Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13955 and CA265_RS13960 overlap by 4 nucleotides
CA265_RS13955: CA265_RS13955 - patatin, at 3,512,149 to 3,514,440
_RS13955
CA265_RS13960: CA265_RS13960 - hypothetical protein, at 3,514,437 to 3,514,967
_RS13960
Position (kb)
3514
3515 Strain fitness (log2 ratio)
-1
0
1
2 at 3513.504 kb on - strand, within CA265_RS13955 at 3513.527 kb on + strand, within CA265_RS13955 at 3513.550 kb on + strand, within CA265_RS13955 at 3513.550 kb on + strand, within CA265_RS13955 at 3513.551 kb on - strand, within CA265_RS13955 at 3513.553 kb on - strand, within CA265_RS13955 at 3513.573 kb on + strand, within CA265_RS13955 at 3513.573 kb on + strand, within CA265_RS13955 at 3513.573 kb on + strand, within CA265_RS13955 at 3513.573 kb on + strand, within CA265_RS13955 at 3513.574 kb on - strand, within CA265_RS13955 at 3513.630 kb on + strand, within CA265_RS13955 at 3513.631 kb on - strand, within CA265_RS13955 at 3513.691 kb on - strand, within CA265_RS13955 at 3513.697 kb on - strand, within CA265_RS13955 at 3513.730 kb on - strand, within CA265_RS13955 at 3513.753 kb on - strand, within CA265_RS13955 at 3513.768 kb on + strand, within CA265_RS13955 at 3513.769 kb on - strand, within CA265_RS13955 at 3513.788 kb on + strand, within CA265_RS13955 at 3513.823 kb on + strand, within CA265_RS13955 at 3513.824 kb on - strand, within CA265_RS13955 at 3513.862 kb on + strand, within CA265_RS13955 at 3513.867 kb on + strand, within CA265_RS13955 at 3513.868 kb on - strand, within CA265_RS13955 at 3513.868 kb on - strand, within CA265_RS13955 at 3513.868 kb on - strand, within CA265_RS13955 at 3514.023 kb on + strand, within CA265_RS13955 at 3514.056 kb on + strand, within CA265_RS13955 at 3514.146 kb on + strand, within CA265_RS13955 at 3514.147 kb on - strand, within CA265_RS13955 at 3514.167 kb on - strand, within CA265_RS13955 at 3514.178 kb on - strand, within CA265_RS13955 at 3514.179 kb on + strand, within CA265_RS13955 at 3514.221 kb on - strand at 3514.234 kb on - strand at 3514.266 kb on - strand at 3514.321 kb on + strand at 3514.356 kb on + strand at 3514.361 kb on + strand at 3514.364 kb on - strand at 3514.391 kb on + strand at 3514.394 kb on + strand at 3514.408 kb on + strand at 3514.451 kb on - strand at 3514.471 kb on + strand at 3514.547 kb on + strand, within CA265_RS13960 at 3514.560 kb on - strand, within CA265_RS13960 at 3514.611 kb on - strand, within CA265_RS13960 at 3514.632 kb on - strand, within CA265_RS13960 at 3514.685 kb on + strand, within CA265_RS13960 at 3514.700 kb on - strand, within CA265_RS13960 at 3514.703 kb on + strand, within CA265_RS13960 at 3514.703 kb on + strand, within CA265_RS13960 at 3514.706 kb on + strand, within CA265_RS13960 at 3514.707 kb on - strand, within CA265_RS13960 at 3514.746 kb on + strand, within CA265_RS13960 at 3514.746 kb on + strand, within CA265_RS13960 at 3514.747 kb on - strand, within CA265_RS13960 at 3514.747 kb on - strand, within CA265_RS13960 at 3514.753 kb on - strand, within CA265_RS13960 at 3514.791 kb on - strand, within CA265_RS13960 at 3514.904 kb on + strand, within CA265_RS13960 at 3514.923 