +0.1 3,989,923 + -0.4 3,989,962 + -1.4 3,989,970 + -0.4 3,989,977 - -0.2 3,989,991 + -1.1 3,989,991 + -0.8 3,990,024 - -0.1 3,990,057 + -1.4 3,990,079 + +0.2 3,990,079 + -0.8 3,990,079 + -1.2 3,990,079 + +0.4 3,990,090 + -0.7 3,990,091 - -0.6 3,990,095 - +0.4 3,990,098 + CA265_RS15860 0.10 -0.0 3,990,259 - CA265_RS15860 0.23 -0.4 3,990,274 - CA265_RS15860 0.24 -0.0 3,990,304 - CA265_RS15860 0.27 -1.1 3,990,348 - CA265_RS15860 0.30 +0.1 3,990,391 + CA265_RS15860 0.34 -0.7 3,990,393 + CA265_RS15860 0.34 -0.6 3,990,394 - CA265_RS15860 0.34 +0.0 3,990,470 + CA265_RS15860 0.40 +0.3 3,990,470 + CA265_RS15860 0.40 +1.0 3,990,502 - CA265_RS15860 0.42 +0.1 3,990,546 - CA265_RS15860 0.46 +0.2 3,990,553 + CA265_RS15860 0.47 -0.5 3,990,554 - CA265_RS15860 0.47 +0.3 3,990,556 - CA265_RS15860 0.47 +0.3 3,990,556 - CA265_RS15860 0.47 +0.7
Or see this region's nucleotide sequence