Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09715 and CA265_RS09720 are separated by 143 nucleotides CA265_RS09720 and CA265_RS09725 are separated by 174 nucleotides
CA265_RS09715: CA265_RS09715 - polyphosphate--nucleotide phosphotransferase, at 2,394,283 to 2,395,161
_RS09715
CA265_RS09720: CA265_RS09720 - transglycosylase, at 2,395,305 to 2,397,755
_RS09720
CA265_RS09725: CA265_RS09725 - tolB protein precursor, at 2,397,930 to 2,401,118
_RS09725
Position (kb)
2395
2396
2397
2398 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2394.482 kb on + strand, within CA265_RS09715 at 2394.494 kb on + strand, within CA265_RS09715 at 2394.673 kb on - strand, within CA265_RS09715 at 2394.718 kb on - strand, within CA265_RS09715 at 2394.801 kb on + strand, within CA265_RS09715 at 2394.801 kb on + strand, within CA265_RS09715 at 2394.805 kb on + strand, within CA265_RS09715 at 2394.873 kb on + strand, within CA265_RS09715 at 2394.874 kb on - strand, within CA265_RS09715 at 2394.890 kb on + strand, within CA265_RS09715 at 2394.891 kb on - strand, within CA265_RS09715 at 2394.891 kb on - strand, within CA265_RS09715 at 2394.925 kb on + strand, within CA265_RS09715 at 2394.926 kb on - strand, within CA265_RS09715 at 2394.926 kb on - strand, within CA265_RS09715 at 2395.006 kb on + strand, within CA265_RS09715 at 2395.057 kb on + strand, within CA265_RS09715 at 2395.060 kb on - strand, within CA265_RS09715 at 2395.138 kb on + strand at 2395.734 kb on - strand, within CA265_RS09720 at 2395.756 kb on + strand at 2395.757 kb on - strand, within CA265_RS09720 at 2395.806 kb on + strand, within CA265_RS09720 at 2395.811 kb on + strand, within CA265_RS09720 at 2395.812 kb on - strand, within CA265_RS09720 at 2395.834 kb on - strand, within CA265_RS09720 at 2395.866 kb on + strand, within CA265_RS09720 at 2395.866 kb on + strand, within CA265_RS09720 at 2395.938 kb on - strand, within CA265_RS09720 at 2395.949 kb on + strand, within CA265_RS09720 at 2395.972 kb on - strand, within CA265_RS09720 at 2395.972 kb on - strand, within CA265_RS09720 at 2395.972 kb on - strand, within CA265_RS09720 at 2395.972 kb on - strand, within CA265_RS09720 at 2395.974 kb on - strand, within CA265_RS09720 at 2396.036 kb on + strand, within CA265_RS09720 at 2396.036 kb on + strand, within CA265_RS09720 at 2396.040 kb on + strand, within CA265_RS09720 at 2396.060 kb on + strand, within CA265_RS09720 at 2396.066 kb on - strand, within CA265_RS09720 at 2396.137 kb on - strand, within CA265_RS09720 at 2396.160 kb on + strand, within CA265_RS09720 at 2396.199 kb on + strand, within CA265_RS09720 at 2396.303 kb on + strand, within CA265_RS09720 at 2396.303 kb on + strand, within CA265_RS09720 at 2396.304 kb on - strand, within CA265_RS09720 at 2396.331 kb on + strand, within CA265_RS09720 at 2396.474 kb on + strand, within CA265_RS09720 at 2396.513 kb on - strand, within CA265_RS09720 at 2396.529 kb on + strand, within CA265_RS09720 at 2396.613 kb on - strand, within CA265_RS09720 at 2396.727 kb on - strand, within CA265_RS09720 at 2396.799 kb on + strand, within CA265_RS09720 at 2396.851 kb on + strand, within CA265_RS09720 at 2396.902 kb on + strand, within CA265_RS09720 at 2396.938 kb on + strand, within CA265_RS09720 at 2397.146 kb on + strand, within CA265_RS09720 at 2397.265 kb on + strand, within CA265_RS09720 at 2397.277 kb on - strand, within CA265_RS09720 at 2397.286 kb on + strand, within CA265_RS09720 at 2397.413 kb on - strand, within CA265_RS09720 at 2397.437 kb on + strand, within CA265_RS09720 at 2397.441 kb on + strand, within CA265_RS09720 at 2397.470 kb on + strand, within CA265_RS09720 at 2397.515 kb on + strand at 2397.516 kb on - strand at 2397.535 kb on + strand at 2397.660 kb on - strand at 2397.768 kb on + strand at 2397.781 kb on - strand at 2397.789 kb on + strand at 2397.790 kb on - strand at 2397.811 kb on + strand at 2397.839 kb on + strand at 2397.865 kb on - strand at 2397.929 kb on + strand at 2397.930 kb on - strand at 2397.930 kb on - strand at 2397.944 kb on + strand at 2397.949 kb on + strand at 2397.950 kb on - strand at 2397.970 kb on + strand at 2397.971 kb on - strand at 2397.984 kb on - strand at 2398.012 kb on - strand at 2398.030 kb on + strand at 2398.031 kb on - strand at 2398.087 kb on - strand at 2398.087 kb on - strand at 2398.089 kb on + strand at 2398.089 kb on + strand at 2398.172 kb on + strand at 2398.204 kb on - strand at 2398.208 kb on + strand at 2398.209 kb on - strand at 2398.301 kb on - strand, within CA265_RS09725 at 2398.400 kb on + strand, within CA265_RS09725 at 2398.401 kb on - strand, within CA265_RS09725 at 2398.535 kb on - strand at 2398.596 kb on + strand, within CA265_RS09725 at 2398.596 kb on + strand, within CA265_RS09725 at 2398.597 kb on - strand, within CA265_RS09725 at 2398.600 kb on + strand, within CA265_RS09725 at 2398.601 kb on - strand, within CA265_RS09725 at 2398.626 kb on - strand, within CA265_RS09725 at 2398.656 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725 at 2398.745 kb on + strand, within CA265_RS09725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,394,482 + CA265_RS09715 0.23 -0.3 2,394,494 + CA265_RS09715 0.24 +0.3 2,394,673 - CA265_RS09715 0.44 +0.3 2,394,718 - CA265_RS09715 0.49 +0.0 2,394,801 + CA265_RS09715 0.59 +0.2 2,394,801 + CA265_RS09715 0.59 +0.7 2,394,805 + CA265_RS09715 0.59 -0.6 2,394,873 + CA265_RS09715 0.67 -0.1 2,394,874 - CA265_RS09715 0.67 -1.8 2,394,890 + CA265_RS09715 0.69 -0.5 2,394,891 - CA265_RS09715 0.69 -0.5 2,394,891 - CA265_RS09715 0.69 -1.3 2,394,925 + CA265_RS09715 0.73 +0.6 2,394,926 - CA265_RS09715 0.73 -0.4 2,394,926 - CA265_RS09715 