Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10915 and CA265_RS10920 are separated by 784 nucleotides CA265_RS10920 and CA265_RS10925 are separated by 80 nucleotides CA265_RS10925 and CA265_RS10930 are separated by 92 nucleotides
CA265_RS10915: CA265_RS10915 - hypothetical protein, at 2,686,065 to 2,686,544
_RS10915
CA265_RS10920: CA265_RS10920 - SsrA-binding protein, at 2,687,329 to 2,687,778
_RS10920
CA265_RS10925: CA265_RS10925 - protein-L-isoaspartate O-methyltransferase, at 2,687,859 to 2,688,515
_RS10925
CA265_RS10930: CA265_RS10930 - primosomal protein N', at 2,688,608 to 2,691,088
_RS10930
Position (kb)
2687
2688 Strain fitness (log2 ratio)
-2
-1
0
1 at 2686.450 kb on + strand, within CA265_RS10915 at 2686.594 kb on + strand at 2686.596 kb on + strand at 2686.597 kb on - strand at 2686.817 kb on + strand at 2686.818 kb on - strand at 2686.835 kb on + strand at 2686.840 kb on - strand at 2686.973 kb on - strand at 2687.093 kb on - strand at 2687.093 kb on - strand at 2687.205 kb on + strand at 2687.205 kb on + strand at 2687.207 kb on + strand at 2687.208 kb on - strand at 2687.229 kb on + strand at 2687.283 kb on + strand at 2687.284 kb on - strand at 2687.285 kb on + strand at 2687.295 kb on + strand at 2687.297 kb on + strand at 2687.312 kb on + strand at 2687.392 kb on + strand, within CA265_RS10920 at 2687.478 kb on + strand, within CA265_RS10920 at 2687.493 kb on - strand, within CA265_RS10920 at 2687.494 kb on + strand, within CA265_RS10920 at 2687.573 kb on + strand, within CA265_RS10920 at 2687.627 kb on - strand, within CA265_RS10920 at 2687.730 kb on + strand, within CA265_RS10920 at 2687.743 kb on - strand at 2687.857 kb on + strand at 2687.857 kb on + strand at 2687.858 kb on - strand at 2688.409 kb on - strand, within CA265_RS10925 at 2688.548 kb on + strand at 2688.610 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,686,450 + CA265_RS10915 0.80 +0.0 2,686,594 + -0.5 2,686,596 + +0.4 2,686,597 - +0.1 2,686,817 + -0.4 2,686,818 - -0.1 2,686,835 + +0.1 2,686,840 - +0.7 2,686,973 - +0.4 2,687,093 - +0.5 2,687,093 - -0.1 2,687,205 + +0.5 2,687,205 + -0.4 2,687,207 + -0.3 2,687,208 - -0.5 2,687,229 + +0.2 2,687,283 + -1.3 2,687,284 - -1.0 2,687,285 + +0.0 2,687,295 + -0.2 2,687,297 + +0.2 2,687,312 + +0.1 2,687,392 + CA265_RS10920 0.14 -0.2 2,687,478 + CA265_RS10920 0.33 -1.7 2,687,493 - CA265_RS10920 0.36 -2.7 2,687,494 + CA265_RS10920 0.37 -1.7 2,687,573 + CA265_RS10920 0.54 -2.0 2,687,627 - CA265_RS10920 0.66 -0.2 2,687,730 + CA265_RS10920 0.89 -2.4 2,687,743 - -0.3 2,687,857 + -0.0 2,687,857 + +0.2 2,687,858 - +0.4 2,688,409 - CA265_RS10925 0.84 -0.9 2,688,548 + -0.7 2,688,610 - +0.7
Or see this region's nucleotide sequence