Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10470 and CA265_RS10475 are separated by 124 nucleotides CA265_RS10475 and CA265_RS10480 are separated by 48 nucleotides CA265_RS10480 and CA265_RS10485 overlap by 14 nucleotides CA265_RS10485 and CA265_RS10490 are separated by 127 nucleotides
CA265_RS10470: CA265_RS10470 - amidinotransferase, at 2,594,861 to 2,596,306
_RS10470
CA265_RS10475: CA265_RS10475 - amidinotransferase, at 2,596,431 to 2,597,345
_RS10475
CA265_RS10480: CA265_RS10480 - hypothetical protein, at 2,597,394 to 2,597,708
_RS10480
CA265_RS10485: CA265_RS10485 - hypothetical protein, at 2,597,695 to 2,597,958
_RS10485
CA265_RS10490: CA265_RS10490 - PhnA protein, at 2,598,086 to 2,598,664
_RS10490
Position (kb)
2596
2597
2598 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2595.474 kb on - strand, within CA265_RS10470 at 2595.476 kb on - strand, within CA265_RS10470 at 2595.477 kb on + strand, within CA265_RS10470 at 2595.537 kb on + strand, within CA265_RS10470 at 2595.537 kb on + strand, within CA265_RS10470 at 2595.620 kb on - strand, within CA265_RS10470 at 2595.628 kb on - strand, within CA265_RS10470 at 2595.685 kb on - strand, within CA265_RS10470 at 2595.746 kb on + strand at 2595.746 kb on + strand, within CA265_RS10470 at 2595.747 kb on - strand, within CA265_RS10470 at 2595.747 kb on - strand, within CA265_RS10470 at 2595.761 kb on - strand, within CA265_RS10470 at 2595.827 kb on + strand, within CA265_RS10470 at 2595.829 kb on + strand, within CA265_RS10470 at 2595.830 kb on - strand, within CA265_RS10470 at 2595.902 kb on - strand, within CA265_RS10470 at 2595.902 kb on - strand, within CA265_RS10470 at 2595.902 kb on - strand, within CA265_RS10470 at 2595.992 kb on - strand, within CA265_RS10470 at 2595.992 kb on - strand, within CA265_RS10470 at 2596.150 kb on - strand, within CA265_RS10470 at 2596.159 kb on - strand, within CA265_RS10470 at 2596.194 kb on + strand at 2596.246 kb on - strand at 2596.290 kb on + strand at 2596.290 kb on + strand at 2596.291 kb on - strand at 2596.301 kb on - strand at 2596.323 kb on + strand at 2596.323 kb on + strand at 2596.324 kb on - strand at 2596.325 kb on + strand at 2596.355 kb on + strand at 2596.378 kb on - strand at 2596.392 kb on - strand at 2596.401 kb on + strand at 2596.402 kb on - strand at 2596.439 kb on + strand at 2596.440 kb on - strand at 2596.448 kb on + strand at 2596.624 kb on + strand, within CA265_RS10475 at 2596.625 kb on - strand, within CA265_RS10475 at 2596.647 kb on - strand, within CA265_RS10475 at 2596.792 kb on + strand, within CA265_RS10475 at 2596.843 kb on + strand, within CA265_RS10475 at 2596.847 kb on - strand, within CA265_RS10475 at 2596.869 kb on + strand, within CA265_RS10475 at 2596.879 kb on + strand, within CA265_RS10475 at 2596.980 kb on + strand, within CA265_RS10475 at 2597.070 kb on - strand, within CA265_RS10475 at 2597.070 kb on - strand, within CA265_RS10475 at 2597.081 kb on - strand, within CA265_RS10475 at 2597.081 kb on - strand, within CA265_RS10475 at 2597.081 kb on - strand, within CA265_RS10475 at 2597.150 kb on - strand, within CA265_RS10475 at 2597.158 kb on - strand, within CA265_RS10475 at 2597.179 kb on + strand, within CA265_RS10475 at 2597.260 kb on - strand at 2597.349 kb on + strand at 2597.365 kb on - strand at 2597.544 kb on + strand, within CA265_RS10480 at 2597.544 kb on + strand, within CA265_RS10480 at 2597.577 kb on - strand, within CA265_RS10480 at 2597.577 kb on - strand, within CA265_RS10480 at 2597.586 kb on - strand, within