Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11665 and CA265_RS11670 are separated by 170 nucleotides CA265_RS11670 and CA265_RS11675 are separated by 62 nucleotides
CA265_RS11665: CA265_RS11665 - transketolase, at 2,892,392 to 2,894,812
_RS11665
CA265_RS11670: CA265_RS11670 - hypothetical protein, at 2,894,983 to 2,895,375
_RS11670
CA265_RS11675: CA265_RS11675 - aspartate aminotransferase, at 2,895,438 to 2,896,637
_RS11675
Position (kb)
2894
2895
2896 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2894.886 kb on - strand at 2894.928 kb on + strand at 2894.940 kb on - strand at 2894.969 kb on + strand at 2894.979 kb on + strand at 2894.979 kb on + strand at 2894.999 kb on + strand at 2895.024 kb on - strand, within CA265_RS11670 at 2895.035 kb on + strand, within CA265_RS11670 at 2895.035 kb on + strand, within CA265_RS11670 at 2895.035 kb on + strand, within CA265_RS11670 at 2895.048 kb on + strand, within CA265_RS11670 at 2895.121 kb on + strand, within CA265_RS11670 at 2895.134 kb on - strand, within CA265_RS11670 at 2895.163 kb on - strand, within CA265_RS11670 at 2895.192 kb on + strand, within CA265_RS11670 at 2895.193 kb on - strand, within CA265_RS11670 at 2895.193 kb on - strand, within CA265_RS11670 at 2895.198 kb on - strand, within CA265_RS11670 at 2895.201 kb on + strand, within CA265_RS11670 at 2895.205 kb on + strand, within CA265_RS11670 at 2895.244 kb on - strand, within CA265_RS11670 at 2895.244 kb on - strand, within CA265_RS11670 at 2895.259 kb on - strand, within CA265_RS11670 at 2895.279 kb on + strand, within CA265_RS11670 at 2895.327 kb on + strand, within CA265_RS11670 at 2895.392 kb on - strand at 2895.549 kb on - strand at 2895.565 kb on + strand, within CA265_RS11675 at 2895.586 kb on + strand, within CA265_RS11675 at 2895.626 kb on + strand, within CA265_RS11675 at 2895.676 kb on - strand, within CA265_RS11675 at 2895.680 kb on + strand at 2895.691 kb on - strand, within CA265_RS11675 at 2895.696 kb on - strand, within CA265_RS11675 at 2895.698 kb on + strand, within CA265_RS11675 at 2895.698 kb on + strand, within CA265_RS11675 at 2895.699 kb on - strand, within CA265_RS11675 at 2895.813 kb on + strand, within CA265_RS11675 at 2895.813 kb on + strand, within CA265_RS11675 at 2895.992 kb on - strand, within CA265_RS11675 at 2896.000 kb on + strand, within CA265_RS11675 at 2896.016 kb on + strand, within CA265_RS11675 at 2896.021 kb on - strand, within CA265_RS11675 at 2896.100 kb on - strand, within CA265_RS11675 at 2896.109 kb on - strand, within CA265_RS11675 at 2896.180 kb on + strand, within CA265_RS11675 at 2896.181 kb on - strand, within CA265_RS11675 at 2896.237 kb on + strand, within CA265_RS11675 at 2896.279 kb on + strand, within CA265_RS11675 at 2896.279 kb on + strand, within CA265_RS11675 at 2896.297 kb on - strand, within CA265_RS11675 at 2896.322 kb on + strand, within CA265_RS11675 at 2896.343 kb on - strand, within CA265_RS11675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,894,886 - -1.7 2,894,928 + -0.6 2,894,940 - -0.4 2,894,969 + -0.6 2,894,979 + -0.5 2,894,979 + +0.6 2,894,999 + +0.0 2,895,024 - CA265_RS11670 0.10 -1.3 2,895,035 + CA265_RS11670 0.13 -0.2 2,895,035 + CA265_RS11670 0.13 -4.2 2,895,035 + CA265_RS11670 0.13 -0.9 2,895,048 + CA265_RS11670 0.17 -0.3 2,895,121 + CA265_RS11670 0.35 -1.2 2,895,134 - CA265_RS11670 0.38 -1.5 2,895,163 - CA265_RS11670 0.46 -0.4 2,895,192 + CA265_RS11670 0.53 -4.2 2,895,193 - CA265_RS11670 0.53 -0.7 2,895,193 - CA265_RS11670 0.53 -0.7 2,895,198 - CA265_RS11670 0.55 -1.4 2,895,201 + CA265_RS11670 0.55 -0.4 2,895,205 + CA265_RS11670 0.56 -1.5 2,895,244 - CA265_RS11670 0.66 -0.8 2,895,244 - CA265_RS11670 0.66 -0.8 2,895,259 - CA265_RS11670 0.70 -0.9 2,895,279 + CA265_RS11670 0.75 -1.4 2,895,327 + CA265_RS11670 0.88 -0.2 2,895,392 - +0.5 2,895,549 - -0.2 2,895,565 + CA265_RS11675 0.11 +0.0 2,895,586 + CA265_RS11675 0.12 -0.3 2,895,626 + CA265_RS11675 0.16 -0.9 2,895,676 - CA265_RS11675 0.20 -0.7 2,895,680 + -0.1 2,895,691 - CA265_RS11675 0.21 -0.0 2,895,696 - CA265_RS11675 0.21 -0.5 2,895,698 + CA265_RS11675 0.22 -0.6 2,895,698 + CA265_RS11675 0.22 -0.0 2,895,699 - CA265_RS11675 0.22 -0.2 2,895,813 + CA265_RS11675 0.31 +0.2 2,895,813 + CA265_RS11675 0.31 -0.6 2,895,992 - CA265_RS11675 0.46 -0.0 2,896,000 + CA265_RS11675 0.47 -0.1 2,896,016 + CA265_RS11675 0.48 -0.2 2,896,021 - CA265_RS11675 0.49 +0.3 2,896,100 - CA265_RS11675 0.55 -0.3 2,896,109 - CA265_RS11675 0.56 +0.2 2,896,180 + CA265_RS11675 0.62 -0.4 2,896,181 - CA265_RS11675 0.62 +0.2 2,896,237 + CA265_RS11675 0.67 -0.2 2,896,279 + CA265_RS11675 0.70 -0.5 2,896,279 + CA265_RS11675 0.70 +0.1 2,896,297 - CA265_RS11675 0.72 -0.8 2,896,322 + CA265_RS11675 0.74 -0.4 2,896,343 - CA265_RS11675 0.75 -0.8
Or see this region's nucleotide sequence