Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12935 and CA265_RS12940 are separated by 553 nucleotides CA265_RS12940 and CA265_RS12945 are separated by 302 nucleotides
CA265_RS12935: CA265_RS12935 - arabinan endo-1,5-alpha-L-arabinosidase, at 3,221,040 to 3,222,020
_RS12935
CA265_RS12940: CA265_RS12940 - peptidase dimerization domain protein, at 3,222,574 to 3,223,944
_RS12940
CA265_RS12945: CA265_RS12945 - hypothetical protein, at 3,224,247 to 3,225,221
_RS12945
Position (kb)
3222
3223
3224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3221.772 kb on - strand, within CA265_RS12935 at 3221.781 kb on - strand, within CA265_RS12935 at 3221.846 kb on + strand, within CA265_RS12935 at 3221.870 kb on - strand, within CA265_RS12935 at 3221.883 kb on - strand, within CA265_RS12935 at 3221.883 kb on - strand, within CA265_RS12935 at 3221.994 kb on + strand at 3222.010 kb on - strand at 3222.010 kb on - strand at 3222.091 kb on - strand at 3222.147 kb on + strand at 3222.148 kb on - strand at 3222.187 kb on + strand at 3222.279 kb on + strand at 3222.282 kb on - strand at 3222.284 kb on + strand at 3222.309 kb on + strand at 3222.359 kb on - strand at 3222.394 kb on + strand at 3222.397 kb on - strand at 3222.575 kb on + strand at 3222.575 kb on + strand at 3222.608 kb on - strand at 3222.631 kb on - strand at 3222.635 kb on + strand at 3222.721 kb on + strand, within CA265_RS12940 at 3222.722 kb on - strand, within CA265_RS12940 at 3222.722 kb on - strand, within CA265_RS12940 at 3222.887 kb on - strand, within CA265_RS12940 at 3223.025 kb on + strand, within CA265_RS12940 at 3223.221 kb on + strand, within CA265_RS12940 at 3223.221 kb on + strand at 3223.222 kb on - strand, within CA265_RS12940 at 3223.349 kb on + strand, within CA265_RS12940 at 3223.562 kb on - strand, within CA265_RS12940 at 3223.569 kb on + strand, within CA265_RS12940 at 3223.648 kb on - strand, within CA265_RS12940 at 3223.648 kb on - strand, within CA265_RS12940 at 3223.695 kb on - strand, within CA265_RS12940 at 3223.695 kb on - strand, within CA265_RS12940 at 3223.747 kb on - strand, within CA265_RS12940 at 3223.814 kb on + strand at 3223.815 kb on - strand at 3223.815 kb on - strand at 3224.010 kb on + strand at 3224.054 kb on - strand at 3224.054 kb on - strand at 3224.093 kb on + strand at 3224.180 kb on + strand at 3224.338 kb on + strand at 3224.339 kb on - strand at 3224.362 kb on - strand, within CA265_RS12945 at 3224.362 kb on - strand, within CA265_RS12945 at 3224.380 kb on + strand, within CA265_RS12945 at 3224.470 kb on - strand, within CA265_RS12945 at 3224.493 kb on - strand, within CA265_RS12945 at 3224.504 kb on + strand, within CA265_RS12945 at 3224.506 kb on + strand, within CA265_RS12945 at 3224.562 kb on - strand, within CA265_RS12945 at 3224.595 kb on - strand, within CA265_RS12945 at 3224.630 kb on + strand, within CA265_RS12945 at 3224.631 kb on - strand, within CA265_RS12945 at 3224.706 kb on - strand, within CA265_RS12945 at 3224.753 kb on - strand, within CA265_RS12945 at 3224.753 kb on - strand, within CA265_RS12945 at 3224.756 kb on + strand, within CA265_RS12945 at 3224.756 kb on + strand, within CA265_RS12945 at 3224.795 kb on - strand, within CA265_RS12945 at 3224.807 kb on + strand, within CA265_RS12945 at 3224.807 kb on + strand, within CA265_RS12945 at 3224.860 kb on - strand, within CA265_RS12945 at 3224.860 kb on - strand, within CA265_RS12945 at 3224.894 kb on + strand, within CA265_RS12945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,221,772 - CA265_RS12935 0.75 -0.9 3,221,781 - CA265_RS12935 0.76 +0.8 3,221,846 + CA265_RS12935 0.82 -0.3 3,221,870 - CA265_RS12935 0.85 -0.6 3,221,883 - CA265_RS12935 0.86 +0.4 3,221,883 - CA265_RS12935 0.86 -0.2 3,221,994 + -0.4 3,222,010 - -0.0 3,222,010 - -0.1 3,222,091 - -0.0 3,222,147 + -0.2 3,222,148 - +0.7 3,222,187 + +0.3 3,222,279 + -0.2 3,222,282 - -0.2 3,222,284 + -0.5 3,222,309 + +0.2 3,222,359 - +0.6 3,222,394 + -0.4 3,222,397 - +0.6 3,222,575 + -0.2 3,222,575 + +0.3 3,222,608 - -0.2 3,222,631 - -1.2 3,222,635 + -1.0 3,222,721 + CA265_RS12940 0.11 -0.8 3,222,722 - CA265_RS12940 0.11 -1.1 3,222,722 - CA265_RS12940 0.11 -2.6 3,222,887 - CA265_RS12940 0.23 -1.2 3,223,025 + CA265_RS12940 0.33 -0.5 3,223,221 + CA265_RS12940 0.47 -0.6 3,223,221 + +0.8 3,223,222 - CA265_RS12940 0.47 -1.5 3,223,349 + CA265_RS12940 0.57 -1.6 3,223,562 - CA265_RS12940 0.72 +0.8 3,223,569 + CA265_RS12940 0.73 -0.2 3,223,648 - CA265_RS12940 0.78 +1.8 3,223,648 - CA265_RS12940 0.78 -1.3 3,223,695 - CA265_RS12940 0.82 +0.1 3,223,695 - CA265_RS12940 0.82 -0.1 3,223,747 - CA265_RS12940 0.86 +1.2 3,223,814 + -1.6 3,223,815 - -1.0 3,223,815 - -1.5 3,224,010 + -0.2 3,224,054 - -0.4 3,224,054 - +0.1 3,224,093 + +0.0 3,224,180 + -0.0 3,224,338 + -0.2 3,224,339 - -0.7 3,224,362 - CA265_RS12945 0.12 -0.2 3,224,362 - CA265_RS12945 0.12 +0.8 3,224,380 + CA265_RS12945 0.14 +0.9 3,224,470 - CA265_RS12945 0.23 +0.5 3,224,493 - CA265_RS12945 0.25 +0.5 3,224,504 + CA265_RS12945 0.26 +1.7 3,224,506 + CA265_RS12945 0.27 +0.2 3,224,562 - CA265_RS12945 0.32 +0.5 3,224,595 - CA265_RS12945 0.36 -1.8 3,224,630 + CA265_RS12945 0.39 +0.3 3,224,631 - CA265_RS12945 0.39 +0.4 3,224,706 - CA265_RS12945 0.47 +0.5 3,224,753 - CA265_RS12945 0.52 -0.2 3,224,753 - CA265_RS12945 0.52 +1.5 3,224,756 + CA265_RS12945 0.52 +0.7 3,224,756 + CA265_RS12945 0.52 -0.2 3,224,795 - CA265_RS12945 0.56 -0.0 3,224,807 + CA265_RS12945 0.57 -0.3 3,224,807 + CA265_RS12945 0.57 -0.0 3,224,860 - CA265_RS12945 0.63 +0.5 3,224,860 - CA265_RS12945 0.63 +0.1 3,224,894 + CA265_RS12945 0.66 +0.3
Or see this region's nucleotide sequence