Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09520 and CA265_RS09525 are separated by 159 nucleotides CA265_RS09525 and CA265_RS09530 are separated by 80 nucleotides
CA265_RS09520: CA265_RS09520 - site-specific tyrosine recombinase XerD, at 2,357,706 to 2,358,605
_RS09520
CA265_RS09525: CA265_RS09525 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 2,358,765 to 2,359,652
_RS09525
CA265_RS09530: CA265_RS09530 - riboflavin biosynthesis protein RibD, at 2,359,733 to 2,360,785
_RS09530
Position (kb)
2358
2359
2360 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2357.804 kb on + strand, within CA265_RS09520 at 2357.856 kb on - strand, within CA265_RS09520 at 2357.857 kb on + strand, within CA265_RS09520 at 2357.873 kb on - strand, within CA265_RS09520 at 2357.882 kb on + strand, within CA265_RS09520 at 2357.944 kb on + strand, within CA265_RS09520 at 2357.961 kb on + strand, within CA265_RS09520 at 2357.965 kb on + strand, within CA265_RS09520 at 2357.966 kb on - strand, within CA265_RS09520 at 2358.082 kb on + strand, within CA265_RS09520 at 2358.083 kb on - strand, within CA265_RS09520 at 2358.083 kb on - strand, within CA265_RS09520 at 2358.130 kb on + strand, within CA265_RS09520 at 2358.135 kb on + strand, within CA265_RS09520 at 2358.301 kb on + strand, within CA265_RS09520 at 2358.302 kb on - strand, within CA265_RS09520 at 2358.308 kb on + strand, within CA265_RS09520 at 2358.308 kb on + strand, within CA265_RS09520 at 2358.371 kb on + strand, within CA265_RS09520 at 2358.474 kb on - strand, within CA265_RS09520 at 2358.500 kb on - strand, within CA265_RS09520 at 2358.528 kb on - strand at 2358.528 kb on - strand at 2358.528 kb on - strand at 2358.530 kb on + strand at 2358.545 kb on + strand at 2358.564 kb on + strand at 2358.604 kb on - strand at 2358.610 kb on - strand at 2358.652 kb on - strand at 2358.652 kb on - strand at 2358.695 kb on - strand at 2358.709 kb on + strand at 2358.852 kb on + strand at 2358.916 kb on + strand, within CA265_RS09525 at 2358.942 kb on - strand, within CA265_RS09525 at 2358.963 kb on - strand, within CA265_RS09525 at 2358.963 kb on - strand, within CA265_RS09525 at 2358.969 kb on - strand, within CA265_RS09525 at 2358.969 kb on - strand, within CA265_RS09525 at 2358.976 kb on - strand, within CA265_RS09525 at 2359.043 kb on + strand, within CA265_RS09525 at 2359.079 kb on + strand, within CA265_RS09525 at 2359.091 kb on + strand, within CA265_RS09525 at 2359.179 kb on + strand, within CA265_RS09525 at 2359.215 kb on + strand, within CA265_RS09525 at 2359.230 kb on + strand, within CA265_RS09525 at 2359.241 kb on + strand, within CA265_RS09525 at 2359.376 kb on + strand, within CA265_RS09525 at 2359.442 kb on + strand, within CA265_RS09525 at 2359.548 kb on + strand, within CA265_RS09525 at 2359.600 kb on + strand at 2359.601 kb on - strand at 2359.602 kb on + strand at 2359.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,357,804 + CA265_RS09520 0.11 +0.0 2,357,856 - CA265_RS09520 0.17 -0.8 2,357,857 + CA265_RS09520 0.17 -1.3 2,357,873 - CA265_RS09520 0.19 +0.3 2,357,882 + CA265_RS09520 0.20 -0.6 2,357,944 + CA265_RS09520 0.26 -1.3 2,357,961 + CA265_RS09520 0.28 -0.9 2,357,965 + CA265_RS09520 0.29 -0.5 2,357,966 - CA265_RS09520 0.29 +0.2 2,358,082 + CA265_RS09520 0.42 -0.7 2,358,083 - CA265_RS09520 0.42 -0.6 2,358,083 - CA265_RS09520 0.42 -0.2 2,358,130 + CA265_RS09520 0.47 -0.2 2,358,135 + CA265_RS09520 0.48 +0.1 2,358,301 + CA265_RS09520 0.66 +0.2 2,358,302 - CA265_RS09520 0.66 +1.0 2,358,308 + CA265_RS09520 0.67 -0.2 2,358,308 + CA265_RS09520 0.67 -0.5 2,358,371 + CA265_RS09520 0.74 -0.1 2,358,474 - CA265_RS09520 0.85 +0.2 2,358,500 - CA265_RS09520 0.88 -1.2 2,358,528 - -1.1 2,358,528 - -0.7 2,358,528 - -0.4 2,358,530 + -0.7 2,358,545 + -0.6 2,358,564 + -0.4 2,358,604 - +1.8 2,358,610 - +0.4 2,358,652 - +0.7 2,358,652 - +0.2 2,358,695 - +0.4 2,358,709 + -0.9 2,358,852 + -2.1 2,358,916 + CA265_RS09525 0.17 -0.4 2,358,942 - CA265_RS09525 0.20 -1.9 2,358,963 - CA265_RS09525 0.22 -1.3 2,358,963 - CA265_RS09525 0.22 -0.7 2,358,969 - CA265_RS09525 0.23 -1.0 2,358,969 - CA265_RS09525 0.23 -0.5 2,358,976 - CA265_RS09525 0.24 -1.2 2,359,043 + CA265_RS09525 0.31 -1.2 2,359,079 + CA265_RS09525 0.35 -0.4 2,359,091 + CA265_RS09525 0.37 -0.6 2,359,179 + CA265_RS09525 0.47 -0.4 2,359,215 + CA265_RS09525 0.51 -1.7 2,359,230 + CA265_RS09525 0.52 -0.7 2,359,241 + CA265_RS09525 0.54 -0.8 2,359,376 + CA265_RS09525 0.69 -0.6 2,359,442 + CA265_RS09525 0.76 +0.0 2,359,548 + CA265_RS09525 0.88 -0.3 2,359,600 + -1.1 2,359,601 - +0.5 2,359,602 + -1.2 2,359,618 - -0.9
Or see this region's nucleotide sequence