Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12730 and CA265_RS12735 are separated by 261 nucleotides CA265_RS12735 and CA265_RS12740 overlap by 11 nucleotides CA265_RS12740 and CA265_RS12745 are separated by 134 nucleotides
CA265_RS12730: CA265_RS12730 - pantetheine-phosphate adenylyltransferase, at 3,173,217 to 3,173,678
_RS12730
CA265_RS12735: CA265_RS12735 - RNA polymerase subunit sigma-70, at 3,173,940 to 3,174,443
_RS12735
CA265_RS12740: CA265_RS12740 - hypothetical protein, at 3,174,433 to 3,175,038
_RS12740
CA265_RS12745: CA265_RS12745 - NUDIX hydrolase, at 3,175,173 to 3,175,784
_RS12745
Position (kb)
3174
3175
3176 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3173.748 kb on - strand at 3173.783 kb on + strand at 3173.784 kb on - strand at 3173.820 kb on - strand at 3173.932 kb on + strand at 3173.932 kb on + strand at 3173.937 kb on + strand at 3173.996 kb on - strand, within CA265_RS12735 at 3173.999 kb on + strand, within CA265_RS12735 at 3174.081 kb on + strand, within CA265_RS12735 at 3174.099 kb on - strand, within CA265_RS12735 at 3174.148 kb on + strand, within CA265_RS12735 at 3174.148 kb on + strand, within CA265_RS12735 at 3174.148 kb on + strand, within CA265_RS12735 at 3174.149 kb on - strand, within CA265_RS12735 at 3174.153 kb on + strand, within CA265_RS12735 at 3174.153 kb on + strand, within CA265_RS12735 at 3174.182 kb on + strand, within CA265_RS12735 at 3174.182 kb on + strand, within CA265_RS12735 at 3174.183 kb on - strand, within CA265_RS12735 at 3174.211 kb on + strand, within CA265_RS12735 at 3174.255 kb on + strand, within CA265_RS12735 at 3174.256 kb on - strand, within CA265_RS12735 at 3174.261 kb on + strand, within CA265_RS12735 at 3174.304 kb on + strand, within CA265_RS12735 at 3174.418 kb on + strand at 3174.634 kb on - strand, within CA265_RS12740 at 3174.811 kb on + strand, within CA265_RS12740 at 3174.813 kb on + strand, within CA265_RS12740 at 3174.911 kb on - strand, within CA265_RS12740 at 3174.918 kb on - strand, within CA265_RS12740 at 3174.954 kb on - strand, within CA265_RS12740 at 3174.978 kb on - strand at 3175.060 kb on - strand at 3175.071 kb on + strand at 3175.072 kb on - strand at 3175.072 kb on - strand at 3175.099 kb on - strand at 3175.119 kb on + strand at 3175.174 kb on + strand at 3175.175 kb on - strand at 3175.175 kb on - strand at 3175.175 kb on - strand at 3175.176 kb on + strand at 3175.177 kb on - strand at 3175.205 kb on + strand at 3175.205 kb on + strand at 3175.206 kb on - strand at 3175.206 kb on - strand at 3175.206 kb on - strand at 3175.226 kb on + strand at 3175.227 kb on - strand at 3175.356 kb on - strand, within CA265_RS12745 at 3175.407 kb on - strand, within CA265_RS12745 at 3175.517 kb on + strand, within CA265_RS12745 at 3175.525 kb on + strand, within CA265_RS12745 