CA265_RS10480 at 2597.608 kb on + strand, within CA265_RS10480 at 2597.609 kb on - strand, within CA265_RS10480 at 2597.696 kb on + strand at 2597.696 kb on + strand at 2597.710 kb on - strand at 2597.715 kb on + strand at 2597.718 kb on + strand at 2597.801 kb on - strand, within CA265_RS10485 at 2597.812 kb on - strand, within CA265_RS10485 at 2597.832 kb on + strand, within CA265_RS10485 at 2597.833 kb on - strand, within CA265_RS10485 at 2597.892 kb on + strand, within CA265_RS10485 at 2597.893 kb on - strand, within CA265_RS10485 at 2597.973 kb on - strand at 2598.085 kb on + strand at 2598.085 kb on + strand at 2598.086 kb on - strand at 2598.135 kb on + strand at 2598.135 kb on + strand at 2598.136 kb on - strand at 2598.156 kb on + strand, within CA265_RS10490 at 2598.165 kb on - strand, within CA265_RS10490 at 2598.277 kb on - strand, within CA265_RS10490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,595,474 - CA265_RS10470 0.42 -1.4 2,595,476 - CA265_RS10470 0.43 -0.5 2,595,477 + CA265_RS10470 0.43 -1.3 2,595,537 + CA265_RS10470 0.47 -1.4 2,595,537 + CA265_RS10470 0.47 -1.0 2,595,620 - CA265_RS10470 0.52 -2.1 2,595,628 - CA265_RS10470 0.53 -2.5 2,595,685 - CA265_RS10470 0.57 -2.2 2,595,746 + +1.1 2,595,746 + CA265_RS10470 0.61 -2.5 2,595,747 - CA265_RS10470 0.61 +0.4 2,595,747 - CA265_RS10470 0.61 -0.9 2,595,761 - CA265_RS10470 0.62 -1.2 2,595,827 + CA265_RS10470 0.67 -1.1 2,595,829 + CA265_RS10470 0.67 -1.7 2,595,830 - CA265_RS10470 0.67 -0.4 2,595,902 - CA265_RS10470 0.72 -1.2 2,595,902 - CA265_RS10470 0.72 -0.7 2,595,902 - CA265_RS10470 0.72 -3.3 2,595,992 - CA265_RS10470 0.78 -1.9 2,595,992 - CA265_RS10470 0.78 -2.5 2,596,150 - CA265_RS10470 0.89 -0.4 2,596,159 - CA265_RS10470 0.90 -2.5 2,596,194 + -1.5 2,596,246 - -1.3 2,596,290 + -1.2 2,596,290 + -1.5 2,596,291 - -2.3 2,596,301 - -1.0 2,596,323 + +0.1 2,596,323 + -0.7 2,596,324 - -0.6 2,596,325 + -0.7 2,596,355 + -0.2 2,596,378 - +0.8 2,596,392 - -1.3 2,596,401 + +0.1 2,596,402 - -0.4 2,596,439 + -0.4 2,596,440 - -2.6 2,596,448 + -0.9 2,596,624 + CA265_RS10475 0.21 -1.6 2,596,625 - CA265_RS10475 0.21 -0.5 2,596,647 - CA265_RS10475 0.24 -1.1 2,596,792 + CA265_RS10475 0.39 +0.3 2,596,843 + CA265_RS10475 0.45 -1.8 2,596,847 - CA265_RS10475 0.45 -1.2 2,596,869 + CA265_RS10475 0.48 -1.0 2,596,879 + CA265_RS10475 0.49 -1.3 2,596,980 + CA265_RS10475 0.60 +0.1 2,597,070 - CA265_RS10475 0.70 -1.0 2,597,070 - CA265_RS10475 0.70 -2.1 2,597,081 - CA265_RS10475 0.71 -0.6 2,597,081 - CA265_RS10475 0.71 -1.0 2,597,081 - CA265_RS10475 0.71 -0.7 2,597,150 - CA265_RS10475 0.79 -2.1 2,597,158 - CA265_RS10475 0.79 -0.3 2,597,179 + CA265_RS10475 0.82 -0.5 2,597,260 - -0.6 2,597,349 + -2.7 2,597,365 - +0.2 2,597,544 + CA265_RS10480 0.48 +0.9 2,597,544 + CA265_RS10480 0.48 -0.3 2,597,577 - CA265_RS10480 0.58 -0.5 2,597,577 - CA265_RS10480 0.58 +1.3 2,597,586 - CA265_RS10480 0.61 +0.1 2,597,608 + CA265_RS10480 0.68 -0.6 2,597,609 - CA265_RS10480 0.68 -0.8 2,597,696 + -0.1 2,597,696 + +0.7 2,597,710 - +0.1 2,597,715 + -1.4 2,597,718 + +0.0 2,597,801 - CA265_RS10485 0.40 +0.2 2,597,812 - CA265_RS10485 0.44 -1.1 2,597,832 + CA265_RS10485 0.52 -0.3 2,597,833 - CA265_RS10485 0.52 +0.5 2,597,892 + CA265_RS10485 0.75 -0.7 2,597,893 - CA265_RS10485 0.75 -0.5 2,597,973 - -0.3 2,598,085 + +0.6 2,598,085 + +0.5 2,598,086 - +0.0 2,598,135 + -0.4 2,598,135 + +0.3 2,598,136 - -0.6 2,598,156 + CA265_RS10490 0.12 +0.0 2,598,165 - CA265_RS10490 0.14 -3.6 2,598,277 - CA265_RS10490 0.33 +0.4
Or see this region's nucleotide sequence