kb on - strand at 3514.971 kb on - strand at 3515.073 kb on + strand at 3515.222 kb on + strand at 3515.222 kb on + strand at 3515.225 kb on + strand at 3515.235 kb on - strand at 3515.245 kb on - strand at 3515.271 kb on + strand at 3515.373 kb on + strand at 3515.484 kb on - strand at 3515.578 kb on + strand at 3515.585 kb on - strand at 3515.672 kb on - strand at 3515.737 kb on + strand at 3515.737 kb on + strand at 3515.737 kb on + strand at 3515.738 kb on - strand at 3515.738 kb on - strand at 3515.790 kb on + strand at 3515.802 kb on + strand at 3515.856 kb on - strand at 3515.870 kb on - strand at 3515.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,513,504 - CA265_RS13955 0.59 +0.1 3,513,527 + CA265_RS13955 0.60 -0.1 3,513,550 + CA265_RS13955 0.61 +0.0 3,513,550 + CA265_RS13955 0.61 +0.4 3,513,551 - CA265_RS13955 0.61 -0.1 3,513,553 - CA265_RS13955 0.61 -0.2 3,513,573 + CA265_RS13955 0.62 -0.8 3,513,573 + CA265_RS13955 0.62 +0.1 3,513,573 + CA265_RS13955 0.62 -0.4 3,513,573 + CA265_RS13955 0.62 -0.0 3,513,574 - CA265_RS13955 0.62 -0.1 3,513,630 + CA265_RS13955 0.65 -0.2 3,513,631 - CA265_RS13955 0.65 +0.2 3,513,691 - CA265_RS13955 0.67 +0.0 3,513,697 - CA265_RS13955 0.68 -0.4 3,513,730 - CA265_RS13955 0.69 +0.5 3,513,753 - CA265_RS13955 0.70 -0.2 3,513,768 + CA265_RS13955 0.71 -0.5 3,513,769 - CA265_RS13955 0.71 -0.3 3,513,788 + CA265_RS13955 0.72 -0.4 3,513,823 + CA265_RS13955 0.73 +1.2 3,513,824 - CA265_RS13955 0.73 -0.1 3,513,862 + CA265_RS13955 0.75 +0.3 3,513,867 + CA265_RS13955 0.75 -0.2 3,513,868 - CA265_RS13955 0.75 -0.3 3,513,868 - CA265_RS13955 0.75 +0.5 3,513,868 - CA265_RS13955 0.75 +0.2 3,514,023 + CA265_RS13955 0.82 +0.9 3,514,056 + CA265_RS13955 0.83 +0.2 3,514,146 + CA265_RS13955 0.87 -0.3 3,514,147 - CA265_RS13955 0.87 +0.6 3,514,167 - CA265_RS13955 0.88 -0.2 3,514,178 - CA265_RS13955 0.89 -1.3 3,514,179 + CA265_RS13955 0.89 +0.3 3,514,221 - -0.1 3,514,234 - -0.3 3,514,266 - -1.6 3,514,321 + +0.7 3,514,356 + +0.0 3,514,361 + +0.7 3,514,364 - +2.2 3,514,391 + -0.4 3,514,394 + -0.6 3,514,408 + +0.5 3,514,451 - -0.9 3,514,471 + -0.5 3,514,547 + CA265_RS13960 0.21 -1.0 3,514,560 - CA265_RS13960 0.23 -1.0 3,514,611 - CA265_RS13960 0.33 -1.3 3,514,632 - CA265_RS13960 0.37 -0.6 3,514,685 + CA265_RS13960 0.47 -1.0 3,514,700 - CA265_RS13960 0.50 -0.9 3,514,703 + CA265_RS13960 0.50 -0.6 3,514,703 + CA265_RS13960 0.50 -1.2 3,514,706 + CA265_RS13960 0.51 +0.6 3,514,707 - CA265_RS13960 0.51 -0.7 3,514,746 + CA265_RS13960 0.58 -0.4 3,514,746 + CA265_RS13960 0.58 -0.5 3,514,747 - CA265_RS13960 0.58 +0.1 3,514,747 - CA265_RS13960 0.58 -0.6 3,514,753 - CA265_RS13960 0.60 -0.7 3,514,791 - CA265_RS13960 0.67 -1.1 3,514,904 + CA265_RS13960 0.88 +0.1 3,514,923 - -0.3 3,514,971 - -1.1 3,515,073 + +1.2 3,515,222 + -0.5 3,515,222 + -0.2 3,515,225 + -0.1 3,515,235 - -1.6 3,515,245 - -0.0 3,515,271 + +0.4 3,515,373 + -1.3 3,515,484 - +0.3 3,515,578 + +0.3 3,515,585 - +0.2 3,515,672 - -0.5 3,515,737 + +0.2 3,515,737 + -0.3 3,515,737 + -0.4 3,515,738 - -0.1 3,515,738 - -0.7 3,515,790 + -0.7 3,515,802 + +0.3 3,515,856 - -0.4 3,515,870 - +0.0 3,515,870 - -0.3
Or see this region's nucleotide sequence