at 3175.526 kb on - strand, within CA265_RS12745 at 3175.568 kb on + strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.629 kb on - strand, within CA265_RS12745 at 3175.762 kb on + strand at 3175.802 kb on - strand at 3175.831 kb on + strand at 3175.832 kb on - strand at 3175.875 kb on + strand at 3175.876 kb on - strand at 3175.876 kb on - strand at 3175.877 kb on + strand at 3175.879 kb on + strand at 3175.879 kb on + strand at 3175.901 kb on - strand at 3175.921 kb on + strand at 3175.922 kb on - strand at 3175.927 kb on + strand at 3175.931 kb on - strand at 3175.950 kb on + strand at 3175.958 kb on + strand at 3175.964 kb on + strand at 3175.968 kb on + strand at 3176.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,173,748 - +0.1 3,173,783 + +0.8 3,173,784 - +0.1 3,173,820 - +0.3 3,173,932 + +0.3 3,173,932 + -0.1 3,173,937 + -0.7 3,173,996 - CA265_RS12735 0.11 +0.0 3,173,999 + CA265_RS12735 0.12 +0.2 3,174,081 + CA265_RS12735 0.28 -1.0 3,174,099 - CA265_RS12735 0.32 +0.7 3,174,148 + CA265_RS12735 0.41 +0.9 3,174,148 + CA265_RS12735 0.41 +0.5 3,174,148 + CA265_RS12735 0.41 +0.1 3,174,149 - CA265_RS12735 0.41 -0.2 3,174,153 + CA265_RS12735 0.42 -0.1 3,174,153 + CA265_RS12735 0.42 -0.6 3,174,182 + CA265_RS12735 0.48 +0.2 3,174,182 + CA265_RS12735 0.48 -0.0 3,174,183 - CA265_RS12735 0.48 -0.8 3,174,211 + CA265_RS12735 0.54 -0.8 3,174,255 + CA265_RS12735 0.62 +0.5 3,174,256 - CA265_RS12735 0.63 -0.1 3,174,261 + CA265_RS12735 0.64 +0.3 3,174,304 + CA265_RS12735 0.72 +0.7 3,174,418 + +0.6 3,174,634 - CA265_RS12740 0.33 -1.5 3,174,811 + CA265_RS12740 0.62 -2.0 3,174,813 + CA265_RS12740 0.63 -0.8 3,174,911 - CA265_RS12740 0.79 -2.3 3,174,918 - CA265_RS12740 0.80 -1.6 3,174,954 - CA265_RS12740 0.86 -1.4 3,174,978 - +0.6 3,175,060 - -0.4 3,175,071 + +0.2 3,175,072 - +0.3 3,175,072 - -1.3 3,175,099 - +0.2 3,175,119 + -1.0 3,175,174 + +0.3 3,175,175 - +0.3 3,175,175 - +0.3 3,175,175 - +0.2 3,175,176 + -0.3 3,175,177 - +0.3 3,175,205 + +0.4 3,175,205 + -0.2 3,175,206 - +0.1 3,175,206 - +0.0 3,175,206 - -0.5 3,175,226 + +0.1 3,175,227 - -0.4 3,175,356 - CA265_RS12745 0.30 -0.0 3,175,407 - CA265_RS12745 0.38 -0.7 3,175,517 + CA265_RS12745 0.56 -0.3 3,175,525 + CA265_RS12745 0.58 -0.6 3,175,526 - CA265_RS12745 0.58 -0.5 3,175,568 + CA265_RS12745 0.65 +0.6 3,175,575 - CA265_RS12745 0.66 +0.0 3,175,575 - CA265_RS12745 0.66 +0.3 3,175,575 - CA265_RS12745 0.66 +0.0 3,175,629 - CA265_RS12745 0.75 +0.7 3,175,762 + +0.6 3,175,802 - -0.3 3,175,831 + -0.0 3,175,832 - -0.9 3,175,875 + -0.2 3,175,876 - +1.8 3,175,876 - -0.8 3,175,877 + +0.4 3,175,879 + +0.4 3,175,879 + +0.3 3,175,901 - +0.1 3,175,921 + +0.9 3,175,922 - -0.2 3,175,927 + -0.6 3,175,931 - -0.8 3,175,950 + +0.8 3,175,958 + -1.5 3,175,964 + -1.0 3,175,968 + +0.4 3,176,035 - -0.7
Or see this region's nucleotide sequence