Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10490 and CA265_RS10495 are separated by 543 nucleotides CA265_RS10495 and CA265_RS10500 are separated by 185 nucleotides
CA265_RS10490: CA265_RS10490 - PhnA protein, at 2,598,086 to 2,598,664
_RS10490
CA265_RS10495: CA265_RS10495 - hypothetical protein, at 2,599,208 to 2,599,399
_RS10495
CA265_RS10500: CA265_RS10500 - arginine decarboxylase, at 2,599,585 to 2,600,973
_RS10500
Position (kb)
2599
2600 Strain fitness (log2 ratio)
-1
0
1 at 2598.277 kb on - strand, within CA265_RS10490 at 2598.370 kb on + strand, within CA265_RS10490 at 2598.450 kb on + strand, within CA265_RS10490 at 2598.527 kb on - strand, within CA265_RS10490 at 2598.547 kb on + strand, within CA265_RS10490 at 2598.561 kb on + strand, within CA265_RS10490 at 2598.561 kb on + strand, within CA265_RS10490 at 2598.562 kb on - strand, within CA265_RS10490 at 2598.610 kb on + strand at 2598.634 kb on - strand at 2598.648 kb on - strand at 2598.662 kb on + strand at 2598.665 kb on + strand at 2598.665 kb on + strand at 2598.665 kb on + strand at 2598.665 kb on + strand at 2598.666 kb on - strand at 2598.690 kb on - strand at 2598.694 kb on + strand at 2598.698 kb on + strand at 2598.699 kb on - strand at 2598.718 kb on - strand at 2598.760 kb on - strand at 2598.775 kb on - strand at 2598.775 kb on - strand at 2598.939 kb on + strand at 2599.057 kb on + strand at 2599.082 kb on - strand at 2599.191 kb on + strand at 2599.221 kb on + strand at 2599.222 kb on - strand at 2599.294 kb on + strand, within CA265_RS10495 at 2599.304 kb on + strand, within CA265_RS10495 at 2599.305 kb on - strand, within CA265_RS10495 at 2599.397 kb on + strand at 2599.414 kb on - strand at 2599.477 kb on - strand at 2599.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,598,277 - CA265_RS10490 0.33 +0.4 2,598,370 + CA265_RS10490 0.49 -0.1 2,598,450 + CA265_RS10490 0.63 -0.5 2,598,527 - CA265_RS10490 0.76 +1.1 2,598,547 + CA265_RS10490 0.80 -0.3 2,598,561 + CA265_RS10490 0.82 +0.1 2,598,561 + CA265_RS10490 0.82 +0.1 2,598,562 - CA265_RS10490 0.82 +0.0 2,598,610 + +0.4 2,598,634 - -1.1 2,598,648 - +0.5 2,598,662 + -0.5 2,598,665 + -0.2 2,598,665 + -0.3 2,598,665 + -0.7 2,598,665 + +0.0 2,598,666 - -0.6 2,598,690 - +0.1 2,598,694 + +0.1 2,598,698 + -0.9 2,598,699 - -0.3 2,598,718 - +0.4 2,598,760 - +0.3 2,598,775 - +0.2 2,598,775 - -0.9 2,598,939 + +0.2 2,599,057 + -0.2 2,599,082 - -0.3 2,599,191 + +0.7 2,599,221 + +0.7 2,599,222 - -0.0 2,599,294 + CA265_RS10495 0.45 -0.8 2,599,304 + CA265_RS10495 0.50 +0.1 2,599,305 - CA265_RS10495 0.51 -1.4 2,599,397 + -1.3 2,599,414 - -0.2 2,599,477 - -0.1 2,599,477 - -0.3
Or see this region's nucleotide sequence