Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14735 and CA265_RS14740 overlap by 1 nucleotides CA265_RS14740 and CA265_RS14745 overlap by 1 nucleotides CA265_RS14745 and CA265_RS14750 overlap by 1 nucleotides
CA265_RS14735: CA265_RS14735 - hypothetical protein, at 3,712,044 to 3,713,732
_RS14735
CA265_RS14740: CA265_RS14740 - biopolymer transporter ExbB, at 3,713,732 to 3,714,436
_RS14740
CA265_RS14745: CA265_RS14745 - biopolymer transporter ExbD, at 3,714,436 to 3,714,837
_RS14745
CA265_RS14750: CA265_RS14750 - energy transducer TonB, at 3,714,837 to 3,715,676
_RS14750
Position (kb)
3713
3714
3715 Strain fitness (log2 ratio)
-2
-1
0
1 at 3712.802 kb on + strand, within CA265_RS14735 at 3712.802 kb on + strand, within CA265_RS14735 at 3712.948 kb on + strand, within CA265_RS14735 at 3713.031 kb on + strand, within CA265_RS14735 at 3713.072 kb on - strand, within CA265_RS14735 at 3713.190 kb on - strand, within CA265_RS14735 at 3713.227 kb on + strand, within CA265_RS14735 at 3713.232 kb on + strand, within CA265_RS14735 at 3713.329 kb on + strand, within CA265_RS14735 at 3713.330 kb on - strand, within CA265_RS14735 at 3713.330 kb on - strand, within CA265_RS14735 at 3713.336 kb on + strand, within CA265_RS14735 at 3713.351 kb on - strand, within CA265_RS14735 at 3713.446 kb on + strand, within CA265_RS14735 at 3713.447 kb on - strand, within CA265_RS14735 at 3713.499 kb on + strand, within CA265_RS14735 at 3713.541 kb on + strand, within CA265_RS14735 at 3713.542 kb on + strand, within CA265_RS14735 at 3713.543 kb on - strand, within CA265_RS14735 at 3713.615 kb on - strand at 3713.618 kb on + strand at 3713.618 kb on + strand at 3713.618 kb on + strand at 3713.618 kb on + strand at 3713.664 kb on - strand at 3713.920 kb on - strand, within CA265_RS14740 at 3713.920 kb on - strand, within CA265_RS14740 at 3713.920 kb on - strand, within CA265_RS14740 at 3713.976 kb on - strand, within CA265_RS14740 at 3713.976 kb on - strand, within CA265_RS14740 at 3713.978 kb on - strand, within CA265_RS14740 at 3714.025 kb on + strand, within CA265_RS14740 at 3714.149 kb on - strand, within CA265_RS14740 at 3714.154 kb on - strand, within CA265_RS14740 at 3714.215 kb on - strand, within CA265_RS14740 at 3714.217 kb on + strand, within CA265_RS14740 at 3714.279 kb on + strand, within CA265_RS14740 at 3714.280 kb on - strand, within CA265_RS14740 at 3714.281 kb on + strand, within CA265_RS14740 at 3714.313 kb on - strand, within CA265_RS14740 at 3714.336 kb on + strand, within CA265_RS14740 at 3714.336 kb on + strand, within CA265_RS14740 at 3714.337 kb on - strand, within CA265_RS14740 at 3714.337 kb on - strand, within CA265_RS14740 at 3714.337 kb on - strand, within CA265_RS14740 at 3714.337 kb on - strand, within CA265_RS14740 at 3714.379 kb on + strand at 3714.474 kb on - strand at 3714.477 kb on + strand, within CA265_RS14745 at 3714.478 kb on - strand, within CA265_RS14745 at 3714.543 kb on - strand, within CA265_RS14745 