Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10535 and CA265_RS10540 are separated by 328 nucleotides CA265_RS10540 and CA265_RS10545 are separated by 940 nucleotides
CA265_RS10535: CA265_RS10535 - isoprenyl synthetase, at 2,608,785 to 2,609,759
_RS10535
CA265_RS10540: CA265_RS10540 - dinitrogenase iron-molybdenum cofactor biosynthesis protein, at 2,610,088 to 2,610,195
_RS10540
CA265_RS10545: CA265_RS10545 - hypothetical protein, at 2,611,136 to 2,612,116
_RS10545
Position (kb)
2610
2611 Strain fitness (log2 ratio)
-2
-1
0
1 at 2609.763 kb on - strand at 2609.832 kb on + strand at 2609.892 kb on + strand at 2609.905 kb on - strand at 2609.905 kb on - strand at 2610.017 kb on + strand at 2610.017 kb on + strand at 2610.126 kb on + strand, within CA265_RS10540 at 2610.127 kb on - strand, within CA265_RS10540 at 2610.216 kb on + strand at 2610.228 kb on + strand at 2610.258 kb on + strand at 2610.266 kb on + strand at 2610.266 kb on + strand at 2610.268 kb on + strand at 2610.268 kb on + strand at 2610.296 kb on - strand at 2610.394 kb on + strand at 2610.395 kb on - strand at 2610.412 kb on - strand at 2610.436 kb on - strand at 2610.481 kb on + strand at 2610.589 kb on - strand at 2610.592 kb on + strand at 2610.656 kb on + strand at 2610.710 kb on + strand at 2610.787 kb on + strand at 2610.787 kb on + strand at 2610.817 kb on + strand at 2610.822 kb on + strand at 2610.847 kb on + strand at 2610.868 kb on + strand at 2610.877 kb on - strand at 2610.884 kb on + strand at 2610.885 kb on - strand at 2610.992 kb on + strand at 2610.992 kb on + strand at 2610.992 kb on + strand at 2610.992 kb on + strand at 2610.993 kb on - strand at 2611.025 kb on + strand at 2611.053 kb on - strand at 2611.072 kb on - strand at 2611.115 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,609,763 - +0.2 2,609,832 + -0.4 2,609,892 + -0.3 2,609,905 - +0.0 2,609,905 - -0.1 2,610,017 + -0.1 2,610,017 + +0.4 2,610,126 + CA265_RS10540 0.35 -2.1 2,610,127 - CA265_RS10540 0.36 -0.6 2,610,216 + -0.0 2,610,228 + +0.1 2,610,258 + -0.2 2,610,266 + +0.1 2,610,266 + -0.1 2,610,268 + -0.4 2,610,268 + -0.7 2,610,296 - -0.4 2,610,394 + +0.4 2,610,395 - +0.9 2,610,412 - +0.5 2,610,436 - -0.6 2,610,481 + +0.1 2,610,589 - -0.5 2,610,592 + -0.0 2,610,656 + -0.5 2,610,710 + -0.9 2,610,787 + -0.3 2,610,787 + +0.3 2,610,817 + +1.7 2,610,822 + -0.6 2,610,847 + -0.2 2,610,868 + +0.6 2,610,877 - +0.4 2,610,884 + -0.2 2,610,885 - +0.3 2,610,992 + +1.1 2,610,992 + -0.3 2,610,992 + +0.3 2,610,992 + -0.8 2,610,993 - -0.2 2,611,025 + -0.3 2,611,053 - +0.5 2,611,072 - +0.2 2,611,115 - -0.3
Or see this region's nucleotide sequence