0.73 -0.5 2,395,006 + CA265_RS09715 0.82 -0.6 2,395,057 + CA265_RS09715 0.88 -0.2 2,395,060 - CA265_RS09715 0.88 +0.4 2,395,138 + -0.0 2,395,734 - CA265_RS09720 0.18 -1.3 2,395,756 + -0.6 2,395,757 - CA265_RS09720 0.18 -1.3 2,395,806 + CA265_RS09720 0.20 -1.3 2,395,811 + CA265_RS09720 0.21 -2.0 2,395,812 - CA265_RS09720 0.21 -2.8 2,395,834 - CA265_RS09720 0.22 +0.0 2,395,866 + CA265_RS09720 0.23 -2.7 2,395,866 + CA265_RS09720 0.23 -2.2 2,395,938 - CA265_RS09720 0.26 -2.3 2,395,949 + CA265_RS09720 0.26 -1.6 2,395,972 - CA265_RS09720 0.27 -2.9 2,395,972 - CA265_RS09720 0.27 -2.5 2,395,972 - CA265_RS09720 0.27 -1.6 2,395,972 - CA265_RS09720 0.27 -2.8 2,395,974 - CA265_RS09720 0.27 -4.2 2,396,036 + CA265_RS09720 0.30 -2.9 2,396,036 + CA265_RS09720 0.30 -3.3 2,396,040 + CA265_RS09720 0.30 -2.7 2,396,060 + CA265_RS09720 0.31 -2.4 2,396,066 - CA265_RS09720 0.31 -3.3 2,396,137 - CA265_RS09720 0.34 -3.7 2,396,160 + CA265_RS09720 0.35 -1.8 2,396,199 + CA265_RS09720 0.36 -0.7 2,396,303 + CA265_RS09720 0.41 -2.0 2,396,303 + CA265_RS09720 0.41 -0.6 2,396,304 - CA265_RS09720 0.41 -2.9 2,396,331 + CA265_RS09720 0.42 -1.8 2,396,474 + CA265_RS09720 0.48 -3.6 2,396,513 - CA265_RS09720 0.49 -3.3 2,396,529 + CA265_RS09720 0.50 -1.7 2,396,613 - CA265_RS09720 0.53 -2.6 2,396,727 - CA265_RS09720 0.58 -1.0 2,396,799 + CA265_RS09720 0.61 -2.7 2,396,851 + CA265_RS09720 0.63 -2.6 2,396,902 + CA265_RS09720 0.65 -1.8 2,396,938 + CA265_RS09720 0.67 -3.8 2,397,146 + CA265_RS09720 0.75 -2.9 2,397,265 + CA265_RS09720 0.80 -3.1 2,397,277 - CA265_RS09720 0.80 -2.9 2,397,286 + CA265_RS09720 0.81 -3.4 2,397,413 - CA265_RS09720 0.86 -0.6 2,397,437 + CA265_RS09720 0.87 -1.3 2,397,441 + CA265_RS09720 0.87 -3.4 2,397,470 + CA265_RS09720 0.88 -0.8 2,397,515 + -0.7 2,397,516 - -1.9 2,397,535 + -0.8 2,397,660 - +0.6 2,397,768 + +0.3 2,397,781 - -0.9 2,397,789 + -0.5 2,397,790 - -0.1 2,397,811 + +0.3 2,397,839 + -0.1 2,397,865 - -0.2 2,397,929 + +0.3 2,397,930 - -0.2 2,397,930 - +0.4 2,397,944 + +0.1 2,397,949 + -0.5 2,397,950 - -0.4 2,397,970 + +1.2 2,397,971 - +0.7 2,397,984 - -0.7 2,398,012 - -0.6 2,398,030 + +0.0 2,398,031 - +0.1 2,398,087 - +0.1 2,398,087 - +0.0 2,398,089 + +0.8 2,398,089 + +0.2 2,398,172 + -0.1 2,398,204 - -0.8 2,398,208 + -0.1 2,398,209 - -0.7 2,398,301 - CA265_RS09725 0.12 -0.2 2,398,400 + CA265_RS09725 0.15 +0.1 2,398,401 - CA265_RS09725 0.15 +0.0 2,398,535 - +2.0 2,398,596 + CA265_RS09725 0.21 +1.6 2,398,596 + CA265_RS09725 0.21 +0.1 2,398,597 - CA265_RS09725 0.21 +0.0 2,398,600 + CA265_RS09725 0.21 +0.5 2,398,601 - CA265_RS09725 0.21 -0.1 2,398,626 - CA265_RS09725 0.22 +0.6 2,398,656 + CA265_RS09725 0.23 +0.4 2,398,745 + CA265_RS09725 0.26 +0.9 2,398,745 + CA265_RS09725 0.26 -0.6 2,398,745 + CA265_RS09725 0.26 +1.2 2,398,745 + CA265_RS09725 0.26 +0.1
Or see this region's nucleotide sequence