at 3714.615 kb on + strand, within CA265_RS14745 at 3714.720 kb on + strand, within CA265_RS14745 at 3714.740 kb on + strand, within CA265_RS14745 at 3714.741 kb on - strand, within CA265_RS14745 at 3714.860 kb on - strand at 3714.879 kb on + strand at 3714.977 kb on - strand, within CA265_RS14750 at 3714.989 kb on + strand, within CA265_RS14750 at 3714.998 kb on - strand, within CA265_RS14750 at 3715.038 kb on - strand, within CA265_RS14750 at 3715.038 kb on - strand, within CA265_RS14750 at 3715.043 kb on + strand, within CA265_RS14750 at 3715.187 kb on - strand, within CA265_RS14750 at 3715.263 kb on - strand, within CA265_RS14750 at 3715.415 kb on - strand, within CA265_RS14750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,712,802 + CA265_RS14735 0.45 +0.1 3,712,802 + CA265_RS14735 0.45 -1.6 3,712,948 + CA265_RS14735 0.54 +0.9 3,713,031 + CA265_RS14735 0.58 -0.9 3,713,072 - CA265_RS14735 0.61 -0.4 3,713,190 - CA265_RS14735 0.68 -0.2 3,713,227 + CA265_RS14735 0.70 +0.7 3,713,232 + CA265_RS14735 0.70 -1.4 3,713,329 + CA265_RS14735 0.76 -0.5 3,713,330 - CA265_RS14735 0.76 +0.5 3,713,330 - CA265_RS14735 0.76 +0.6 3,713,336 + CA265_RS14735 0.76 -0.0 3,713,351 - CA265_RS14735 0.77 -0.3 3,713,446 + CA265_RS14735 0.83 -0.1 3,713,447 - CA265_RS14735 0.83 -0.6 3,713,499 + CA265_RS14735 0.86 -0.0 3,713,541 + CA265_RS14735 0.89 +1.2 3,713,542 + CA265_RS14735 0.89 -0.3 3,713,543 - CA265_RS14735 0.89 -1.3 3,713,615 - -0.1 3,713,618 + -1.2 3,713,618 + -0.3 3,713,618 + -0.8 3,713,618 + -0.0 3,713,664 - +0.4 3,713,920 - CA265_RS14740 0.27 -0.7 3,713,920 - CA265_RS14740 0.27 -0.7 3,713,920 - CA265_RS14740 0.27 -0.6 3,713,976 - CA265_RS14740 0.35 +0.3 3,713,976 - CA265_RS14740 0.35 +0.3 3,713,978 - CA265_RS14740 0.35 -0.2 3,714,025 + CA265_RS14740 0.42 -1.0 3,714,149 - CA265_RS14740 0.59 -0.7 3,714,154 - CA265_RS14740 0.60 -1.6 3,714,215 - CA265_RS14740 0.69 -0.3 3,714,217 + CA265_RS14740 0.69 -0.2 3,714,279 + CA265_RS14740 0.78 -0.4 3,714,280 - CA265_RS14740 0.78 +0.2 3,714,281 + CA265_RS14740 0.78 +0.9 3,714,313 - CA265_RS14740 0.82 -0.4 3,714,336 + CA265_RS14740 0.86 -1.4 3,714,336 + CA265_RS14740 0.86 +0.6 3,714,337 - CA265_RS14740 0.86 -0.8 3,714,337 - CA265_RS14740 0.86 -0.8 3,714,337 - CA265_RS14740 0.86 -0.7 3,714,337 - CA265_RS14740 0.86 -0.0 3,714,379 + -2.1 3,714,474 - -0.2 3,714,477 + CA265_RS14745 0.10 -0.8 3,714,478 - CA265_RS14745 0.10 -0.9 3,714,543 - CA265_RS14745 0.27 +0.1 3,714,615 + CA265_RS14745 0.45 +0.3 3,714,720 + CA265_RS14745 0.71 -0.1 3,714,740 + CA265_RS14745 0.76 -1.2 3,714,741 - CA265_RS14745 0.76 -0.3 3,714,860 - +0.5 3,714,879 + -1.0 3,714,977 - CA265_RS14750 0.17 -0.2 3,714,989 + CA265_RS14750 0.18 -1.5 3,714,998 - CA265_RS14750 0.19 +0.3 3,715,038 - CA265_RS14750 0.24 -0.1 3,715,038 - CA265_RS14750 0.24 -0.1 3,715,043 + CA265_RS14750 0.25 +0.7 3,715,187 - CA265_RS14750 0.42 -2.0 3,715,263 - CA265_RS14750 0.51 -1.2 3,715,415 - CA265_RS14750 0.69 -0.7
Or see this region's nucleotide sequence