Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15030 and CA265_RS15035 overlap by 4 nucleotides CA265_RS15035 and CA265_RS15040 are separated by 153 nucleotides CA265_RS15040 and CA265_RS15045 are separated by 64 nucleotides CA265_RS15045 and CA265_RS15050 are separated by 150 nucleotides
CA265_RS15030: CA265_RS15030 - PIN domain nuclease, at 3,789,283 to 3,789,681
_RS15030
CA265_RS15035: CA265_RS15035 - prevent-host-death protein, at 3,789,678 to 3,789,905
_RS15035
CA265_RS15040: CA265_RS15040 - cytidine deaminase, at 3,790,059 to 3,790,517
_RS15040
CA265_RS15045: CA265_RS15045 - MFS transporter, at 3,790,582 to 3,792,189
_RS15045
CA265_RS15050: CA265_RS15050 - lysine transporter LysE, at 3,792,340 to 3,792,966
_RS15050
Position (kb)
3790
3791
3792
3793 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3789.641 kb on - strand, within CA265_RS15030 at 3789.641 kb on - strand, within CA265_RS15030 at 3789.663 kb on + strand at 3789.740 kb on + strand, within CA265_RS15035 at 3789.839 kb on + strand, within CA265_RS15035 at 3789.991 kb on + strand at 3790.133 kb on + strand, within CA265_RS15040 at 3790.157 kb on - strand, within CA265_RS15040 at 3790.160 kb on - strand, within CA265_RS15040 at 3790.160 kb on - strand, within CA265_RS15040 at 3790.225 kb on + strand, within CA265_RS15040 at 3790.225 kb on + strand, within CA265_RS15040 at 3790.227 kb on + strand, within CA265_RS15040 at 3790.267 kb on + strand, within CA265_RS15040 at 3790.293 kb on + strand, within CA265_RS15040 at 3790.320 kb on - strand, within CA265_RS15040 at 3790.361 kb on + strand, within CA265_RS15040 at 3790.385 kb on + strand, within CA265_RS15040 at 3790.388 kb on - strand, within CA265_RS15040 at 3790.388 kb on - strand, within CA265_RS15040 at 3790.400 kb on + strand, within CA265_RS15040 at 3790.485 kb on - strand at 3790.489 kb on - strand at 3790.620 kb on + strand at 3790.621 kb on - strand at 3790.621 kb on - strand at 3790.670 kb on + strand at 3790.670 kb on + strand at 3790.697 kb on + strand at 3790.773 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.852 kb on - strand, within CA265_RS15045 at 3790.852 kb on - strand, within CA265_RS15045 at 3790.861 kb on + strand, within CA265_RS15045 at 3790.907 kb on - strand, within CA265_RS15045 at 3790.927 kb on + strand, within CA265_RS15045 at 3790.937 kb on - strand, within CA265_RS15045 at 3791.010 kb on + strand, within CA265_RS15045 at 3791.027 kb on + strand, within CA265_RS15045 at 3791.028 kb on - strand, within CA265_RS15045 at 3791.028 kb on - strand, within CA265_RS15045 at 3791.029 kb on + strand, within CA265_RS15045 at 3791.069 kb on - strand, within CA265_RS15045 at 3791.074 kb on - strand, within CA265_RS15045 at 3791.175 kb on + strand, within CA265_RS15045 at 3791.188 kb on + strand, within CA265_RS15045 at 3791.189 kb on - strand, within CA265_RS15045 at 3791.271 kb on + strand, within CA265_RS15045 at 3791.272 kb on - strand, within CA265_RS15045 at 3791.275 kb on + strand, within CA265_RS15045 at 3791.276 kb on - strand, within CA265_RS15045 at 3791.276 kb on - strand, within CA265_RS15045 at 3791.287 kb on + strand, within CA265_RS15045 at 3791.296 kb on - strand, within CA265_RS15045 at 3791.310 kb on + strand, within CA265_RS15045 at 3791.311 kb on - strand, within CA265_RS15045 at 3791.350 kb on - strand, within CA265_RS15045 at 3791.418 kb on - strand, within CA265_RS15045 at 3791.427 kb on + strand, within CA265_RS15045 at 3791.427 kb on + strand, within CA265_RS15045 at 3791.428 kb on - strand, within CA265_RS15045 at 3791.514 kb on + strand, within CA265_RS15045 at 3791.569 kb on + strand, within CA265_RS15045 at 3791.599 kb on + strand, within CA265_RS15045 at 3791.914 kb on - strand, within CA265_RS15045 at 3791.914 kb on - strand, within CA265_RS15045 at 3791.928 kb on + strand, within CA265_RS15045 at 3791.947 kb on + strand, within CA265_RS15045 at 3791.962 kb on - strand, within CA265_RS15045 at 3791.991 kb on - strand, within CA265_RS15045 at 3792.140 kb on - strand at 3792.141 kb on + strand at 3792.148 kb on - strand at 3792.148 kb on - strand at 3792.151 kb on + strand at 3792.288 kb on + strand at 3792.289 kb on - strand at 3792.325 kb on + strand at 3792.325 kb on + strand at 3792.326 kb on - strand at 3792.363 kb on + strand at 3792.379 kb on - strand at 3792.431 kb on + strand, within CA265_RS15050 at 3792.461 kb on + strand, within CA265_RS15050 at 3792.534 kb on + strand, within CA265_RS15050 at 3792.535 kb on - strand, within CA265_RS15050 at 3792.553 kb on + strand, within CA265_RS15050 at 3792.553 kb on + strand, within CA265_RS15050 at 3792.553 kb on + strand, within CA265_RS15050 at 3792.554 kb on - strand, within CA265_RS15050 at 3792.554 kb on - strand, within CA265_RS15050 at 3792.557 kb on + strand, within CA265_RS15050 at 3792.558 kb on - strand, within CA265_RS15050 at 3792.590 kb on + strand, within CA265_RS15050 at 3792.591 kb on - strand, within CA265_RS15050 at 3792.596 kb on + strand, within CA265_RS15050 at 3792.596 kb on + strand, within CA265_RS15050 at 3792.596 kb on + strand, within CA265_RS15050 at 3792.598 kb on + strand, within CA265_RS15050 at 3792.598 kb on + strand, within CA265_RS15050 at 3792.598 kb on + strand, within CA265_RS15050 at 3792.599 kb on - strand, within CA265_RS15050 at 3792.599 kb on - strand, within CA265_RS15050 at 3792.633 kb on + strand, within CA265_RS15050 at 3792.675 kb on - strand, within CA265_RS15050 at 3792.732 kb on - strand, within CA265_RS15050 at 3792.740 kb on + strand, within CA265_RS15050 at 3792.816 kb on + strand, within CA265_RS15050 at 3792.838 kb on + strand, within CA265_RS15050 at 3792.847 kb on - strand, within CA265_RS15050 at 3792.864 kb on - strand, within CA265_RS15050 at 3792.879 kb on + strand, within CA265_RS15050 at 3792.939 kb on + strand at 3792.987 kb on - strand at 3793.027 kb on - strand at 3793.133 kb on + strand at 3793.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,789,641 - CA265_RS15030 0.90 -0.6 3,789,641 - CA265_RS15030 0.90 +0.9 3,789,663 + +0.3 3,789,740 + CA265_RS15035 0.27 +0.4 3,789,839 + CA265_RS15035 0.71 +0.4 3,789,991 + +0.8 3,790,133 + CA265_RS15040 0.16 -0.3 3,790,157 - CA265_RS15040 0.21 -0.0 3,790,160 - CA265_RS15040 0.22 -1.6 3,790,160 - CA265_RS15040 0.22 +0.2 3,790,225 + CA265_RS15040 0.36 -0.4 3,790,225 + CA265_RS15040 0.36 +0.5 3,790,227 + CA265_RS15040 0.37 +0.4 3,790,267 + CA265_RS15040 0.45 -0.9 3,790,293 + CA265_RS15040 0.51 -0.2 3,790,320 - CA265_RS15040 0.57 -0.3 3,790,361 + CA265_RS15040 0.66 +0.2 3,790,385 + CA265_RS15040 0.71 -1.0 3,790,388 - CA265_RS15040 0.72 +1.3 3,790,388 - CA265_RS15040 0.72 +0.3 3,790,400 + CA265_RS15040 0.74 -0.5 3,790,485 - -1.4 3,790,489 - -0.7 3,790,620 + -0.7 3,790,621 - +0.0 3,790,621 - +0.0 3,790,670 + -0.3 3,790,670 + -0.7 3,790,697 + -0.5 3,790,773 + CA265_RS15045 0.12 -1.0 3,790,851 + CA265_RS15045 0.17 -0.7 3,790,851 + CA265_RS15045 0.17 -1.2 3,790,851 + CA265_RS15045 0.17 +0.0 3,790,851 + CA265_RS15045 0.17 -0.4 3,790,851 + CA265_RS15045 0.17 -0.7 3,790,852 - CA265_RS15045 0.17 -1.7 3,790,852 - CA265_RS15045 0.17 -1.8 3,790,861 + CA265_RS15045 0.17 -0.5 3,790,907 - CA265_RS15045 0.20 -1.6 3,790,927 + CA265_RS15045 0.21 -3.3 3,790,937 - CA265_RS15045 0.22 -2.1 3,791,010 + CA265_RS15045 0.27 -1.7 3,791,027 + CA265_RS15045 0.28 -1.1 3,791,028 - CA265_RS15045 0.28 +0.3 3,791,028 - CA265_RS15045 0.28 -0.3 3,791,029 + CA265_RS15045 0.28 +0.2 3,791,069 - CA265_RS15045 0.30 -2.3 3,791,074 - CA265_RS15045 0.31 -1.3 3,791,175 + CA265_RS15045 0.37 -1.0 3,791,188 + CA265_RS15045 0.38 -0.8 3,791,189 - CA265_RS15045 0.38 -0.1 3,791,271 + CA265_RS15045 0.43 -1.3 3,791,272 - CA265_RS15045 0.43 -2.7 3,791,275 + CA265_RS15045 0.43 -0.0 3,791,276 - CA265_RS15045 0.43 -0.5 3,791,276 - CA265_RS15045 0.43 -0.9 3,791,287 + CA265_RS15045 0.44 +0.9 3,791,296 - CA265_RS15045 0.44 -0.7 3,791,310 + CA265_RS15045 0.45 -0.5 3,791,311 - CA265_RS15045 0.45 -1.2 3,791,350 - CA265_RS15045 0.48 -0.6 3,791,418 - CA265_RS15045 0.52 -0.9 3,791,427 + CA265_RS15045 0.53 -0.4 3,791,427 + CA265_RS15045 0.53 -1.3 3,791,428 - CA265_RS15045 0.53 -0.3 3,791,514 + CA265_RS15045 0.58 -0.3 3,791,569 + CA265_RS15045 0.61 -1.2 3,791,599 + CA265_RS15045 0.63 -1.1 3,791,914 - CA265_RS15045 0.83 -0.5 3,791,914 - CA265_RS15045 0.83 +0.7 3,791,928 + CA265_RS15045 0.84 -1.2 3,791,947 + CA265_RS15045 0.85 -0.8 3,791,962 - CA265_RS15045 0.86 -1.1 3,791,991 - CA265_RS15045 0.88 -0.2 3,792,140 - +0.2 3,792,141 + -0.8 3,792,148 - +0.9 3,792,148 - -0.8 3,792,151 + -0.1 3,792,288 + +0.2 3,792,289 - -0.6 3,792,325 + -0.3 3,792,325 + -1.7 3,792,326 - -0.4 3,792,363 + -0.3 3,792,379 - -0.1 3,792,431 + CA265_RS15050 0.15 +0.0 3,792,461 + CA265_RS15050 0.19 -0.3 3,792,534 + CA265_RS15050 0.31 -0.4 3,792,535 - CA265_RS15050 0.31 +0.4 3,792,553 + CA265_RS15050 0.34 +0.2 3,792,553 + CA265_RS15050 0.34 -0.1 3,792,553 + CA265_RS15050 0.34 -0.8 3,792,554 - CA265_RS15050 0.34 +0.1 3,792,554 - CA265_RS15050 0.34 -1.5 3,792,557 + CA265_RS15050 0.35 +0.1 3,792,558 - CA265_RS15050 0.35 -0.4 3,792,590 + CA265_RS15050 0.40 +1.5 3,792,591 - CA265_RS15050 0.40 -0.1 3,792,596 + CA265_RS15050 0.41 +1.3 3,792,596 + CA265_RS15050 0.41 +0.0 3,792,596 + CA265_RS15050 0.41 +0.1 3,792,598 + CA265_RS15050 0.41 +0.5 3,792,598 + CA265_RS15050 0.41 +0.3 3,792,598 + CA265_RS15050 0.41 -0.3 3,792,599 - CA265_RS15050 0.41 +0.2 3,792,599 - CA265_RS15050 0.41 -0.7 3,792,633 + CA265_RS15050 0.47 +0.3 3,792,675 - CA265_RS15050 0.53 -0.7 3,792,732 - CA265_RS15050 0.63 -0.7 3,792,740 + CA265_RS15050 0.64 -0.8 3,792,816 + CA265_RS15050 0.76 +0.1 3,792,838 + CA265_RS15050 0.79 +0.2 3,792,847 - CA265_RS15050 0.81 +0.1 3,792,864 - CA265_RS15050 0.84 -0.3 3,792,879 + CA265_RS15050 0.86 -0.4 3,792,939 + +0.4 3,792,987 - +0.0 3,793,027 - -0.6 3,793,133 + +0.0 3,793,134 - +0.3
Or see this region's nucleotide sequence